Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
Fax 1.858.633.0166    Email 
menu1 Home Products Ordering Company Help menu7
   MyBioSource rightarrow ELISA Kit rightarrow GM1 rightarrow Rat GM1  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Menu separator
Monoclonal Antibody Monoclonal Antibody
Polyclonal Antibody Polyclonal Antibody
Secondary Antibody Secondary Antibody
Antigen Antigen
Biochemical Biochemical
cDNA Clone cDNA Clone
Peptide Peptide
Recombinant/Purified Protein Rec./Purified Protein

Custom ELISA Kit Custom ELISA Kit
Custom Protein Custom Protein
Custom Antibody Custom Antibody
Antibody Matched Pairs Antibody Matched Pairs
Antibody & Corresponding Blocking Peptide Pairs Antibody Peptide Pairs
Phospho-Specific Antibodies Phospho Antibodies
Products by Disease Products by Disease
Products by Pathway Products by Pathway
Products by Tissue Products by Tissue

arrow Advanced Search
arrow Submit Technical Q&A
arrow International Distributors
arrow Contact Us
Our Best Sellers moreseparator
 • Cross-Linked C-Terminal Telopeptides of Type II Collagen (CT... ELISA Kit
 • Wolf-Hirschhorn syndrome candidate 2 (NELFA) Antibody
 • Giardia intestinalis Endoribonuclease Dicer-like (GL50803_1... Recombinant Protein
 • CDKN2A Antibody
 • 3 Nitrotyrosine (3 NT) ELISA Kit
 • PRL Antibody
 • Cysteinyl-tRNA synthetase, cytoplasmic (CARS) ELISA Kit
 • E Coli 0157:H7 Antibody
 • Complement Factor H (CFH) ELISA Kit
 • GroEL Recombinant Protein
 • Glutathione S Transferase Alpha 1 (GSTA1) ELISA Kit
 • Interleukin-15 protein (IL15) Recombinant Protein
 • TCL1A Peptide
 • chromosome 14 open reading frame 149 (C14orf149) ELISA Kit
 downarrow more ...
DatasheetFull DatasheetPrinter Friendly DatasheetPrint This DatasheetAdd to Compare ListHave Questions? Ask UsRequest for a Quotation today

GM1 elisa kit :: Rat Anti-Ganglioside M1 Antibody ELISA Kit

Scan QR to view Datasheet Catalog #    MBS726610
Typical Testing Data/Standard Curve
Unit / Price
48-Strip-Wells  /  $470 +1 FREE 8GB USB
96-Strip-Wells  /  $675 +1 FREE 8GB USB
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Anti-Ganglioside M1 Antibody (GM1), ELISA Kit

 Also Known As   

Rat Anti-Ganglioside M1 Antibody ELISA Kit

 Product Gene Name   

GM1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
Table BarTOPTable Bar
  Manual Insert    Download PDF Manual View PDF Manual
 Species Reactivity    Rat
Section Bar
 Specificity    This assay has high sensitivity and excellent specificity for detection of GM1Ab. No significant cross-reactivity or interference between GM1Ab and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between GM1Ab and all the analogues, therefore, cross reaction may still exist in some cases.
Section Bar
 Samples    Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
 Assay Type    Quantitative Competitive
 Sensitivity    1.0 ng/mL.
Section Bar
 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact technical support.
 Other Notes    Small volumes of GM1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for GM1 purchase    MBS726610 is a ready-to-use microwell, strip plate Quantitative Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Anti-Ganglioside M1 Antibody (GM1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing GM1, with an estimated sensitivity of 1.0 ng/mL. The ELISA analytical biochemical technique of the MBS726610 kit is based on GM1 antibody-GM1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect GM1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, GM1. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions such as Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Table BarTOPTable Bar

Product Description specifically for GM1 elisa kit

   Intended Uses: This GM1Ab ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat GM1Ab. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: GM1Ab ELISA kit applies the competitive enzyme immunoassay technique utilizing Ganglioside M1 antigen and an GM1Ab-HRP conjugate. The assay sample and buffer are incubated together with GM1Ab-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GM1Ab concentration since GM1Ab from samples and GM1Ab-HRP conjugate compete for the Ganglioside M1 antigen binding site. Since the number of sites is limited, as more sites are occupied by GM1Ab from the sample, fewer sites are left to bind GM1Ab-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GM1Ab concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for GM1 elisa kit    Cell Biology
Section Bar
 Typical Testing Data/Standard Curve of GM1 elisa kit    GM1 elisa kit Typical Testing Data/Standard Curve image
Table BarTOPTable Bar
Sample Manual Insert of MBS726610
Table BarTOPTable Bar
NCBI/Uniprot data below describe general gene information for GM1. It may not necessarily be applicable to this product.
 NCBI GI #    1345820
 NCBI Accession #    P48648.2 [Other Products]
 Molecular Weight    21,410 Da
Table BarTOPTable Bar
 NCBI Official Full Name    Gamma-crystallin M1
 UniProt Protein Name    Gamma-crystallin M1
 UniProt Gene Name    GM1 [Similar Products]
 UniProt Synonym Gene Names    Gamma-M1 [Similar Products]
 UniProt Entry Name    CRGM1_CHIID
Section Bar
 UniProt Comments for GM1    Crystallins are the dominant structural components of the vertebrate eye lens.
Table BarTOPTable Bar
 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Table BarTOPTable Bar
Products related to Rat Anti-Ganglioside M1 Antibody ELISA Kit
 Catalog #  Product Name  Catalog #  Product Name
 MBS288514  Human anti-ganglioside M1 antibody ELISA Kit  MBS727387  Rabbit Anti-Ganglioside M1 Antibody ELISA Kit
 MBS752740  Bovine Anti-Ganglioside M1 Antibody ELISA Kit  MBS752768  Goat Anti-Ganglioside M1 Antibody ELISA Kit
 MBS764294  Human Anti-Ganglioside M1 Antibody ELISA Kit  MBS2531635  Human Anti-GM1 (Anti-Ganglioside M1 Antibody) CLIA Kit
 MBS753718  Sheep Anti-Ganglioside M1 Antibody ELISA Kit  MBS726610  Rat Anti-Ganglioside M1 Antibody ELISA Kit
 MBS752975  Monkey Anti-Ganglioside M1 Antibody ELISA Kit  MBS754940  Porcine Anti-Ganglioside M1 Antibody ELISA Kit
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions