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p16 elisa kit :: Rat Antioncogene p16 protein ELISA Kit

Scan QR to view Datasheet Catalog #    MBS721562
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $455 +1 FREE 8GB USB
96-Strip-Wells  /  $660 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,925 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,490 +6 FREE 8GB USB
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Antioncogene p16 protein, ELISA Kit

★Popular Item★
 Also Known As   

Rat Antioncogene p16 protein ELISA Kit

 Product Gene Name   

p16 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    gene 606719
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 Species Reactivity    Rat
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 Specificity    This assay has high sensitivity and excellent specificity for detection of ?1-MG. No significant cross-reactivity or interference between ?1-MG and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ?1-MG and all the analogues, therefore, cross reaction may still exist in some cases.
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 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Assay Type    Sandwich
 Detection Range    0.5-10 ng/mL
 Sensitivity    0.1 ng/mL
 Intended Uses    This ?1-MG ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat ?1-MG. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of p16 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for p16 purchase    MBS721562 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Antioncogene p16 protein, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.5-10 ng/mL in biological research samples containing p16, with an estimated sensitivity of 0.1 ng/mL. The ELISA analytical biochemical technique of the MBS721562 kit is based on p16 antibody-p16 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect p16 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, p16. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for p16 elisa kit

   Principle of the assay: ?1-MG ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-?1-MG antibody and an ?1-MG-HRP conjugate. The assay sample and buffer are incubated together with ?1-MG-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ?1-MG concentration since ?1-MG from samples and ?1-MG-HRP conjugate compete for the anti-?1-MG antibody binding site. Since the number of sites is limited, as more sites are occupied by ?1-MG from the sample, fewer sites are left to bind ?1-MG-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ?1-MG concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for p16 elisa kit    Cancer
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 Typical Testing Data/Standard Curve (for reference only) of p16 elisa kit    p16 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS721562. Click to request current manual
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NCBI/Uniprot data below describe general gene information for p16. It may not necessarily be applicable to this product.
 NCBI GI #    89355819
 NCBI GeneID    1029
 NCBI Accession #    ABD72255.1 [Other Products]
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 UniProt Secondary Accession #    O95440; Q15191; Q5VVJ5; Q96B52; Q9NP05; A5X2G7; D3DRK1 [Other Products]
 UniProt Related Accession #    P42771; Q8N726 [Other Products]
 Molecular Weight    8,731 Da
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 NCBI Official Full Name    p16, partial
 NCBI Official Synonym Full Names    cyclin-dependent kinase inhibitor 2A
 NCBI Official Symbol    CDKN2A [Similar Products]
 NCBI Official Synonym Symbols   
ARF; MLM; P14; P16; P19; CMM2; INK4; MTS1; TP16; CDK4I; CDKN2; INK4A; MTS-1; P14ARF; P19ARF; P16INK4; P16INK4A; P16-INK4A
[Similar Products]
 NCBI Protein Information    cyclin-dependent kinase inhibitor 2A; CDK4 inhibitor p16-INK4; multiple tumor suppressor 1; cell cycle negative regulator beta; cyclin-dependent kinase 4 inhibitor A; cyclin-dependent kinase inhibitor 2A (melanoma, p16, inhibits CDK4)
 UniProt Protein Name    Cyclin-dependent kinase inhibitor 2A, isoforms 1/2/3
 UniProt Synonym Protein Names   
Cyclin-dependent kinase 4 inhibitor A; CDK4I; Multiple tumor suppressor 1; MTS-1; p16-INK4a; p16-INK4; p16INK4A
 UniProt Gene Name    CDKN2A [Similar Products]
 UniProt Synonym Gene Names    CDKN2; MTS1; CDK4I; MTS-1; p16-INK4; p16INK4A [Similar Products]
 UniProt Entry Name    CD2A1_HUMAN
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 NCBI Summary for p16    This gene generates several transcript variants which differ in their first exons. At least three alternatively spliced variants encoding distinct proteins have been reported, two of which encode structurally related isoforms known to function as inhibitors of CDK4 kinase. The remaining transcript includes an alternate first exon located 20 Kb upstream of the remainder of the gene; this transcript contains an alternate open reading frame (ARF) that specifies a protein which is structurally unrelated to the products of the other variants. This ARF product functions as a stabilizer of the tumor suppressor protein p53 as it can interact with, and sequester, the E3 ubiquitin-protein ligase MDM2, a protein responsible for the degradation of p53. In spite of the structural and functional differences, the CDK inhibitor isoforms and the ARF product encoded by this gene, through the regulatory roles of CDK4 and p53 in cell cycle G1 progression, share a common functionality in cell cycle G1 control. This gene is frequently mutated or deleted in a wide variety of tumors, and is known to be an important tumor suppressor gene. [provided by RefSeq, Sep 2012]
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 UniProt Comments for p16    p16-INK4A: a cell-cycle regulatory protein that interacts with CDK4 and CDK6, inhibiting their ability to interact with cyclins D. Inhibits the phosphorylation of the retinoblastoma protein by CDK4 or CDK6, and entry into the S phase of the cell cycle. The p16INK4A and p14ARF proteins are encoded by CDKN2A, a known tumour suppressor gene in multiple cancers. CDKN2A is inactivated in 72% of cases of lung squamous cell carcinoma: 21% by epigenetic silencing by methylation, 18% inactivating mutation, 4% by exon 1b skipping, and 29% by homozygous deletion. Defects in CDKN2A are the cause of familial atypical multiple mole melanoma-pancreatic carcinoma syndrome, Li-Fraumeni syndrome, and the melanoma-astrocytoma syndrome. The melanoma-astrocytoma syndrome is characterized by a dual predisposition to melanoma and neural system tumors, commonly astrocytoma. Four alternatively spliced p16 isoforms have been reported. Two alternatively spliced isoforms of ARF have been reported.

Protein type: Tumor suppressor; Nucleolus; Cell cycle regulation

Chromosomal Location of Human Ortholog: 9p21

Cellular Component: nucleoplasm; nuclear body; protein complex; mitochondrion; cytoplasm; nucleolus; nucleus; cytosol

Molecular Function: cyclin-dependent protein kinase inhibitor activity; NF-kappaB binding; protein binding; DNA binding; p53 binding; ubiquitin-protein ligase inhibitor activity; protein kinase binding; transcription factor binding

Biological Process: protein polyubiquitination; positive regulation of apoptosis; positive regulation of transcription, DNA-dependent; regulation of protein stability; negative regulation of B cell proliferation; regulation of protein export from nucleus; negative regulation of cell proliferation; apoptotic mitochondrial changes; regulation of G2/M transition of mitotic cell cycle; somatic stem cell division; cell cycle arrest; caspase activation; negative regulation of immature T cell proliferation in the thymus; protein destabilization; protein stabilization; transcription, DNA-dependent; negative regulation of cyclin-dependent protein kinase activity; negative regulation of cell-matrix adhesion; positive regulation of protein sumoylation; inhibition of NF-kappaB transcription factor; Ras protein signal transduction; negative regulation of ubiquitin-protein ligase activity; negative regulation of phosphorylation; negative regulation of protein kinase activity; positive regulation of transcription from RNA polymerase II promoter; mitotic cell cycle; positive regulation of DNA damage response, signal transduction by p53 class mediator; negative regulation of cell growth; negative regulation of transcription, DNA-dependent; rRNA processing; G1/S transition of mitotic cell cycle

Disease: Melanoma-astrocytoma Syndrome; Melanoma, Cutaneous Malignant, Susceptibility To, 2; Melanoma-pancreatic Cancer Syndrome
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 Research Articles on p16    1. p16ink4a protein expression was associated with HPV18 infection and TERC expression in esophageal squamous cell carcinoma.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with p16 elisa kitPathways associated with p16 elisa kit
 Reference Product  PubMed Publications
 CDK4 elisa kit  >1000 publications with p16 and CDK4
 CCND1 elisa kit  >872 publications with p16 and CCND1
 TP53 elisa kit  >640 publications with p16 and TP53
 MYC elisa kit  >445 publications with p16 and MYC
 MDM2 elisa kit  >368 publications with p16 and MDM2
 CDK6 elisa kit  >306 publications with p16 and CDK6
 CDK2 elisa kit  >271 publications with p16 and CDK2
 NPM1 elisa kit  >4 publications with p16 and NPM1
 UBC elisa kit  >1 publications with p16 and UBC
 Products by Pathway  Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptosis Modulation And Signaling Pathway antibodies  Apoptosis Modulation And Signaling Pathway Diagram
 Bladder Cancer Pathway antibodies  Bladder Cancer Pathway Diagram
 Bladder Cancer Pathway antibodies  Bladder Cancer Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle, Mitotic Pathway antibodies  Cell Cycle, Mitotic Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cellular Senescence Pathway antibodies  Cellular Senescence Pathway Diagram
Diseases associated with p16 elisa kitOrgans/Tissues associated with p16 elisa kit
 Disease Name  Pubmed Publications
 Adenocarcinoma Antibodies  >1256 publications with p16 and Adenocarcinoma
 Urogenital Neoplasms Antibodies  >1183 publications with p16 and Urogenital Neoplasms
 Cell Transformation, Neoplastic Antibodies  >732 publications with p16 and Cell Transformation, Neoplastic
 Lymphoma Antibodies  >686 publications with p16 and Lymphoma
 Lung Neoplasms Antibodies  >656 publications with p16 and Lung Neoplasms
 Skin Neoplasms Antibodies  >628 publications with p16 and Skin Neoplasms
 Neoplasms, Experimental Antibodies  >617 publications with p16 and Neoplasms, Experimental
 Nervous System Diseases Antibodies  >589 publications with p16 and Nervous System Diseases
 Glioma Antibodies  >486 publications with p16 and Glioma
 Pancreatic Neoplasms Antibodies  >440 publications with p16 and Pancreatic Neoplasms
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >903 publications with p16 and Blood
 Skin Antibodies  >799 publications with p16 and Skin
 Lung Antibodies  >796 publications with p16 and Lung
 Brain Antibodies  >498 publications with p16 and Brain
 Liver Antibodies  >468 publications with p16 and Liver
 Bone Antibodies  >375 publications with p16 and Bone
 Lymph Node Antibodies  >328 publications with p16 and Lymph Node
 Lymph Antibodies  >326 publications with p16 and Lymph
 Stomach Antibodies  >297 publications with p16 and Stomach
 Cervix Antibodies  >241 publications with p16 and Cervix
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