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AT1R-AA elisa kit :: Rat Angiotensin 2 Receptor 1 Autoantibodies ELISA Kit

Scan QR to view Datasheet Catalog #    MBS731165
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $455 +1 FREE 8GB USB
96-Strip-Wells  /  $660 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,925 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,490 +6 FREE 8GB USB
 
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 Product Name   

Angiotensin 2 Receptor 1 Autoantibodies (AT1R-AA), ELISA Kit

★Popular Item★
 Also Known As   

Rat Angiotensin 2 Receptor 1 Autoantibodies ELISA Kit

 Product Gene Name   

AT1R-AA elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 Species Reactivity    Rat
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 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Assay Type    Competitive
 Detection Range    0.5-10 ng/mL
 Sensitivity    0.1 ng/mL
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of AT1R-AA elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for AT1R-AA purchase    MBS731165 is a ready-to-use microwell, strip plate Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Angiotensin 2 Receptor 1 Autoantibodies (AT1R-AA) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.5-10 ng/mL in biological research samples containing AT1R-AA, with an estimated sensitivity of 0.1 ng/mL. The ELISA analytical biochemical technique of the MBS731165 kit is based on AT1R-AA antibody-AT1R-AA antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect AT1R-AA antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, AT1R-AA. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%). The human angiotensin protein and receptor family are key blood pressure regulators. Autoantibodies IgG type, blockers of receptor signaling, are an endogenous BP reducing mechanism which is easily studied in rat model. Assay of these auto antibodies in cell or serum samples is accomplished with a specific angiotensin ii receptor (angiotensin 2 receptor ) 1 AT1R ELISA kit. Building quality anti 1 autoantibodies ELISA kits requires attention to details and specific monoclonal polyclonal products. Our assay receptor 1 autoantibodies IgG1 kits exceed your expectations, with anti antiotensin ii receptor type 1 antibody 96 tests plate or 48 wells strips, wash solution, standard curve product, all meeting the highest reagents products standards. We also offer related ELISA assay kit ankyrin or ELISA kit angiotensin or ELISA kit angiopoietin and a separate anti angiotensin ii antibody ELISA assay. With our current promotional pricing for assay kits, you can afford to repeat results.
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Related Product Information for AT1R-AA elisa kit

   For samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

INTENDED USE This AT1R-AA ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat AT1R-AA. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

PRINCIPLE OF THE ASSAY AT1R-AA ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with Angiotensin 2 Receptor 1 antigen. Standards or samples are then added to the microtiter plate wells and AT1R-AA if present, will bind to the antigen pre-coated wells. In order to quantitatively determine the amount of AT1R-AA present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated Angiotensin 2 Receptor 1 antigen is added to each well to “sandwich” the AT1R-AA immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain AT1R-AA and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AT1R-AA concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for AT1R-AA elisa kit    Immunology
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 Typical Testing Data/Standard Curve (for reference only) of AT1R-AA elisa kit    AT1R-AA elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS731165. Click to request current manual
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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