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Ahcy elisa kit :: Rat Adenosylhomocysteinase ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2885786 Ahcy elisa kit
Unit / Price
48-Strip-Wells  /  $420 +1 FREE 8GB USB
96-Strip-Wells  /  $560 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,445 +3 FREE 8GB USB
10x96-Strip-Wells  /  $4,520 +5 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Adenosylhomocysteinase (Ahcy), ELISA Kit

★Popular Item★
 Also Known As   

Rat Adenosylhomocysteinase ELISA Kit

 Product Synonym Names    Adenosylhomocysteinase; AdoHcyase; S-adenosyl-L-homocysteine hydrolase; Ahcy; 3.3.1.1
 Product Gene Name   

Ahcy elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS2885786 Testing Data    Testing Data PDF
 3D Structure    ModBase 3D Structure for P10760
 Species Reactivity    Rat
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 Assay Type    Sandwich
 Detection Range    0.156-10 ng/mL
 Sensitivity    0.089 ng/mL
 Intra-Assay CV    <=4.9%
 Inter-Assay CV    <=7.4%
 Recovery    88%
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 Preparation and Storage    For long term storage, please store the entire kit at -20 degree C.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of Ahcy elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for Ahcy purchase    MBS2885786 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Adenosylhomocysteinase (Ahcy) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.156-10 ng/mL in biological research samples containing Ahcy, with an estimated sensitivity of 0.089 ng/mL. The ELISA analytical biochemical technique of the MBS2885786 kit is based on Ahcy antibody-Ahcy antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Ahcy antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Ahcy. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for Ahcy elisa kit

   Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Sample Manual Insert of MBS2885786. Click to request current manual
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NCBI/Uniprot data below describe general gene information for Ahcy. It may not necessarily be applicable to this product.
 NCBI GI #    8392878
 NCBI GeneID    29443
 NCBI Accession #    NP_058897.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_017201.1 [Other Products]
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 UniProt Primary Accession #    P10760 [Other Products]
 UniProt Related Accession #    P10760 [Other Products]
 Molecular Weight    47,538 Da
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 NCBI Official Full Name    adenosylhomocysteinase
 NCBI Official Synonym Full Names    adenosylhomocysteinase
 NCBI Official Symbol    Ahcy [Similar Products]
 NCBI Protein Information    adenosylhomocysteinase
 UniProt Protein Name    Adenosylhomocysteinase
 UniProt Synonym Protein Names   
S-adenosyl-L-homocysteine hydrolase
 Protein Family    Adenosylhomocysteinase
 UniProt Gene Name    Ahcy [Similar Products]
 UniProt Synonym Gene Names    AdoHcyase [Similar Products]
 UniProt Entry Name    SAHH_RAT
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 NCBI Summary for Ahcy    enzyme that catalyzes the hydrolysis of S-adenosylhomocysteine to form adenosine and homocysteine [RGD, Feb 2006]
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 UniProt Comments for Ahcy    SAHH: Adenosylhomocysteine is a competitive inhibitor of S- adenosyl-L-methionine-dependent methyl transferase reactions; therefore adenosylhomocysteinase may play a key role in the control of methylations via regulation of the intracellular concentration of adenosylhomocysteine. Defects in AHCY are the cause of hypermethioninemia with S-adenosylhomocysteine hydrolase deficiency (HMAHCHD). A metabolic disorder characterized by hypermethioninemia associated with failure to thrive, mental and motor retardation, facial dysmorphism with abnormal hair and teeth, and myocardiopathy. Belongs to the adenosylhomocysteinase family.

Protein type: Hydrolase; Amino Acid Metabolism - cysteine and methionine; EC 3.3.1.1; Other Amino Acids Metabolism - selenoamino acid

Cellular Component: cytoplasm; cytosol; melanosome; neuron projection; nucleus

Molecular Function: adenosylhomocysteinase activity; adenyl nucleotide binding; copper ion binding; identical protein binding; NAD binding; protein self-association

Biological Process: chronic inflammatory response to antigenic stimulus; circadian sleep/wake cycle; one-carbon compound metabolic process; response to hypoxia; response to nutrient; S-adenosylhomocysteine catabolic process; S-adenosylmethionine cycle
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 Research Articles on Ahcy    1. report that Myc promotes upregulation of S-adenosyl homocysteine hydrolase.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with Ahcy elisa kitPathways associated with Ahcy elisa kit
 Reference Product  PubMed Publications
 Mat1a elisa kit  >6 publications with Ahcy and Mat1a
 Bhmt elisa kit  >6 publications with Ahcy and Bhmt
 Cbs elisa kit  >5 publications with Ahcy and Cbs
 Mtrr elisa kit  >4 publications with Ahcy and Mtrr
 Mat2a elisa kit  >4 publications with Ahcy and Mat2a
 Mtr elisa kit  >4 publications with Ahcy and Mtr
 Dnmt3b elisa kit  >1 publications with Ahcy and Dnmt3b
 Dnmt1 elisa kit  >1 publications with Ahcy and Dnmt1
 Products by Pathway  Pathway Diagram
 Biological Oxidations Pathway antibodies  Biological Oxidations Pathway Diagram
 Cysteine And Methionine Metabolism Pathway antibodies  Cysteine And Methionine Metabolism Pathway Diagram
 Cysteine And Methionine Metabolism Pathway antibodies  Cysteine And Methionine Metabolism Pathway Diagram
 L-methionine Degradation I (to L-homocysteine) Pathway antibodies  L-methionine Degradation I (to L-homocysteine) Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Amino Acids And Derivatives Pathway antibodies  Metabolism Of Amino Acids And Derivatives Pathway Diagram
 Methionine Degradation Pathway antibodies  Methionine Degradation Pathway Diagram
 Methionine Degradation Pathway antibodies  Methionine Degradation Pathway Diagram
 Methylation Pathway antibodies  Methylation Pathway Diagram
Diseases associated with Ahcy elisa kitOrgans/Tissues associated with Ahcy elisa kit
 Disease Name  Pubmed Publications
 Liver Diseases Antibodies  >5 publications with Ahcy and Liver Diseases
 Necrosis Antibodies  >4 publications with Ahcy and Necrosis
 Disease Models, Animal Antibodies  >4 publications with Ahcy and Disease Models, Animal
 Cardiovascular Diseases Antibodies  >3 publications with Ahcy and Cardiovascular Diseases
 Fatty Liver Antibodies  >3 publications with Ahcy and Fatty Liver
 Nervous System Diseases Antibodies  >3 publications with Ahcy and Nervous System Diseases
 Hepatitis Antibodies  >3 publications with Ahcy and Hepatitis
 Inflammation Antibodies  >2 publications with Ahcy and Inflammation
 Amino Acid Metabolism, Inborn Errors Antibodies  >2 publications with Ahcy and Amino Acid Metabolism, Inborn Errors
 Carcinoma, Hepatocellular Antibodies  >1 publications with Ahcy and Carcinoma, Hepatocellular
 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >14 publications with Ahcy and Liver
 Colon Antibodies  >4 publications with Ahcy and Colon
 Brain Antibodies  >4 publications with Ahcy and Brain
 Embryonic Tissue Antibodies  >3 publications with Ahcy and Embryonic Tissue
 Ovary Antibodies  >2 publications with Ahcy and Ovary
 Heart Antibodies  >2 publications with Ahcy and Heart
 Kidney Antibodies  >2 publications with Ahcy and Kidney
 Thymus Antibodies  >1 publications with Ahcy and Thymus
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