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C1q elisa kit :: Rat Anti-Complement 1q Antibody ELISA Kit

Scan QR to view Datasheet Catalog #    MBS722996
Typical Standard Curve/Testing Data
Unit / Price
48-Strip-Wells  /  $470 +1 FREE 8GB USB
96-Strip-Wells  /  $675 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,925 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,490 +6 FREE 8GB USB
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 Product Name   

Anti-Complement 1q Antibody (C1q), ELISA Kit

★Popular Item★
 Also Known As   

Rat Anti-Complement 1q Antibody ELISA Kit

 Product Gene Name   

C1q elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    608752
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 Species Reactivity    Rat
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 Specificity    This assay has high sensitivity and excellent specificity for detection of C1QAb. No significant cross-reactivity or interference between C1QAb and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between C1QAb and all the analogues, therefore, cross reaction may still exist in some cases.
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 Samples    Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
 Assay Type    Quantitative Competitive
 Sensitivity    1.0 ng/mL.
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of C1q elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for C1q purchase    MBS722996 is a ready-to-use microwell, strip plate Quantitative Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Anti-Complement 1q Antibody (C1q) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing C1q, with an estimated sensitivity of 1.0 ng/mL. The ELISA analytical biochemical technique of the MBS722996 kit is based on C1q antibody-C1q antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect C1q antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, C1q. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for C1q elisa kit

   Intended Uses: This C1QAb ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat C1QAb. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: C1QAb ELISA kit applies the competitive enzyme immunoassay technique utilizing Complement 1Q antigen and an C1QAb-HRP conjugate. The assay sample and buffer are incubated together with C1QAb-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the C1QAb concentration since C1QAb from samples and C1QAb-HRP conjugate compete for the Complement 1Q antigen binding site. Since the number of sites is limited, as more sites are occupied by C1QAb from the sample, fewer sites are left to bind C1QAb-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The C1QAb concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for C1q elisa kit    Immunology
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 Typical Standard Curve/Testing Data of C1q elisa kit    C1q elisa kit Typical Standard Curve/Testing Data image
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Sample Manual Insert of MBS722996. Click to request current manual
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NCBI/Uniprot data below describe general gene information for C1q. It may not necessarily be applicable to this product.
 NCBI GI #    14149712
 NCBI GeneID    114902
 NCBI Accession #    NP_056460.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_015645.4 [Other Products]
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 UniProt Secondary Accession #    Q335M2; Q8N6P2; Q9UFX4; A6NDD3; B0YJ35 [Other Products]
 UniProt Related Accession #    Q9BXJ0 [Other Products]
 Molecular Weight    25,298 Da
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 NCBI Official Full Name    complement C1q tumor necrosis factor-related protein 5
 NCBI Official Synonym Full Names    C1q and tumor necrosis factor related protein 5
 NCBI Official Symbol    C1QTNF5 [Similar Products]
 NCBI Official Synonym Symbols   
[Similar Products]
 NCBI Protein Information    complement C1q tumor necrosis factor-related protein 5; myonectin; C1q TNF-alpha-related protein 5
 UniProt Protein Name    Complement C1q tumor necrosis factor-related protein 5
 UniProt Gene Name    C1QTNF5 [Similar Products]
 UniProt Synonym Gene Names    CTRP5; UNQ303/PRO344 [Similar Products]
 UniProt Entry Name    C1QT5_HUMAN
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 NCBI Summary for C1q    This gene encodes a member of a family of proteins that function as components of basement membranes and may play a role in cell adhesion. Mutations in this gene have been associated with late-onset retinal degeneration. The protein may be encoded by either a bicistronic transcript including sequence from the upstream membrane frizzled-related protein gene (MFRP), or by a monocistronic transcript expressed from an internal promoter. [provided by RefSeq, Jun 2013]
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 UniProt Comments for C1q    C1QTNF5: Defects in C1QTNF5 are a cause of late-onset retinal degeneration (LORD). LORD is an autosomal dominant disorder characterized by onset in the fifth to sixth decade with night blindness and punctate yellow-white deposits in the retinal fundus, progressing to severe central and peripheral degeneration, with choroidal neovascularization and chorioretinal atrophy.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 11q23.3

Cellular Component: collagen

Disease: Late-onset Retinal Degeneration
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 Research Articles on C1q    1. CTRP-5 might be a novel adipokine that circulates abundantly in human sera.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with C1q elisa kitDiseases associated with C1q elisa kit
 Reference Product  PubMed Publications
 MFRP elisa kit  >7 publications with C1q and MFRP
 TIMP3 elisa kit  >1 publications with C1q and TIMP3
 Disease Name  Pubmed Publications
 Macular Degeneration Antibodies  >12 publications with C1q and Macular Degeneration
 Necrosis Antibodies  >11 publications with C1q and Necrosis
 Late-Onset Retinal Degeneration Antibodies  >9 publications with C1q and Late-Onset Retinal Degeneration
 Atrophy Antibodies  >5 publications with C1q and Atrophy
 Lens Diseases Antibodies  >4 publications with C1q and Lens Diseases
 Inflammation Antibodies  >3 publications with C1q and Inflammation
 Eye Abnormalities Antibodies  >3 publications with C1q and Eye Abnormalities
 Brain Diseases Antibodies  >1 publications with C1q and Brain Diseases
 Kidney Diseases Antibodies  >1 publications with C1q and Kidney Diseases
 Weight Loss Antibodies  >1 publications with C1q and Weight Loss
Organs/Tissues associated with C1q elisa kit
 Organ/Tissue Name  Pubmed Publications
 Eye Antibodies  >21 publications with C1q and Eye
 Connective Tissue Antibodies  >1 publications with C1q and Connective Tissue
 Skin Antibodies  >1 publications with C1q and Skin
 Vascular Antibodies  >1 publications with C1q and Vascular
 Muscle Antibodies  >1 publications with C1q and Muscle
 Heart Antibodies  >1 publications with C1q and Heart
 Ovary Antibodies  >1 publications with C1q and Ovary
 Placenta Antibodies  >1 publications with C1q and Placenta
 Brain Antibodies  >1 publications with C1q and Brain
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