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TPO elisa kit :: Rat Anti-Thrombopoietin Antibody (anti-TPO Ab) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS262061
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $290 +1 FREE 8GB USB
96-Strip-Wells  /  $450 +1 FREE 8GB USB
5x96-Strip-Wells  /  $1,755 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,150 +3 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Anti-Thrombopoietin Antibody (anti-TPO Ab), ELISA Kit

★Popular Item★
 Also Known As   

Rat Anti-Thrombopoietin Antibody (anti-TPO Ab) ELISA Kit

 Product Gene Name   

TPO elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    606765
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 Species Reactivity    Rat
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 Specificity    No cross-reaction with other factors.
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 Samples    Serum, plasma or cell culture supernatant and organizations in the natural and recombinant anti-TPO Ab concentration.
 Assay Type    Sandwich
 Detection Range    10 ng/ml-0.156 ng/ml
 Sensitivity    0.05 ng/ml.
 Intra-assay Precision    <= 8%
 Inter-assay Precision    <= 12%
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of TPO elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for TPO purchase    MBS262061 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Anti-Thrombopoietin Antibody (anti-TPO Ab) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 10 ng/ml-0.156 ng/ml in biological research samples containing TPO, with an estimated sensitivity of 0.05 ng/ml. The ELISA analytical biochemical technique of the MBS262061 kit is based on TPO antibody-TPO antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TPO antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, TPO. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma or cell culture supernatant and organizations in the natural and recombinant anti-TPO Ab concentration. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for TPO elisa kit

   Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antigen is Rat anti-TPO Ab antigen.Samples and antigen are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
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 Typical Testing Data/Standard Curve (for reference only) of TPO elisa kit    TPO elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS262061. Click to request current manual
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NCBI/Uniprot data below describe general gene information for TPO. It may not necessarily be applicable to this product.
 NCBI GI #    332078492
 NCBI GeneID    7173
 NCBI Accession #    NP_001193674.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001206745.1 [Other Products]
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 UniProt Secondary Accession #    P09934; P09935; Q8IUL0; Q8NF94; Q8NF95; Q8NF96; Q8NF97; Q8TCI9 [Other Products]
 UniProt Related Accession #    P07202 [Other Products]
 Molecular Weight    92,063 Da
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 NCBI Official Full Name    thyroid peroxidase isoform b
 NCBI Official Synonym Full Names    thyroid peroxidase
 NCBI Official Symbol    TPO [Similar Products]
 NCBI Official Synonym Symbols   
MSA; TPX; TDH2A
[Similar Products]
 NCBI Protein Information    thyroid peroxidase; thyroperoxidase; thyroid microsomal antigen
 UniProt Protein Name    Thyroid peroxidase
 Protein Family    Thyroid peroxidase
 UniProt Gene Name    TPO [Similar Products]
 UniProt Synonym Gene Names    TPO [Similar Products]
 UniProt Entry Name    PERT_HUMAN
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 NCBI Summary for TPO    This gene encodes a membrane-bound glycoprotein. The encoded protein acts as an enzyme and plays a central role in thyroid gland function. The protein functions in the iodination of tyrosine residues in thyroglobulin and phenoxy-ester formation between pairs of iodinated tyrosines to generate the thyroid hormones, thyroxine and triiodothyronine. Mutations in this gene are associated with several disorders of thyroid hormonogenesis, including congenital hypothyroidism, congenital goiter, and thyroid hormone organification defect IIA. Multiple transcript variants encoding distinct isoforms have been identified for this gene, but the full-length nature of some variants has not been determined. [provided by RefSeq, May 2011]
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 UniProt Comments for TPO    TPO: Iodination and coupling of the hormonogenic tyrosines in thyroglobulin to yield the thyroid hormones T(3) and T(4). An alternative splicing in the thyroperoxidase mRNA can cause Graves' disease. Defects in TPO are the cause of thyroid dyshormonogenesis 2A (TDH2A). A disorder due to defective conversion of accumulated iodide to organically bound iodine. The iodide organification defect can be partial or complete. Belongs to the peroxidase family. XPO subfamily. 8 isoforms of the human protein are produced by alternative splicing.

Protein type: Oxidoreductase; Mitochondrial; Amino Acid Metabolism - tyrosine; EC 1.11.1.8; Membrane protein, integral

Chromosomal Location of Human Ortholog: 2p25

Cellular Component: extracellular space; cell surface; mitochondrion; integral to plasma membrane; plasma membrane

Molecular Function: peroxidase activity; heme binding; iodide peroxidase activity; calcium ion binding

Biological Process: embryonic hemopoiesis; hydrogen peroxide catabolic process; thyroid hormone generation; response to oxidative stress; hormone biosynthetic process

Disease: Thyroid Dyshormonogenesis 2a
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 Research Articles on TPO    1. One fourth of type 1 diabetes mellitus pregnant women presented with positive anti-TPO antibodies. However, the presence of anti-TPO antibodies does not seem to be related with worse metabolic control or adverse pregnancy outcome.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with TPO elisa kitDiseases associated with TPO elisa kit
 Products by Pathway  Pathway Diagram
 Amine-derived Hormones Pathway antibodies  Amine-derived Hormones Pathway Diagram
 Autoimmune Thyroid Disease Pathway antibodies  Autoimmune Thyroid Disease Pathway Diagram
 Autoimmune Thyroid Disease Pathway antibodies  Autoimmune Thyroid Disease Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram
 Jak-STAT Signaling Pathway antibodies  Jak-STAT Signaling Pathway Diagram
 Jak-STAT Signaling Pathway antibodies  Jak-STAT Signaling Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram
 Disease Name  Pubmed Publications
 Thyroid Diseases Antibodies  >1228 publications with TPO and Thyroid Diseases
 Goiter Antibodies  >469 publications with TPO and Goiter
 Thyroid Neoplasms Antibodies  >195 publications with TPO and Thyroid Neoplasms
 Blood Coagulation Disorders Antibodies  >132 publications with TPO and Blood Coagulation Disorders
 Liver Diseases Antibodies  >127 publications with TPO and Liver Diseases
 Congenital Hypothyroidism Antibodies  >106 publications with TPO and Congenital Hypothyroidism
 Hepatitis Antibodies  >93 publications with TPO and Hepatitis
 Mental Disorders Antibodies  >92 publications with TPO and Mental Disorders
 Central Nervous System Diseases Antibodies  >83 publications with TPO and Central Nervous System Diseases
 Brain Diseases Antibodies  >77 publications with TPO and Brain Diseases
Organs/Tissues associated with TPO elisa kit
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >2153 publications with TPO and Blood
 Thyroid Antibodies  >1745 publications with TPO and Thyroid
 Brain Antibodies  >165 publications with TPO and Brain
 Spleen Antibodies  >120 publications with TPO and Spleen
 Muscle Antibodies  >62 publications with TPO and Muscle
 Heart Antibodies  >52 publications with TPO and Heart
 Connective Tissue Antibodies  >25 publications with TPO and Connective Tissue
 Adipose Tissue Antibodies  >11 publications with TPO and Adipose Tissue
 Intestine Antibodies  >9 publications with TPO and Intestine
 Testis Antibodies  >5 publications with TPO and Testis
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