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APOE elisa kit :: Rat Apolipoprotein E ELISA Kit

Scan QR to view Datasheet Catalog #    MBS721752
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells-(Competitive)  /  $455 +1 FREE 8GB USB
48-Strip-Wells-(Sandwich)  /  $455 +1 FREE 8GB USB
96-Strip-Wells-(Competitive)  /  $660 +1 FREE 8GB USB
96-Strip-Wells-(Sandwich)  /  $660 +1 FREE 8GB USB
5x96-Strip-Wells-(Competitive)  /  $2,925 +3 FREE 8GB USB
5x96-Strip-Wells-(Sandwich)  /  $2,925 +3 FREE 8GB USB
10x96-Strip-Wells-(Competitive)  /  $5,490 +6 FREE 8GB USB
10x96-Strip-Wells-(Sandwich)  /  $5,490 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Apolipoprotein E (APOE), ELISA Kit

★Popular Item★
 Also Known As   

Rat Apolipoprotein E ELISA Kit

 Product Gene Name   

APOE elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    611771
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 Species Reactivity    Rat
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 Specificity    This assay has high sensitivity and excellent specificity for detection of ApoE. No significant cross-reactivity or interference between ApoE and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ApoE and all the analogues, therefore, cross reaction may still exist in some cases.
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 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Assay Type    Competitive or Sandwich
 Detection Range    50-1000ng/mL
 Sensitivity    1.0ng/ml
 Intended Uses    This ApoE ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat ApoE. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of APOE elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for APOE purchase    MBS721752 is a ready-to-use microwell, strip plate Competitive or Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Apolipoprotein E (APOE) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 50-1000ng/mL in biological research samples containing APOE, with an estimated sensitivity of 1.0ng/ml. The ELISA analytical biochemical technique of the MBS721752 kit is based on APOE antibody-APOE antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect APOE antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, APOE. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for APOE elisa kit

   Principle of the assay: ApoE ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ApoE antibody and an ApoE-HRP conjugate. The assay sample and buffer are incubated together with ApoE-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ApoE concentration since ApoE from samples and ApoE-HRP conjugate compete for the anti-ApoE antibody binding site. Since the number of sites is limited, as more sites are occupied by ApoE from the sample, fewer sites are left to bind ApoE-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ApoE concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for APOE elisa kit    Neurobiology
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 Typical Testing Data/Standard Curve (for reference only) of APOE elisa kit    APOE elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS721752. Click to request current manual
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NCBI/Uniprot data below describe general gene information for APOE. It may not necessarily be applicable to this product.
 NCBI GI #    4557325
 NCBI GeneID    348
 NCBI Accession #    NP_000032.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_000041.2 [Other Products]
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 UniProt Secondary Accession #    Q9P2S4; B2RC15; C0JYY5 [Other Products]
 UniProt Related Accession #    P02649 [Other Products]
 Molecular Weight    36,154 Da
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 NCBI Official Full Name    apolipoprotein E
 NCBI Official Synonym Full Names    apolipoprotein E
 NCBI Official Symbol    APOE [Similar Products]
 NCBI Official Synonym Symbols   
AD2; LPG; LDLCQ5
[Similar Products]
 NCBI Protein Information    apolipoprotein E; apo-E; apolipoprotein E3
 UniProt Protein Name    Apolipoprotein E
 Protein Family    Apolipoprotein
 UniProt Gene Name    APOE [Similar Products]
 UniProt Synonym Gene Names    Apo-E [Similar Products]
 UniProt Entry Name    APOE_HUMAN
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 NCBI Summary for APOE    Chylomicron remnants and very low density lipoprotein (VLDL) remnants are rapidly removed from the circulation by receptor-mediated endocytosis in the liver. Apolipoprotein E, a main apoprotein of the chylomicron, binds to a specific receptor on liver cells and peripheral cells. ApoE is essential for the normal catabolism of triglyceride-rich lipoprotein constituents. The APOE gene is mapped to chromosome 19 in a cluster with APOC1 and APOC2. Defects in apolipoprotein E result in familial dysbetalipoproteinemia, or type III hyperlipoproteinemia (HLP III), in which increased plasma cholesterol and triglycerides are the consequence of impaired clearance of chylomicron and VLDL remnants. [provided by RefSeq, Jul 2008]
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 UniProt Comments for APOE    APOE: Mediates the binding, internalization, and catabolism of lipoprotein particles. It can serve as a ligand for the LDL (apo B/E) receptor and for the specific apo-E receptor (chylomicron remnant) of hepatic tissues. Defects in APOE are a cause of hyperlipoproteinemia type 3 (HLPP3); also known as familial dysbetalipoproteinemia. Individuals with HLPP3 are clinically characterized by xanthomas, yellowish lipid deposits in the palmar crease, or less specific on tendons and on elbows. The disorder rarely manifests before the third decade in men. In women, it is usually expressed only after the menopause. The vast majority of the patients are homozygous for APOE*2 alleles. More severe cases of HLPP3 have also been observed in individuals heterozygous for rare APOE variants. The influence of APOE on lipid levels is often suggested to have major implications for the risk of coronary artery disease (CAD). Individuals carrying the common APOE*4 variant are at higher risk of CAD. Genetic variations in APOE are associated with Alzheimer disease type 2 (AD2). It is a late-onset neurodegenerative disorder characterized by progressive dementia, loss of cognitive abilities, and deposition of fibrillar amyloid proteins as intraneuronal neurofibrillary tangles, extracellular amyloid plaques and vascular amyloid deposits. The major constituent of these plaques is the neurotoxic amyloid-beta-APP 40-42 peptide (s), derived proteolytically from the transmembrane precursor protein APP by sequential secretase processing. The cytotoxic C-terminal fragments (CTFs) and the caspase-cleaved products such as C31 derived from APP, are also implicated in neuronal death. The APOE*4 allele is genetically associated with the common late onset familial and sporadic forms of Alzheimer disease. Risk for AD increased from 20% to 90% and mean age at onset decreased from 84 to 68 years with increasing number of APOE*4 alleles in 42 families with late onset AD. Thus APOE*4 gene dose is a major risk factor for late onset AD and, in these families, homozygosity for APOE*4 was virtually sufficient to cause AD by age 80. The mechanism by which APOE*4 participates in pathogenesis is not known. Defects in APOE are a cause of sea-blue histiocyte disease (SBHD); also known as sea-blue histiocytosis. This disorder is characterized by splenomegaly, mild thrombocytopenia and, in the bone marrow, numerous histiocytes containing cytoplasmic granules which stain bright blue with the usual hematologic stains. The syndrome is the consequence of an inherited metabolic defect analogous to Gaucher disease and other sphingolipidoses. Defects in APOE are a cause of lipoprotein glomerulopathy (LPG). LPG is an uncommon kidney disease characterized by proteinuria, progressive kidney failure, and distinctive lipoprotein thrombi in glomerular capillaries. It mainly affects people of Japanese and Chinese origin. The disorder has rarely been described in Caucasians. Belongs to the apolipoprotein A1/A4/E family.

Protein type: Secreted, signal peptide; Secreted; Lipid-binding

Chromosomal Location of Human Ortholog: 19q13.2

Cellular Component: Golgi apparatus; extracellular space; microtubule; endoplasmic reticulum; lysosome; dendrite; early endosome; extracellular region; nuclear envelope; extracellular matrix; chylomicron; extrinsic to external side of plasma membrane; membrane; cell soma; late endosome; cytoplasm; plasma membrane; nucleus; vesicle

Molecular Function: heparin binding; lipid transporter activity; identical protein binding; protein homodimerization activity; metal chelating activity; beta-amyloid binding; cholesterol binding; antioxidant activity; protein binding; low-density lipoprotein receptor binding; hydroxyapatite binding; cholesterol transporter activity; phospholipid binding; tau protein binding; lipid binding

Biological Process: phototransduction, visible light; lipoprotein catabolic process; negative regulation of MAP kinase activity; cGMP-mediated signaling; positive regulation of axon extension; positive regulation of membrane protein ectodomain proteolysis; axon regeneration in the peripheral nervous system; synaptic transmission, cholinergic; intracellular transport; triacylglycerol catabolic process; oligodendrocyte differentiation; negative regulation of neuron apoptosis; cholesterol catabolic process; long-chain fatty acid transport; cholesterol metabolic process; regulation of Cdc42 protein signal transduction; positive regulation of nitric-oxide synthase activity; negative regulation of blood coagulation; lipoprotein metabolic process; regulation of axon extension; positive regulation of lipid biosynthetic process; negative regulation of blood vessel endothelial cell migration; maintenance of cellular localization; response to reactive oxygen species; cholesterol homeostasis; response to ethanol; positive regulation of cGMP biosynthetic process; regulation of gene expression; lipoprotein biosynthetic process; protein import; negative regulation of endothelial cell proliferation; nitric oxide mediated signal transduction; regulation of neuronal synaptic plasticity; response to dietary excess; vasodilation; response to insulin stimulus; positive regulation of low-density lipoprotein receptor catabolic process; phospholipid efflux; negative regulation of cholesterol biosynthetic process; retinoid metabolic process; aging; receptor-mediated endocytosis; response to retinoic acid; negative regulation of lipid biosynthetic process; neurite regeneration; cholesterol efflux; cytoskeleton organization and biogenesis; cellular calcium ion homeostasis; G-protein coupled receptor protein signaling pathway; triacylglycerol metabolic process; reverse cholesterol transport; negative regulation of inflammatory response; fatty acid homeostasis; artery morphogenesis

Disease: Macular Degeneration, Age-related, 1; Alzheimer Disease 2; Alzheimer Disease 4; Lipoprotein Glomerulopathy; Sea-blue Histiocyte Disease
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 Research Articles on APOE    1. APOE polymorphism is associated with carotid atherosclerosis in a South Korean population, and this association was partly mediated through blood lipids.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with APOE elisa kitPathways associated with APOE elisa kit
 Reference Product  PubMed Publications
 APOB elisa kit  >1454 publications with APOE and APOB
 LDLR elisa kit  >530 publications with APOE and LDLR
 LPL elisa kit  >320 publications with APOE and LPL
 SCARB1 elisa kit  >111 publications with APOE and SCARB1
 MAPT elisa kit  >95 publications with APOE and MAPT
 LRP1 elisa kit  >72 publications with APOE and LRP1
 APOC3 elisa kit  >69 publications with APOE and APOC3
 VLDLR elisa kit  >50 publications with APOE and VLDLR
 LIPC elisa kit  >41 publications with APOE and LIPC
 LRP8 elisa kit  >14 publications with APOE and LRP8
 Products by Pathway  Pathway Diagram
 Alzheimer's Disease Pathway antibodies  Alzheimer's Disease Pathway Diagram
 Alzheimer's Disease Pathway antibodies  Alzheimer's Disease Pathway Diagram
 Alzheimers Disease Pathway antibodies  Alzheimers Disease Pathway Diagram
 Binding And Uptake Of Ligands By Scavenger Receptors Pathway antibodies  Binding And Uptake Of Ligands By Scavenger Receptors Pathway Diagram
 Chylomicron-mediated Lipid Transport Pathway antibodies  Chylomicron-mediated Lipid Transport Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Diseases Associated With Visual Transduction Pathway antibodies  Diseases Associated With Visual Transduction Pathway Diagram
 HDL-mediated Lipid Transport Pathway antibodies  HDL-mediated Lipid Transport Pathway Diagram
 Lipid Digestion, Mobilization, And Transport Pathway antibodies  Lipid Digestion, Mobilization, And Transport Pathway Diagram
 Lipoprotein Metabolism Pathway antibodies  Lipoprotein Metabolism Pathway Diagram
Diseases associated with APOE elisa kitOrgans/Tissues associated with APOE elisa kit
 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >5848 publications with APOE and Cardiovascular Diseases
 Alzheimer Disease Antibodies  >5457 publications with APOE and Alzheimer Disease
 Atherosclerosis Antibodies  >4620 publications with APOE and Atherosclerosis
 Arteriosclerosis Antibodies  >3858 publications with APOE and Arteriosclerosis
 Hyperlipidemias Antibodies  >1784 publications with APOE and Hyperlipidemias
 Inflammation Antibodies  >1499 publications with APOE and Inflammation
 Cognition Disorders Antibodies  >1276 publications with APOE and Cognition Disorders
 Plaque, Atherosclerotic Antibodies  >1147 publications with APOE and Plaque, Atherosclerotic
 Coronary Disease Antibodies  >1102 publications with APOE and Coronary Disease
 Hypercholesterolemia Antibodies  >1025 publications with APOE and Hypercholesterolemia
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >4170 publications with APOE and Brain
 Vascular Antibodies  >3911 publications with APOE and Vascular
 Liver Antibodies  >1809 publications with APOE and Liver
 Heart Antibodies  >1513 publications with APOE and Heart
 Muscle Antibodies  >1136 publications with APOE and Muscle
 Nerve Antibodies  >615 publications with APOE and Nerve
 Bone Antibodies  >558 publications with APOE and Bone
 Kidney Antibodies  >483 publications with APOE and Kidney
 Lung Antibodies  >262 publications with APOE and Lung
 Spleen Antibodies  >224 publications with APOE and Spleen
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