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GM1 elisa kit :: Rat Anti-Ganglioside M1 Antibody ELISA Kit

Scan QR to view Datasheet Catalog #    MBS726610
Typical Testing Data/Standard Curve
Unit / Price
48-Strip-Wells  /  $470 +1 FREE 8GB USB
96-Strip-Wells  /  $675 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,925 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,490 +6 FREE 8GB USB
 
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 Product Name   

Anti-Ganglioside M1 Antibody (GM1), ELISA Kit

 Also Known As   

Rat Anti-Ganglioside M1 Antibody ELISA Kit

 Product Gene Name   

GM1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Manual Insert    Download PDF Manual View PDF Manual
 Species Reactivity    Rat
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 Specificity    This assay has high sensitivity and excellent specificity for detection of GM1Ab. No significant cross-reactivity or interference between GM1Ab and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between GM1Ab and all the analogues, therefore, cross reaction may still exist in some cases.
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 Samples    Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
 Assay Type    Quantitative Competitive
 Sensitivity    1.0 ng/mL.
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay.
 Other Notes    Small volumes of GM1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for GM1 purchase    MBS726610 is a ready-to-use microwell, strip plate Quantitative Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Anti-Ganglioside M1 Antibody (GM1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing GM1, with an estimated sensitivity of 1.0 ng/mL. The ELISA analytical biochemical technique of the MBS726610 kit is based on GM1 antibody-GM1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect GM1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, GM1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for GM1 elisa kit

   Intended Uses: This GM1Ab ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat GM1Ab. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: GM1Ab ELISA kit applies the competitive enzyme immunoassay technique utilizing Ganglioside M1 antigen and an GM1Ab-HRP conjugate. The assay sample and buffer are incubated together with GM1Ab-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GM1Ab concentration since GM1Ab from samples and GM1Ab-HRP conjugate compete for the Ganglioside M1 antigen binding site. Since the number of sites is limited, as more sites are occupied by GM1Ab from the sample, fewer sites are left to bind GM1Ab-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GM1Ab concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for GM1 elisa kit    Cell Biology
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 Typical Testing Data/Standard Curve of GM1 elisa kit    GM1 elisa kit Typical Testing Data/Standard Curve image
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Sample Manual Insert of MBS726610
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NCBI/Uniprot data below describe general gene information for GM1. It may not necessarily be applicable to this product.
 NCBI GI #    1345820
 NCBI Accession #    P48648.2 [Other Products]
 Molecular Weight    21,410 Da
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 NCBI Official Full Name    Gamma-crystallin M1
 UniProt Protein Name    Gamma-crystallin M1
 UniProt Gene Name    GM1 [Similar Products]
 UniProt Synonym Gene Names    Gamma-M1 [Similar Products]
 UniProt Entry Name    CRGM1_CHIID
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 UniProt Comments for GM1    Crystallins are the dominant structural components of the vertebrate eye lens.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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