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AhR elisa kit :: Rat AhR ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2500236
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $410 +1 FREE 8GB USB
96-Strip-Wells  /  $490 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,040 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,590 +4 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

AhR, ELISA Kit

★Popular Item★
 Also Known As   

Rat AhR (Aryl Hydrocarbon Receptor) ELISA Kit

 Product Gene Name   

AhR elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    600253
 3D Structure    ModBase 3D Structure for P35869
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 Species Reactivity    Rat
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Rat AhR. No significant cross-reactivity or interference between Rat AhR and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Rat AhR and all the analogues, therefore, cross reaction may still exist.
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 Samples    Serum, Plasma, Biological Fluids
 Assay Type    Sandwich
 Detection Range    0.313--20ng/mL
 Sensitivity    0.188ng/mL
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 Preparation and Storage    Store at 4 degree C.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of AhR elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for AhR purchase    MBS2500236 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the AhR, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.313--20ng/mL in biological research samples containing AhR, with an estimated sensitivity of 0.188ng/mL. The ELISA analytical biochemical technique of the MBS2500236 kit is based on AhR antibody-AhR antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect AhR antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, AhR. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma, Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for AhR elisa kit

   Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of AhR in Rat serum, plasma and other biological fluids
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat AhR. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Rat AhR and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat AhR, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Rat AhR. You can calculate the concentration of Rat AhR in the samples by comparing the OD of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of AhR elisa kit    AhR elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2500236. Click to request current manual
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NCBI/Uniprot data below describe general gene information for AhR. It may not necessarily be applicable to this product.
 NCBI GI #    4502003
 NCBI GeneID    196
 NCBI Accession #    NP_001612.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001621.4 [Other Products]
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 UniProt Primary Accession #    P35869 [Other Products]
 UniProt Secondary Accession #    Q13728; Q13803; Q13804; A4D130 [Other Products]
 UniProt Related Accession #    P35869 [Other Products]
 Molecular Weight    96,147 Da
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 NCBI Official Full Name    aryl hydrocarbon receptor
 NCBI Official Synonym Full Names    aryl hydrocarbon receptor
 NCBI Official Symbol    AHR [Similar Products]
 NCBI Official Synonym Symbols   
bHLHe76
[Similar Products]
 NCBI Protein Information    aryl hydrocarbon receptor; AH-receptor; ah receptor; aromatic hydrocarbon receptor; class E basic helix-loop-helix protein 76
 UniProt Protein Name    Aryl hydrocarbon receptor
 UniProt Synonym Protein Names   
Class E basic helix-loop-helix protein 76; bHLHe76
 Protein Family    Aldehyde reductase
 UniProt Gene Name    AHR [Similar Products]
 UniProt Synonym Gene Names    BHLHE76; Ah receptor; AhR; bHLHe76 [Similar Products]
 UniProt Entry Name    AHR_HUMAN
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 NCBI Summary for AhR    This gene encodes a ligand-activated transcription factor involved in the regulation of biological responses to planar aromatic hydrocarbons. This receptor has been shown to regulate xenobiotic-metabolizing enzymes such as cytochrome P450. Its ligands included a variety of aromatic hydrocarbons. [provided by RefSeq, Jul 2008]
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 UniProt Comments for AhR    AHR: a nuclear receptor for aryl hydrocarbons involved in xenobiotic metabolism, cell cycle regulation, and development in response to both endogenous and environmental signals. AhR was initially identified as a receptor for dioxins, which are environmental pollutants generated by waste incineration and other industrial processes . AhR ligands include polycyclic aromatic hydrocarbons, including the carcinogen benzo(a)pyrene and other components of cigarette smoke. Naturally occurring AhR ligands include flavonoids, which are aromatic plant secondary compounds commonly found in vegetables and fruits. Cytoplasmic aryl hydrocarbon receptors are found in protein complexes with heat shock proteins. Upon ligand binding, AhR dissociates from heat shock proteins and translocate to the nucleus where it dimerizes with AhR nuclear translocator (ARNT, HIF-1b). The AhR/ARNT heterodimer binds to nuclear xenobiotic response elements to control the expression of genes associated with xenobiotic metabolism, including several cytochrome P450 genes. AhR is ubiquitously expressed and is thought to play a role in regulation of cell proliferation and differentiation, cytokine expression, and xenobiotic metabolism. Research studies link AhR activity with the control of regulatory T-cell and T-helper 17 cell differentiation, regulation of the inflammatory response, and the onset of lung cancer.

Protein type: DNA-binding; Transcription factor; Nuclear receptor

Chromosomal Location of Human Ortholog: 7p15

Cellular Component: nucleoplasm; transcription factor complex; cytoplasm; nucleus

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein dimerization activity; ligand-dependent nuclear receptor activity; protein binding; signal transducer activity; DNA binding; protein heterodimerization activity; sequence-specific DNA binding; Hsp90 protein binding; transcription factor binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; prostate gland development; blood vessel development; intracellular receptor-mediated signaling pathway; apoptosis; response to toxin; positive regulation of transcription, DNA-dependent; negative regulation of transcription from RNA polymerase II promoter; cell cycle; regulation of transcription from RNA polymerase II promoter; response to xenobiotic stimulus; regulation of transcription, DNA-dependent; regulation of gene expression; xenobiotic metabolic process; regulation of B cell proliferation; positive regulation of transcription from RNA polymerase II promoter; circadian regulation of gene expression; positive regulation of transcriptional preinitiation complex assembly; negative regulation of transcription, DNA-dependent
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 Research Articles on AhR    1. The current review contributes to a better understanding of the role of AhR and its signaling pathway in CD4 helper T cell mediated inflammatory disorder. [review]
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with AhR elisa kitPathways associated with AhR elisa kit
 Reference Product  PubMed Publications
 CYP1A1 elisa kit  >1387 publications with AhR and CYP1A1
 ARNT elisa kit  >641 publications with AhR and ARNT
 CYP1B1 elisa kit  >382 publications with AhR and CYP1B1
 SRC elisa kit  >46 publications with AhR and SRC
 AIP elisa kit  >28 publications with AhR and AIP
 RELA elisa kit  >18 publications with AhR and RELA
 ESR1 elisa kit  >13 publications with AhR and ESR1
 RB1 elisa kit  >6 publications with AhR and RB1
 HSP90AA1 elisa kit  >3 publications with AhR and HSP90AA1
 SMARCA4 elisa kit  >2 publications with AhR and SMARCA4
 Products by Pathway  Pathway Diagram
 Adipogenesis Pathway antibodies  Adipogenesis Pathway Diagram
 AhR Pathway antibodies  AhR Pathway Diagram
 Integrated Breast Cancer Pathway antibodies  Integrated Breast Cancer Pathway Diagram
Diseases associated with AhR elisa kitOrgans/Tissues associated with AhR elisa kit
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >1345 publications with AhR and Inflammation
 Liver Diseases Antibodies  >348 publications with AhR and Liver Diseases
 Breast Neoplasms Antibodies  >261 publications with AhR and Breast Neoplasms
 Liver Neoplasms Antibodies  >256 publications with AhR and Liver Neoplasms
 Necrosis Antibodies  >212 publications with AhR and Necrosis
 Pneumonia Antibodies  >154 publications with AhR and Pneumonia
 Hyperplasia Antibodies  >150 publications with AhR and Hyperplasia
 Neoplasms, Experimental Antibodies  >143 publications with AhR and Neoplasms, Experimental
 Kidney Diseases Antibodies  >122 publications with AhR and Kidney Diseases
 Hypertension Antibodies  >113 publications with AhR and Hypertension
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >1630 publications with AhR and Blood
 Lung Antibodies  >1438 publications with AhR and Lung
 Liver Antibodies  >1085 publications with AhR and Liver
 Muscle Antibodies  >403 publications with AhR and Muscle
 Heart Antibodies  >386 publications with AhR and Heart
 Kidney Antibodies  >379 publications with AhR and Kidney
 Vascular Antibodies  >287 publications with AhR and Vascular
 Skin Antibodies  >276 publications with AhR and Skin
 Brain Antibodies  >257 publications with AhR and Brain
 Bone Antibodies  >222 publications with AhR and Bone
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