View our list of available Coronavirus (COVID-19, SARS-CoV-2, 2019-nCOV) antibodies, recombinant proteins, ELISA Kits... Options Full Datasheet Printer Friendly Datasheet Prepare for Printing Full Datasheet Printer Friendly Datasheet Prepare for Printing Have Questions? Ask Us! Request A Quote Today! Add To Compare List AMN elisa kit :: Rat Amnionless (AMN) ELISA Kit Catalog # MBS2024816 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment) Unit / Price 24-Strip-Wells / $390 +1 FREE 8GB USB 48-Strip-Wells / $590 +1 FREE 8GB USB 96-Strip-Wells / $785 +1 FREE 8GB USB 5x96-Strip-Wells / $3,175 +3 FREE 8GB USB 10x96-Strip-Wells / $5,800 +7 FREE 8GB USB Product Name Product Info Accession #s Product Desc Diseases/Tissues/Pathways Applications References Product Name Amnionless (AMN), ELISA Kit Full Product Name Rat Amnionless (AMN) ELISA Kit Product Gene Name AMN elisa kit[Similar Products] Research Use Only For Research Use Only. Not for use in diagnostic procedures. TOP Sample Manual Insert Download Sample PDF Manual View Sample PDF Manual OMIM 261100 Species Reactivity Rat Specificity This assay has high sensitivity and excellent specificity for detection of Amnionless (AMN).No significant cross-reactivity or interference between Amnionless (AMN) and analogues was observed. Assay Type Double-antibody Sandwich Samples Tissue homogenates, Cell lysates and other biological fluids Detection Range 0.625-40ng/mL Sensitivity 0.214ng/mL Application Enzyme-linked immunosorbent assay for Antigen Detection. Intra-assay Precision (Precision within an assay) 3 samples with low, middle and high level Amnionless (AMN) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) 3 samples with low, middle and high level Amnionless (AMN) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay CV<10% Inter-Assay CV<12% Preparation and Storage The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end. ISO Certification Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory. Supply Chain Verification Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire. Product Note Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details. Other Notes Small volumes of AMN elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply. Searchable Terms for AMN purchase MBS2024816 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Amnionless (AMN) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing AMN. The ELISA analytical biochemical technique of the MBS2024816 kit is based on AMN antibody-AMN antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect AMN antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, AMN. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%). TOP Related Product Information for AMN elisa kit The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Amnionless (AMN). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Amnionless (AMN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Amnionless (AMN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Amnionless (AMN) in the samples is then determined by comparing the O.D. of the samples to the standard curve. Product Categories/Family for AMN elisa kit Reproductive science; Developmental science; Gastroenterology Typical Testing Data/Standard Curve (for reference only) of AMN elisa kit TOP Sample Manual Insert of MBS2024816. NCBI/Uniprot data below describe general gene information for AMN. It may not necessarily be applicable to this product. NCBI GI # 13507259 NCBI GeneID 81693 NCBI Accession # AAK28532.1 [Other Products] UniProt Secondary Accession # Q6UX83[Other Products] UniProt Related Accession # Q9BXJ7[Other Products] Molecular Weight 47,754 Da TOP NCBI Official Full Name amnionless NCBI Official Synonym Full Names amnion associated transmembrane protein NCBI Official Symbol AMN [Similar Products] NCBI Official Synonym Symbols PRO1028; amnionless [Similar Products] NCBI Protein Information protein amnionless UniProt Protein Name Protein amnionless Protein Family AMP nucleosidase UniProt Gene Name AMN [Similar Products] UniProt Entry Name AMNLS_HUMAN TOP NCBI Summary for AMN The protein encoded by this gene is a type I transmembrane protein. It is thought to modulate bone morphogenetic protein (BMP) receptor function by serving as an accessory or coreceptor, and thus facilitates or hinders BMP binding. It is known that the mouse AMN gene is expressed in the extraembryonic visceral endoderm layer during gastrulation, but it is found to be mutated in amnionless mouse. The encoded protein has sequence similarity to short gastrulation (Sog) and procollagen IIA proteins in Drosophila. [provided by RefSeq, Jul 2008] TOP UniProt Comments for AMN AMN: Necessary for efficient absorption of vitamin B12. May direct the production of trunk mesoderm during development by modulating a bone morphogenetic protein (BMP) signaling pathway in the underlying visceral endoderm. Defects in AMN are a cause of recessive hereditary megaloblastic anemia 1 (RH-MGA1); also known as MGA1 Norwegian type or Imerslund-Grasbeck syndrome (I-GS). RH-MGA1 is due to selective malabsorption of vitamin B12. Defects in vitamin B12 absorption lead to impaired function of thymidine synthase. As a consequence DNA synthesis is interrupted. Rapidly dividing cells involved in erythropoiesis are particularly affected. 1 isoforms of the human protein are produced by alternative promoter.Protein type: Membrane protein, integralChromosomal Location of Human Ortholog: 14q32.3Cellular Component: extracellular space; endocytic vesicle; apical plasma membrane; integral to membrane; plasma membrane; endosome membraneMolecular Function: receptor bindingBiological Process: receptor-mediated endocytosis; vitamin metabolic process; cobalamin metabolic process; multicellular organismal development; cobalamin transport; Golgi to plasma membrane protein transport; lipoprotein metabolic process; excretion; water-soluble vitamin metabolic processDisease: Megaloblastic Anemia 1 Research Articles on AMN 1. heterozygous mutations in AMN in a family from the United Kingdom with clinical features of Imerslund-Grasbeck Syndrome TOP Precautions All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines. Disclaimer While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information. TOP Products associated with AMN elisa kit Reference Product PubMed Publications ABCD3 elisa kit >2 publications with AMN and ABCD3 Pathways associated with AMN elisa kit Products by Pathway Pathway Diagram Cobalamin (Cbl, Vitamin B12) Transport And Metabolism Pathway antibodies Cobalamin (Cbl, Vitamin B12) Transport And Metabolism Pathway Diagram Defective AMN Causes Hereditary Megaloblastic Anemia 1 Pathway antibodies Defective AMN Causes Hereditary Megaloblastic Anemia 1 Pathway Diagram Defective BTD Causes Biotidinase Deficiency Pathway antibodies Defective BTD Causes Biotidinase Deficiency Pathway Diagram Defective CD320 Causes Methylmalonic Aciduria Pathway antibodies Defective CD320 Causes Methylmalonic Aciduria Pathway Diagram Defective CUBN Causes Hereditary Megaloblastic Anemia 1 Pathway antibodies Defective CUBN Causes Hereditary Megaloblastic Anemia 1 Pathway Diagram Defective GIF Causes Intrinsic Factor Deficiency Pathway antibodies Defective GIF Causes Intrinsic Factor Deficiency Pathway Diagram Defective HLCS Causes Multiple Carboxylase Deficiency Pathway antibodies Defective HLCS Causes Multiple Carboxylase Deficiency Pathway Diagram Defective LMBRD1 Causes Methylmalonic Aciduria And Homocystinuria Type CblF Pathway antibodies Defective LMBRD1 Causes Methylmalonic Aciduria And Homocystinuria Type CblF Pathway Diagram Defective MMAA Causes Methylmalonic Aciduria Type CblA Pathway antibodies Defective MMAA Causes Methylmalonic Aciduria Type CblA Pathway Diagram Defective MMAB Causes Methylmalonic Aciduria Type CblB Pathway antibodies Defective MMAB Causes Methylmalonic Aciduria Type CblB Pathway Diagram Diseases associated with AMN elisa kit Disease Name Pubmed Publications Nervous System Diseases Antibodies >196 publications with AMN and Nervous System Diseases Kidney Diseases Antibodies >33 publications with AMN and Kidney Diseases Necrosis Antibodies >25 publications with AMN and Necrosis Hypertrophy Antibodies >25 publications with AMN and Hypertrophy MEGALOBLASTIC ANEMIA 1 Antibodies >25 publications with AMN and MEGALOBLASTIC ANEMIA 1 Spinal Cord Diseases Antibodies >23 publications with AMN and Spinal Cord Diseases Congenital Abnormalities Antibodies >23 publications with AMN and Congenital Abnormalities Peripheral Nervous System Diseases Antibodies >21 publications with AMN and Peripheral Nervous System Diseases Disease Models, Animal Antibodies >20 publications with AMN and Disease Models, Animal Memory Disorders Antibodies >12 publications with AMN and Memory Disorders Organs/Tissues associated with AMN elisa kit Organ/Tissue Name Pubmed Publications Heart Antibodies >220 publications with AMN and Heart Muscle Antibodies >96 publications with AMN and Muscle Kidney Antibodies >43 publications with AMN and Kidney Intestine Antibodies >19 publications with AMN and Intestine Testis Antibodies >11 publications with AMN and Testis Lung Antibodies >8 publications with AMN and Lung Stomach Antibodies >4 publications with AMN and Stomach Pancreas Antibodies >3 publications with AMN and Pancreas Lymph Node Antibodies >2 publications with AMN and Lymph Node TOP © 2006-24 MyBioSource.com. 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