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a1AT elisa kit :: Rat Alpha-1-Antitrypsin (a1AT) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2020625 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells  /  $270 +1 FREE 8GB USB
48-Strip-Wells  /  $505 +1 FREE 8GB USB
96-Strip-Wells  /  $685 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,775 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,035 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Alpha-1-Antitrypsin (a1AT), ELISA Kit

★Popular Item★
 Also Known As   

Alpha-1-Antitrypsin (a1AT) ELISA Kit

 Product Synonym Names    SERPINA1; SPAAT; A1-AT; PI; Serpin Peptidase Inhibitor,Clade A(Alpha-1 Antiproteinase/AntiTrypsin)Member 1; Alpha-1 protease inhibitor; Short peptide from AAT; Serpin A1
 Product Gene Name   

a1AT elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    107400
 3D Structure    ModBase 3D Structure for P01009
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 Species Reactivity    Rat
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Alpha-1-Antitrypsin (a1AT).
No significant cross-reactivity or interference between Alpha-1-Antitrypsin (a1AT) and analogues was observed.
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 Assay Type    Double-antibody Sandwich
 Samples    Serum, Plasma and Other Biological Fluids
 Detection Range    7.81-500ng/mL
 Sensitivity    < 3.5ng/mL
 Application    Enzyme-linked immunosorbent assay for Antigen Detection.
 Intra-assay Precision (Precision within an assay)    3 samples with low, middle and high level Alpha-1-Antitrypsin (a1AT) were tested 20 times on one plate, respectively.
 Inter-assay Precision (Precision between assays)    3 samples with low, middle and high level Alpha-1-Antitrypsin (a1AT) were tested on 3 different plates, 8 replicates in each plate.
 CV(%) =    SD/meanX100
 Intra-Assay    CV<10%
 Inter-Assay    CV<12%
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 Preparation and Storage    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
 ISO Certification    Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
 Supply Chain Verification    Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available. Please inquire.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of a1AT elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for a1AT purchase    MBS2020625 is a ready-to-use microwell, strip plate Double-antibody Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Alpha-1-Antitrypsin (a1AT) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 7.81-500ng/mL in biological research samples containing a1AT, with an estimated sensitivity of < 3.5ng/mL. The ELISA analytical biochemical technique of the MBS2020625 kit is based on a1AT antibody-a1AT antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect a1AT antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, a1AT. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma and Other Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for a1AT elisa kit

   The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alpha-1-Antitrypsin (a1AT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Alpha-1-Antitrypsin (a1AT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Alpha-1-Antitrypsin (a1AT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Alpha-1-Antitrypsin (a1AT) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
 Product Categories/Family for a1AT elisa kit    Enzyme & Kinase; Infection immunity; Hepatology
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 Typical Testing Data/Standard Curve (for reference only) of a1AT elisa kit    a1AT elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2020625. Click to request current manual
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NCBI/Uniprot data below describe general gene information for a1AT. It may not necessarily be applicable to this product.
 NCBI GI #    50363221
 NCBI GeneID    5265
 NCBI Accession #    NP_001002235.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001002235.2 [Other Products]
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 UniProt Primary Accession #    P01009 [Other Products]
 UniProt Secondary Accession #    Q0PVP5; Q13672; Q53XB8; Q5U0M1; Q7M4R2; Q86U18; Q86U19; Q96BF9; Q96ES1; A6PX14; B2RDQ8 [Other Products]
 UniProt Related Accession #    P01009 [Other Products]
 Molecular Weight    34,755 Da
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 NCBI Official Full Name    alpha-1-antitrypsin
 NCBI Official Synonym Full Names    serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1
 NCBI Official Symbol    SERPINA1 [Similar Products]
 NCBI Official Synonym Symbols   
PI; A1A; AAT; PI1; A1AT; PRO2275; alpha1AT
[Similar Products]
 NCBI Protein Information    alpha-1-antitrypsin
 UniProt Protein Name    Alpha-1-antitrypsin
 UniProt Synonym Protein Names   
Alpha-1 protease inhibitor; Alpha-1-antiproteinase; Serpin A1
 UniProt Gene Name    SERPINA1 [Similar Products]
 UniProt Synonym Gene Names    AAT; PI; SPAAT [Similar Products]
 UniProt Entry Name    A1AT_HUMAN
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 NCBI Summary for a1AT    The protein encoded by this gene is secreted and is a serine protease inhibitor whose targets include elastase, plasmin, thrombin, trypsin, chymotrypsin, and plasminogen activator. Defects in this gene can cause emphysema or liver disease. Several transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
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 UniProt Comments for a1AT    SERPINA1: Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin. Defects in SERPINA1 are the cause of alpha-1-antitrypsin deficiency (A1ATD). A disorder whose most common manifestation is emphysema, which becomes evident by the third to fourth decade. A less common manifestation of the deficiency is liver disease, which occurs in children and adults, and may result in cirrhosis and liver failure. Environmental factors, particularly cigarette smoking, greatly increase the risk of emphysema at an earlier age. Belongs to the serpin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide; Inhibitor

Chromosomal Location of Human Ortholog: 14q32.1

Cellular Component: Golgi apparatus; proteinaceous extracellular matrix; extracellular space; endoplasmic reticulum; extracellular region

Molecular Function: serine-type endopeptidase inhibitor activity; identical protein binding; protein binding; protease binding; glycoprotein binding

Biological Process: platelet activation; response to chromate; platelet degranulation; response to triglyceride; response to cytokine stimulus; response to lead ion; response to methanol; response to hypoxia; acute-phase response; response to lipopolysaccharide; blood coagulation; response to estradiol stimulus

Disease: Alpha-1-antitrypsin Deficiency; Pulmonary Disease, Chronic Obstructive
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 Research Articles on a1AT    1. Alpha-1 proteinase inhibitor M358R inhibits exogenous thrombin and is a membrane protein.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with a1AT elisa kitDiseases associated with a1AT elisa kit
 Products by Pathway  Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 FOXA1 Transcription Factor Network Pathway antibodies  FOXA1 Transcription Factor Network Pathway Diagram
 Hemostasis Pathway antibodies  Hemostasis Pathway Diagram
 Platelet Activation, Signaling And Aggregation Pathway antibodies  Platelet Activation, Signaling And Aggregation Pathway Diagram
 Platelet Degranulation Pathway antibodies  Platelet Degranulation Pathway Diagram
 Response To Elevated Platelet Cytosolic Ca2+ Pathway antibodies  Response To Elevated Platelet Cytosolic Ca2+ Pathway Diagram
 Disease Name  Pubmed Publications
 Lung Diseases Antibodies  >131 publications with a1AT and Lung Diseases
 alpha 1-Antitrypsin Deficiency Antibodies  >129 publications with a1AT and alpha 1-Antitrypsin Deficiency
 Liver Diseases Antibodies  >120 publications with a1AT and Liver Diseases
 Pulmonary Disease, Chronic Obstructive Antibodies  >53 publications with a1AT and Pulmonary Disease, Chronic Obstructive
 Inflammation Antibodies  >36 publications with a1AT and Inflammation
 Pulmonary Emphysema Antibodies  >36 publications with a1AT and Pulmonary Emphysema
 Liver Cirrhosis Antibodies  >29 publications with a1AT and Liver Cirrhosis
 Carcinoma, Hepatocellular Antibodies  >21 publications with a1AT and Carcinoma, Hepatocellular
 Adenocarcinoma Antibodies  >18 publications with a1AT and Adenocarcinoma
 Hepatitis Antibodies  >18 publications with a1AT and Hepatitis
Organs/Tissues associated with a1AT elisa kit
 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >109 publications with a1AT and Liver
 Blood Antibodies  >102 publications with a1AT and Blood
 Lung Antibodies  >71 publications with a1AT and Lung
 Skin Antibodies  >14 publications with a1AT and Skin
 Vascular Antibodies  >11 publications with a1AT and Vascular
 Heart Antibodies  >11 publications with a1AT and Heart
 Kidney Antibodies  >10 publications with a1AT and Kidney
 Pancreas Antibodies  >10 publications with a1AT and Pancreas
 Bone Antibodies  >9 publications with a1AT and Bone
 Muscle Antibodies  >8 publications with a1AT and Muscle
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