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Apob elisa kit :: Rat Apolipoprotein B ELISA Kit

Scan QR to view Datasheet Catalog #    MBS765546
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $300 +1 FREE 8GB USB
96-Strip-Wells  /  $415 +1 FREE 8GB USB
5x96-Strip-Wells  /  $1,825 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,425 +4 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Apolipoprotein B (Apob), ELISA Kit

★Popular Item★
 Also Known As   

Rat Apolipoprotein B ELISA Kit

 Product Synonym Names    Apob (Apolipoprotein B)/APOB/FLDB/apoB-48/apolipoprotein B48/FLDB/LDLCQ4
 Product Gene Name   

Apob elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS765546 COA    COA PDF
 OMIM    107730
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 Species Reactivity    Rat
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Apob . No significant cross-reactivity or interference between Apob and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates and other biological fluids
 Assay Type    Sandwich
 Detection Range    3.125-200ng/ml
 Sensitivity    <1.875ng/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Apob were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
 Inter-assay Precision    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Apob were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
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 Preparation and Storage    Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of Apob elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for Apob purchase    MBS765546 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Apolipoprotein B (Apob) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 3.125-200ng/ml in biological research samples containing Apob, with an estimated sensitivity of <1.875ng/ml. The ELISA analytical biochemical technique of the MBS765546 kit is based on Apob antibody-Apob antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Apob antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Apob. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for Apob elisa kit

   Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- Apob antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- Apob antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Apob amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Apob can be calculated.
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 Typical Testing Data/Standard Curve (for reference only) of Apob elisa kit    Apob elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS765546. Click to request current manual
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NCBI/Uniprot data below describe general gene information for Apob. It may not necessarily be applicable to this product.
 NCBI GI #    553189
 NCBI GeneID    338
 NCBI Accession #    AAA51752.1 [Other Products]
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 UniProt Secondary Accession #    O00502; P78479; P78480; P78481; Q13779; Q13785; Q13786; Q13787; Q13788; Q4ZG63; Q53QC8 [Other Products]
 UniProt Related Accession #    P04114 [Other Products]
 Molecular Weight    515,605 Da
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 NCBI Official Full Name    apolipoprotein B, partial
 NCBI Official Synonym Full Names    apolipoprotein B
 NCBI Official Symbol    APOB [Similar Products]
 NCBI Official Synonym Symbols   
FLDB; LDLCQ4; apoB-48; apoB-100
[Similar Products]
 NCBI Protein Information    apolipoprotein B-100
 UniProt Protein Name    Apolipoprotein B-100
 Protein Family    Apolipoprotein
 UniProt Gene Name    APOB [Similar Products]
 UniProt Synonym Gene Names    Apo B-100; Apo B-48 [Similar Products]
 UniProt Entry Name    APOB_HUMAN
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 NCBI Summary for Apob    This gene product is the main apolipoprotein of chylomicrons and low density lipoproteins. It occurs in plasma as two main isoforms, apoB-48 and apoB-100: the former is synthesized exclusively in the gut and the latter in the liver. The intestinal and the hepatic forms of apoB are encoded by a single gene from a single, very long mRNA. The two isoforms share a common N-terminal sequence. The shorter apoB-48 protein is produced after RNA editing of the apoB-100 transcript at residue 2180 (CAA->UAA), resulting in the creation of a stop codon, and early translation termination. Mutations in this gene or its regulatory region cause hypobetalipoproteinemia, normotriglyceridemic hypobetalipoproteinemia, and hypercholesterolemia due to ligand-defective apoB, diseases affecting plasma cholesterol and apoB levels. [provided by RefSeq, Jul 2008]
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 UniProt Comments for Apob    APOB: Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor. Defects in APOB are a cause of familial hypobetalipoproteinemia type 1 (FHBL1). A disorder characterized by highly reduced plasma concentrations of low density lipoproteins, and dietary fat malabsorption. Clinical presentation may vary from no symptoms to severe gastrointestinal and neurological dysfunction similar to abetalipoproteinemia. Defects in APOB are a cause of familial ligand-defective apolipoprotein B-100 (FDB). FDB is a dominantly inherited disorder of lipoprotein metabolism leading to hypercholesterolemia and increased proneness to coronary artery disease (CAD). The plasma cholesterol levels are dramatically elevated due to impaired clearance of LDL particles by defective APOB/E receptors. Defects in APOB associated with defects in other genes (polygenic) can contribute to hypocholesterolemia.

Protein type: Carrier; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 2p24-p23

Cellular Component: actin cytoskeleton; cell soma; chylomicron; cytoplasm; cytosol; early endosome; endoplasmic reticulum lumen; endoplasmic reticulum membrane; endosome membrane; extracellular region; extracellular space; Golgi apparatus; intracellular membrane-bound organelle; plasma membrane

Molecular Function: cholesterol transporter activity; heparin binding; low-density lipoprotein receptor binding; phospholipid binding; protein binding

Biological Process: artery morphogenesis; blood coagulation; cholesterol efflux; cholesterol homeostasis; cholesterol metabolic process; cholesterol transport; fat-soluble vitamin metabolic process; fertilization; in utero embryonic development; leukocyte migration; lipoprotein biosynthetic process; lipoprotein catabolic process; lipoprotein metabolic process; lipoprotein transport; nervous system development; phototransduction, visible light; post-embryonic development; receptor-mediated endocytosis; regulation of cholesterol biosynthetic process; response to carbohydrate stimulus; response to lipopolysaccharide; response to selenium ion; response to virus; retinoid metabolic process; sperm motility; spermatogenesis; triacylglycerol catabolic process; triacylglycerol mobilization; vitamin metabolic process

Disease: Hypercholesterolemia, Autosomal Dominant, Type B; Hypobetalipoproteinemia, Familial, 1
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 Research Articles on Apob    1. The hypertriglyceridemia of type 2 diabetes mellitus patients with diabetic nephropathy is not due to hepatic VLDL-Apo B 100 overproduction
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with Apob elisa kitPathways associated with Apob elisa kit
 Reference Product  PubMed Publications
 APOE elisa kit  >1519 publications with Apob and APOE
 APOA1 elisa kit  >542 publications with Apob and APOA1
 LDLR elisa kit  >481 publications with Apob and LDLR
 LPL elisa kit  >245 publications with Apob and LPL
 LPA elisa kit  >80 publications with Apob and LPA
 APOC3 elisa kit  >62 publications with Apob and APOC3
 MTTP elisa kit  >57 publications with Apob and MTTP
 APOA2 elisa kit  >21 publications with Apob and APOA2
 Products by Pathway  Pathway Diagram
 Binding And Uptake Of Ligands By Scavenger Receptors Pathway antibodies  Binding And Uptake Of Ligands By Scavenger Receptors Pathway Diagram
 Cell Surface Interactions At The Vascular Wall Pathway antibodies  Cell Surface Interactions At The Vascular Wall Pathway Diagram
 Chylomicron-mediated Lipid Transport Pathway antibodies  Chylomicron-mediated Lipid Transport Pathway Diagram
 FOXA1 Transcription Factor Network Pathway antibodies  FOXA1 Transcription Factor Network Pathway Diagram
 Fat Digestion And Absorption Pathway antibodies  Fat Digestion And Absorption Pathway Diagram
 Fat Digestion And Absorption Pathway antibodies  Fat Digestion And Absorption Pathway Diagram
 Hemostasis Pathway antibodies  Hemostasis Pathway Diagram
 LDL-mediated Lipid Transport Pathway antibodies  LDL-mediated Lipid Transport Pathway Diagram
 Lipid Digestion, Mobilization, And Transport Pathway antibodies  Lipid Digestion, Mobilization, And Transport Pathway Diagram
 Lipoprotein Metabolism Pathway antibodies  Lipoprotein Metabolism Pathway Diagram
Diseases associated with Apob elisa kitOrgans/Tissues associated with Apob elisa kit
 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >3636 publications with Apob and Cardiovascular Diseases
 Dyslipidemias Antibodies  >3138 publications with Apob and Dyslipidemias
 Atherosclerosis Antibodies  >2697 publications with Apob and Atherosclerosis
 Hyperlipidemias Antibodies  >2551 publications with Apob and Hyperlipidemias
 Coronary Disease Antibodies  >1932 publications with Apob and Coronary Disease
 Heart Diseases Antibodies  >1684 publications with Apob and Heart Diseases
 Hypercholesterolemia Antibodies  >1559 publications with Apob and Hypercholesterolemia
 Hyperlipoproteinemia Type II Antibodies  >753 publications with Apob and Hyperlipoproteinemia Type II
 Hypertriglyceridemia Antibodies  >648 publications with Apob and Hypertriglyceridemia
 Liver Diseases Antibodies  >608 publications with Apob and Liver Diseases
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >8410 publications with Apob and Blood
 Liver Antibodies  >2339 publications with Apob and Liver
 Heart Antibodies  >1572 publications with Apob and Heart
 Intestine Antibodies  >621 publications with Apob and Intestine
 Kidney Antibodies  >446 publications with Apob and Kidney
 Muscle Antibodies  >277 publications with Apob and Muscle
 Brain Antibodies  >163 publications with Apob and Brain
 Lung Antibodies  >146 publications with Apob and Lung
 Spleen Antibodies  >59 publications with Apob and Spleen
 Embryonic Tissue Antibodies  >58 publications with Apob and Embryonic Tissue
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