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AgRP elisa kit :: Rat Agouti Related Protein ELISA Kit

Scan QR to view Datasheet Catalog #    MBS762381
Unit / Price
48-Strip-Wells  /  $280 +1 FREE 8GB USB
96-Strip-Wells  /  $375 +1 FREE 8GB USB
5x96-Strip-Wells  /  $1,615 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,015 +3 FREE 8GB USB
15x96-Strip-Wells  /  $4,285 +5 FREE 8GB USB
25x96-Strip-Wells  /  $6,715 +8 FREE 8GB USB
 
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 Product Name   

Agouti Related Protein (AgRP), ELISA Kit

★Popular Item★
 Also Known As   

Rat Agouti Related Protein ELISA Kit

 Product Synonym Names    Agrp/AGRP (Agouti-related protein)/AGRT/ASIP2/ART/agouti-related protein/Agrt
 Product Gene Name   

AgRP elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS762381 COA    COA PDF
 OMIM    601665
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 Species Reactivity    Rat
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 Samples    Serum, plasma and other biological fluids.
 Assay Type    Sandwich ELISA, Double Antibody
 Detection Range    15.625-1000pg/ml
 Sensitivity    9.375pg/ml
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 Preparation and Storage    Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of AgRP elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for AgRP purchase    MBS762381 is a ready-to-use microwell, strip plate Sandwich, Double Antibody ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Agouti Related Protein (AgRP) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 15.625-1000pg/ml in biological research samples containing AgRP, with an estimated sensitivity of 9.375pg/ml. The ELISA analytical biochemical technique of the MBS762381 kit is based on AgRP antibody-AgRP antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect AgRP antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, AgRP. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for AgRP elisa kit

   Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Rat AgRP antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Rat AgRP antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Rat AgRP amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration ofRat AgRP can be calculated.
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 Typical Testing Data of AgRP elisa kit    AgRP elisa kit Typical Testing Data image
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 Typical Standard Curve of AgRP elisa kit    AgRP elisa kit Typical Standard Curve image
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Sample Manual Insert of MBS762381. Click to request current manual
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NCBI/Uniprot data below describe general gene information for AgRP. It may not necessarily be applicable to this product.
 NCBI GI #    4501995
 NCBI GeneID    181
 NCBI Accession #    NP_001129.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001138.1 [Other Products]
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 UniProt Secondary Accession #    O15459; Q2TBD9 [Other Products]
 UniProt Related Accession #    O00253 [Other Products]
 Molecular Weight    14,440 Da
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 NCBI Official Full Name    agouti-related protein
 NCBI Official Synonym Full Names    agouti related neuropeptide
 NCBI Official Symbol    AGRP [Similar Products]
 NCBI Official Synonym Symbols   
ART; AGRT; ASIP2
[Similar Products]
 NCBI Protein Information    agouti-related protein
 UniProt Protein Name    Agouti-related protein
 Protein Family    Agouti-related protein
 UniProt Gene Name    AGRP [Similar Products]
 UniProt Synonym Gene Names    AGRT; ART [Similar Products]
 UniProt Entry Name    AGRP_HUMAN
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 NCBI Summary for AgRP    This gene encodes an antagonist of the melanocortin-3 and melanocortin-4 receptor. It appears to regulate hypothalamic control of feeding behavior via melanocortin receptor and/or intracellular calcium regulation, and thus plays a role in weight homeostasis. Mutations in this gene have been associated with late on-set obesity. [provided by RefSeq, Dec 2009]
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 UniProt Comments for AgRP    AGRP: Plays a role in weight homeostasis. Involved in the control of feeding behavior through the central melanocortin system. Acts as alpha melanocyte-stimulating hormone antagonist by inhibiting cAMP production mediated by stimulation of melanocortin receptors within the hypothalamus and adrenal gland. Has very low activity with MC5R. Is an inverse agonist for MC3R and MC4R being able to suppress their constitutive activity. It promotes MC3R and MC4R endocytosis in an arrestin-dependent manner. Genetic variations in AGRP may be a cause of obesity (OBESITY). It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat.

Protein type: Hormone; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 16q22

Cellular Component: extracellular space; Golgi lumen

Molecular Function: neuropeptide hormone activity; receptor binding

Biological Process: feeding behavior; neuropeptide signaling pathway

Disease: Obesity
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 Research Articles on AgRP    1. CSF AgRP was not different in lean vs. overweight/obese subjects; however, plasma AgRP was higher in lean subjects.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with AgRP elisa kitPathways associated with AgRP elisa kit
 Reference Product  PubMed Publications
 POMC elisa kit  >623 publications with AgRP and POMC
 NPY elisa kit  >528 publications with AgRP and NPY
 MC4R elisa kit  >144 publications with AgRP and MC4R
 MC3R elisa kit  >66 publications with AgRP and MC3R
 FOS elisa kit  >43 publications with AgRP and FOS
 TRH elisa kit  >32 publications with AgRP and TRH
 LEP elisa kit  >15 publications with AgRP and LEP
 GHRL elisa kit  >3 publications with AgRP and GHRL
 SDC3 elisa kit  >3 publications with AgRP and SDC3
 Products by Pathway  Pathway Diagram
 Adipocytokine Signaling Pathway antibodies  Adipocytokine Signaling Pathway Diagram
 Adipocytokine Signaling Pathway antibodies  Adipocytokine Signaling Pathway Diagram
 Syndecan-3-mediated Signaling Events Pathway antibodies  Syndecan-3-mediated Signaling Events Pathway Diagram
Diseases associated with AgRP elisa kitOrgans/Tissues associated with AgRP elisa kit
 Disease Name  Pubmed Publications
 Obesity Antibodies  >430 publications with AgRP and Obesity
 Weight Gain Antibodies  >133 publications with AgRP and Weight Gain
 Weight Loss Antibodies  >101 publications with AgRP and Weight Loss
 Insulin Resistance Antibodies  >66 publications with AgRP and Insulin Resistance
 Disease Models, Animal Antibodies  >62 publications with AgRP and Disease Models, Animal
 Diabetes Mellitus Antibodies  >49 publications with AgRP and Diabetes Mellitus
 Hyperglycemia Antibodies  >30 publications with AgRP and Hyperglycemia
 Inflammation Antibodies  >29 publications with AgRP and Inflammation
 Hyperinsulinism Antibodies  >29 publications with AgRP and Hyperinsulinism
 Nervous System Diseases Antibodies  >25 publications with AgRP and Nervous System Diseases
 Organ/Tissue Name  Pubmed Publications
 Connective Tissue Antibodies  >75 publications with AgRP and Connective Tissue
 Kidney Antibodies  >21 publications with AgRP and Kidney
 Lung Antibodies  >3 publications with AgRP and Lung
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