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Ace elisa kit :: Rat Angiotensin-converting enzyme ELISA Kit

Scan QR to view Datasheet Catalog #    MBS764783
Typical Standard Curve/Testing Data
Unit / Price
48-Strip-Wells  /  $295 +1 FREE 8GB USB
96-Strip-Wells  /  $390 +1 FREE 8GB USB
5x96-Strip-Wells  /  $1,615 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,015 +3 FREE 8GB USB
15x96-Strip-Wells  /  $4,285 +5 FREE 8GB USB
25x96-Strip-Wells  /  $6,715 +8 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Angiotensin-converting enzyme (Ace), ELISA Kit

★Popular Item★
 Also Known As   

Rat Angiotensin-converting enzyme ELISA Kit

 Product Synonym Names    ACE/CD143/DCP1/Kininase II/ACE1angiotensin converting enzyme; somatic isoform/angiotensin I converting enzyme (peptidyl-dipeptidase A) 1/carboxycathepsin/CD143/CD143 antigen/DCP/DCP1angiotensin-converting enzyme/dipeptidyl carboxypeptidase 1/Dipeptidyl carboxypeptidase I/ Kininase II/MVCD3/peptidase P/testicular ECA
 Product Gene Name   

Ace elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS764783 COA    COA PDF
 OMIM    106180
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 Species Reactivity    Rat
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Ace. No significant cross-reactivity or interference between Ace and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates and other biological fluids
 Assay Type    Quantitative Sandwich
 Detection Range    0.313-20ng/ml
 Sensitivity    <0.188ng/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ace were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
 Inter-assay Precision    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ace were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
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 Preparation and Storage    Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of Ace elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for Ace purchase    MBS764783 is a ready-to-use microwell, strip plate Quantitative Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Angiotensin-converting enzyme (Ace) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.313-20ng/ml in biological research samples containing Ace, with an estimated sensitivity of <0.188ng/ml. The ELISA analytical biochemical technique of the MBS764783 kit is based on Ace antibody-Ace antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Ace antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Ace. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for Ace elisa kit

   Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- Ace antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- Ace antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Ace amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Ace can be calculated.
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 Typical Standard Curve/Testing Data of Ace elisa kit    Ace elisa kit Typical Standard Curve/Testing Data image
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Sample Manual Insert of MBS764783. Click to request current manual
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NCBI/Uniprot data below describe general gene information for Ace. It may not necessarily be applicable to this product.
 NCBI GI #    338667
 NCBI GeneID    1636
 NCBI Accession #    AAA60611.1 [Other Products]
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 UniProt Secondary Accession #    P22966; Q53YX9; Q59GY8; Q7M4L4; B0LPF0; B4DXI3; E7EU16 [Other Products]
 UniProt Related Accession #    P12821 [Other Products]
 Molecular Weight    78,694 Da
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 NCBI Official Full Name    angiotensin converting enzyme (EC 3.4.15.1)
 NCBI Official Synonym Full Names    angiotensin I converting enzyme
 NCBI Official Symbol    ACE [Similar Products]
 NCBI Official Synonym Symbols   
DCP; ICH; ACE1; DCP1; CD143; MVCD3
[Similar Products]
 NCBI Protein Information    angiotensin-converting enzyme
 UniProt Protein Name    Angiotensin-converting enzyme
 UniProt Synonym Protein Names   
Dipeptidyl carboxypeptidase I; Kininase II; CD_antigen: CD143
 Protein Family    Acetylcholinesterase
 UniProt Gene Name    ACE [Similar Products]
 UniProt Synonym Gene Names    DCP; DCP1; ACE [Similar Products]
 UniProt Entry Name    ACE_HUMAN
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 NCBI Summary for Ace    This gene encodes an enzyme involved in catalyzing the conversion of angiotensin I into a physiologically active peptide angiotensin II. Angiotensin II is a potent vasopressor and aldosterone-stimulating peptide that controls blood pressure and fluid-electrolyte balance. This enzyme plays a key role in the renin-angiotensin system. Many studies have associated the presence or absence of a 287 bp Alu repeat element in this gene with the levels of circulating enzyme or cardiovascular pathophysiologies. Multiple alternatively spliced transcript variants encoding different isoforms have been identified, and two most abundant spliced variants encode the somatic form and the testicular form, respectively, that are equally active. [provided by RefSeq, May 2010]
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 UniProt Comments for Ace    ACE: Converts angiotensin I to angiotensin II by release of the terminal His-Leu, this results in an increase of the vasoconstrictor activity of angiotensin. Also able to inactivate bradykinin, a potent vasodilator. Has also a glycosidase activity which releases GPI-anchored proteins from the membrane by cleaving the mannose linkage in the GPI moiety. Genetic variations in ACE may be a cause of susceptibility to ischemic stroke (ISCHSTR); also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors. Defects in ACE are a cause of renal tubular dysgenesis (RTD). RTD is an autosomal recessive severe disorder of renal tubular development characterized by persistent fetal anuria and perinatal death, probably due to pulmonary hypoplasia from early-onset oligohydramnios (the Potter phenotype). Genetic variations in ACE are associated with susceptibility to microvascular complications of diabetes type 3 (MVCD3). These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new- onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. Defects in ACE are a cause of susceptibility to intracerebral hemorrhage (ICH). A pathological condition characterized by bleeding into one or both cerebral hemispheres including the basal ganglia and the cerebral cortex. It is often associated with hypertension and craniocerebral trauma. Intracerebral bleeding is a common cause of stroke. Belongs to the peptidase M2 family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; EC 3.4.15.1; Protease

Chromosomal Location of Human Ortholog: 17q23.3

Cellular Component: endosome; external side of plasma membrane; extracellular region; extracellular space; integral to membrane; lysosome; plasma membrane

Molecular Function: actin binding; bradykinin receptor binding; carboxypeptidase activity; chloride ion binding; drug binding; endopeptidase activity; exopeptidase activity; metallopeptidase activity; mitogen-activated protein kinase binding; mitogen-activated protein kinase kinase binding; peptidyl-dipeptidase activity; protein binding; tripeptidyl-peptidase activity; zinc ion binding

Biological Process: angiotensin catabolic process in blood; angiotensin maturation; angiotensin mediated regulation of renal output; antigen processing and presentation of peptide antigen via MHC class I; arachidonic acid secretion; beta-amyloid metabolic process; blood vessel remodeling; cellular protein metabolic process; heart contraction; hormone catabolic process; kidney development; mononuclear cell proliferation; neutrophil mediated immunity; peptide catabolic process; regulation of angiotensin metabolic process; regulation of blood pressure; regulation of smooth muscle cell migration; regulation of systemic arterial blood pressure by renin-angiotensin; regulation of vasoconstriction; regulation of vasodilation; spermatogenesis

Disease: Alzheimer Disease; Hemorrhage, Intracerebral, Susceptibility To; Microvascular Complications Of Diabetes, Susceptibility To, 3; Renal Tubular Dysgenesis
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 Research Articles on Ace    1. There is an association between the angiotensin-converting enzyme gene insertion/deletion polymorphism and susceptibility to systemic lupus erythematosus in an Indian population.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with Ace elisa kitPathways associated with Ace elisa kit
 Reference Product  PubMed Publications
 ACE2 elisa kit  >562 publications with Ace and ACE2
 AGT elisa kit  >494 publications with Ace and AGT
 BDKRB2 elisa kit  >342 publications with Ace and BDKRB2
 NOS3 elisa kit  >172 publications with Ace and NOS3
 REN elisa kit  >168 publications with Ace and REN
 ATP6AP2 elisa kit  >11 publications with Ace and ATP6AP2
 TFPI elisa kit  >10 publications with Ace and TFPI
 MDK elisa kit  >3 publications with Ace and MDK
 KNG1 elisa kit  >2 publications with Ace and KNG1
 SOD3 elisa kit  >1 publications with Ace and SOD3
 Products by Pathway  Pathway Diagram
 ACE Inhibitor Pathway antibodies  ACE Inhibitor Pathway Diagram
 Chagas Disease (American Trypanosomiasis) Pathway antibodies  Chagas Disease (American Trypanosomiasis) Pathway Diagram
 Chagas Disease (American Trypanosomiasis) Pathway antibodies  Chagas Disease (American Trypanosomiasis) Pathway Diagram
 Hypertrophic Cardiomyopathy (HCM) Pathway antibodies  Hypertrophic Cardiomyopathy (HCM) Pathway Diagram
 Hypertrophic Cardiomyopathy (HCM) Pathway antibodies  Hypertrophic Cardiomyopathy (HCM) Pathway Diagram
 Metabolism Of Angiotensinogen To Angiotensins Pathway antibodies  Metabolism Of Angiotensinogen To Angiotensins Pathway Diagram
 Metabolism Of Proteins Pathway antibodies  Metabolism Of Proteins Pathway Diagram
 Peptide Hormone Metabolism Pathway antibodies  Peptide Hormone Metabolism Pathway Diagram
 Renin Secretion Pathway antibodies  Renin Secretion Pathway Diagram
 Renin Secretion Pathway antibodies  Renin Secretion Pathway Diagram
Diseases associated with Ace elisa kitOrgans/Tissues associated with Ace elisa kit
 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >14240 publications with Ace and Cardiovascular Diseases
 Hypertension Antibodies  >9242 publications with Ace and Hypertension
 Heart Failure Antibodies  >4777 publications with Ace and Heart Failure
 Kidney Diseases Antibodies  >3598 publications with Ace and Kidney Diseases
 Myocardial Infarction Antibodies  >2631 publications with Ace and Myocardial Infarction
 Coronary Disease Antibodies  >2267 publications with Ace and Coronary Disease
 Stroke Antibodies  >1485 publications with Ace and Stroke
 Renal Insufficiency Antibodies  >1392 publications with Ace and Renal Insufficiency
 Respiratory Tract Diseases Antibodies  >1358 publications with Ace and Respiratory Tract Diseases
 Coronary Artery Disease Antibodies  >1283 publications with Ace and Coronary Artery Disease
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >12688 publications with Ace and Blood
 Heart Antibodies  >8602 publications with Ace and Heart
 Vascular Antibodies  >4748 publications with Ace and Vascular
 Kidney Antibodies  >4428 publications with Ace and Kidney
 Muscle Antibodies  >2227 publications with Ace and Muscle
 Lung Antibodies  >1651 publications with Ace and Lung
 Brain Antibodies  >1394 publications with Ace and Brain
 Intestine Antibodies  >292 publications with Ace and Intestine
 Eye Antibodies  >254 publications with Ace and Eye
 Testis Antibodies  >231 publications with Ace and Testis
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