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ASD elisa kit :: Rat Androstenedione (ASD) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS260899
Typical Standard Curve/Testing Data
Unit / Price
48-Strip-Wells  /  $275 +1 FREE 8GB USB
96-Strip-Wells  /  $435 +1 FREE 8GB USB
5x96-Strip-Wells  /  $1,755 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,150 +3 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Androstenedione (ASD), ELISA Kit

★Popular Item★
 Also Known As   

Rat Androstenedione (ASD) ELISA Kit

 Product Gene Name   

ASD elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    gene 614279
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 Species Reactivity    Rat
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 Specificity    No cross-reaction with other factors.
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 Samples    Serum, plasma or cell culture supernatant
 Assay Type    Quantitative Sandwich
 Detection Range    10 ng/ml-0.156 ng/ml
 Sensitivity    Up to 0.05ng/ml.
 Intra-assay Precision    <= 8%
 Inter-assay Precision    <= 12%
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of ASD elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for ASD purchase    MBS260899 is a ready-to-use microwell, strip plate Quantitative Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Androstenedione (ASD) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 10 ng/ml-0.156 ng/ml in biological research samples containing ASD, with an estimated sensitivity of Up to 0.05ng/ml. The ELISA analytical biochemical technique of the MBS260899 kit is based on ASD antibody-ASD antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ASD antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ASD. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma or cell culture supernatant. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for ASD elisa kit

   Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Rat ASD monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
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 Typical Standard Curve/Testing Data of ASD elisa kit    ASD elisa kit Typical Standard Curve/Testing Data image
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Sample Manual Insert of MBS260899. Click to request current manual
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NCBI/Uniprot data below describe general gene information for ASD. It may not necessarily be applicable to this product.
 NCBI GI #    20532374
 NCBI GeneID    1646
 NCBI Accession #    P52895.3 [Other Products]
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 UniProt Secondary Accession #    Q14133; Q5SR16; Q7M4N1; Q96A71; A8K2N9; B4DKR9 [Other Products]
 UniProt Related Accession #    P52895 [Other Products]
 Molecular Weight    15,748 Da
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 NCBI Official Full Name    Aldo-keto reductase family 1 member C2
 NCBI Official Synonym Full Names    aldo-keto reductase family 1, member C2
 NCBI Official Symbol    AKR1C2 [Similar Products]
 NCBI Official Synonym Symbols   
DD; DD2; TDD; BABP; DDH2; HBAB; HAKRD; MCDR2; SRXY8; AKR1C-pseudo
[Similar Products]
 NCBI Protein Information    aldo-keto reductase family 1 member C2; DD-2; DD/BABP; 3-alpha-HSD3; pseudo-chlordecone reductase; type II dihydrodiol dehydrogenase; chlordecone reductase homolog HAKRD; testicular 17,20-desmolase deficiency; trans-1,2-dihydrobenzene-1,2-diol dehydrogenase; dihydrodiol dehydrogenase 2; bile acid binding protein; 3-alpha hydroxysteroid dehydrogenase, type III
 UniProt Protein Name    Aldo-keto reductase family 1 member C2
 UniProt Synonym Protein Names   
3-alpha-HSD3; Chlordecone reductase homolog HAKRD; Dihydrodiol dehydrogenase 2; DD-2; DD2; Dihydrodiol dehydrogenase/bile acid-binding protein; DD/BABP; Trans-1,2-dihydrobenzene-1,2-diol dehydrogenase (EC:1.3.1.20); Type III 3-alpha-hydroxysteroid dehydrogenase (EC:1.1.1.357)
 Protein Family    Aspartate-semialdehyde dehydrogenase
 UniProt Gene Name    AKR1C2 [Similar Products]
 UniProt Synonym Gene Names    DDH2; DD-2; DD2; DD/BABP [Similar Products]
 UniProt Entry Name    AK1C2_HUMAN
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 NCBI Summary for ASD    This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols using NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme binds bile acid with high affinity, and shows minimal 3-alpha-hydroxysteroid dehydrogenase activity. This gene shares high sequence identity with three other gene members and is clustered with those three genes at chromosome 10p15-p14. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]
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 UniProt Comments for ASD    AKR1C2: Works in concert with the 5-alpha/5-beta-steroid reductases to convert steroid hormones into the 3-alpha/5-alpha and 3-alpha/5-beta-tetrahydrosteroids. Catalyzes the inactivation of the most potent androgen 5-alpha-dihydrotestosterone (5-alpha- DHT) to 5-alpha-androstane-3-alpha,17-beta-diol (3-alpha-diol). Has a high bile-binding ability. Defects in AKR1C2 are a cause of 46,XY sex reversal type 8 (SRXY8). A disorder of sex development. Affected individuals have a 46,XY karyotype but present as phenotypically normal females. Belongs to the aldo/keto reductase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Xenobiotic Metabolism - metabolism by cytochrome P450; EC 1.3.1.20; EC 1.1.1.357; Oxidoreductase

Chromosomal Location of Human Ortholog: 10p15-p14

Cellular Component: cytoplasm

Molecular Function: oxidoreductase activity, acting on NADH or NADPH, quinone or similar compound as acceptor; ketosteroid monooxygenase activity; trans-1,2-dihydrobenzene-1,2-diol dehydrogenase activity; carboxylic acid binding; bile acid binding; aldehyde reductase activity; phenanthrene 9,10-monooxygenase activity

Biological Process: steroid metabolic process; G-protein coupled receptor protein signaling pathway; positive regulation of protein kinase B signaling cascade; epithelial cell differentiation; digestion; positive regulation of cell proliferation; progesterone metabolic process; prostaglandin metabolic process

Disease: 46,xy Sex Reversal 8
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 Research Articles on ASD    1. Significantly higher levels of SRD5A1, AKR1C2, AKR1C3, and HSD17B10 mRNA were however found in bone metastases than in non-malignant and/or malignant prostate tissue
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with ASD elisa kitPathways associated with ASD elisa kit
 Reference Product  PubMed Publications
 AKR1C1 elisa kit  >90 publications with ASD and AKR1C1
 AKR1C3 elisa kit  >81 publications with ASD and AKR1C3
 SRD5A2 elisa kit  >15 publications with ASD and SRD5A2
 SRD5A1 elisa kit  >12 publications with ASD and SRD5A1
 HSD17B6 elisa kit  >1 publications with ASD and HSD17B6
 Products by Pathway  Pathway Diagram
 Benzo(a)pyrene Metabolism Pathway antibodies  Benzo(a)pyrene Metabolism Pathway Diagram
 Bile Acid And Bile Salt Metabolism Pathway antibodies  Bile Acid And Bile Salt Metabolism Pathway Diagram
 Chemical Carcinogenesis Pathway antibodies  Chemical Carcinogenesis Pathway Diagram
 Chemical Carcinogenesis Pathway antibodies  Chemical Carcinogenesis Pathway Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Lipids And Lipoproteins Pathway antibodies  Metabolism Of Lipids And Lipoproteins Pathway Diagram
 Metabolism Of Xenobiotics By Cytochrome P450 Pathway antibodies  Metabolism Of Xenobiotics By Cytochrome P450 Pathway Diagram
 Metabolism Of Xenobiotics By Cytochrome P450 Pathway antibodies  Metabolism Of Xenobiotics By Cytochrome P450 Pathway Diagram
 Steroid Hormone Biosynthesis Pathway antibodies  Steroid Hormone Biosynthesis Pathway Diagram
 Steroid Hormone Biosynthesis Pathway antibodies  Steroid Hormone Biosynthesis Pathway Diagram
Diseases associated with ASD elisa kitOrgans/Tissues associated with ASD elisa kit
 Disease Name  Pubmed Publications
 Carcinoma Antibodies  >27 publications with ASD and Carcinoma
 Adenocarcinoma Antibodies  >20 publications with ASD and Adenocarcinoma
 Breast Neoplasms Antibodies  >17 publications with ASD and Breast Neoplasms
 Liver Neoplasms Antibodies  >10 publications with ASD and Liver Neoplasms
 Hyperplasia Antibodies  >6 publications with ASD and Hyperplasia
 Fatty Liver Antibodies  >6 publications with ASD and Fatty Liver
 Lung Neoplasms Antibodies  >6 publications with ASD and Lung Neoplasms
 Lung Diseases Antibodies  >5 publications with ASD and Lung Diseases
 Endometriosis Antibodies  >5 publications with ASD and Endometriosis
 Inflammation Antibodies  >4 publications with ASD and Inflammation
 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >102 publications with ASD and Liver
 Intestine Antibodies  >60 publications with ASD and Intestine
 Blood Antibodies  >20 publications with ASD and Blood
 Kidney Antibodies  >15 publications with ASD and Kidney
 Skin Antibodies  >14 publications with ASD and Skin
 Lung Antibodies  >13 publications with ASD and Lung
 Uterus Antibodies  >10 publications with ASD and Uterus
 Adipose Tissue Antibodies  >10 publications with ASD and Adipose Tissue
 Connective Tissue Antibodies  >10 publications with ASD and Connective Tissue
 Ovary Antibodies  >9 publications with ASD and Ovary
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