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AQP-2 elisa kit :: Rat Aquaporin 2 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS765367
Unit / Price
48-Strip-Wells  /  $295 +1 FREE 8GB USB
96-Strip-Wells  /  $390 +1 FREE 8GB USB
5x96-Strip-Wells  /  $1,615 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,015 +3 FREE 8GB USB
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Aquaporin 2 (AQP-2), ELISA Kit

 Also Known As   

Rat Aquaporin 2 ELISA Kit

 Product Synonym Names    AQP-2/Aquaporin-2/Water channel protein for renal collecting duct/WCH-CD/Collecting duct water channel protein/ADH water channel/Aquaporin-CD (AQP-CD)
 Product Gene Name   

AQP-2 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Manual Insert    Download PDF Manual View PDF Manual
 OMIM    107777
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 Species Reactivity    Rat
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Rat AQP-2. No significant cross-reactivity or interference between Rat AQP-2 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the crossreactivity detection between Rat AQP-2 and all the analogues, therefore, cross reaction may still exist.
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 Samples    Serum, plasma and other biological fluids.
 Assay Type    Competitive ELISA, Coated with Antigen
 Detection Range    1.563-100ng/ml
 Sensitivity    0.938ng/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Rat AQP-2 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
 Inter-assay Precision    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Rat AQP-2 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<10%
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 Preparation and Storage    Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay.
 Other Notes    Small volumes of AQP-2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for AQP-2 purchase    MBS765367 is a ready-to-use microwell, strip plate Competitive, Coated with Antigen ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Aquaporin 2 (AQP-2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 1.563-100ng/ml in biological research samples containing AQP-2, with an estimated sensitivity of 0.938ng/ml. The ELISA analytical biochemical technique of the MBS765367 kit is based on AQP-2 antibody-AQP-2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect AQP-2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, AQP-2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for AQP-2 elisa kit

   Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Rat AQP-2 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Rat AQP-2 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Rat AQP-2 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Rat AQP-2 can be calculated.
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 Typical Testing Data of AQP-2 elisa kit    AQP-2 elisa kit Typical Testing Data image
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 Typical Standard Curve of AQP-2 elisa kit    AQP-2 elisa kit Typical Standard Curve image
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Sample Manual Insert of MBS765367
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NCBI/Uniprot data below describe general gene information for AQP-2. It may not necessarily be applicable to this product.
 NCBI GI #    5052748
 NCBI GeneID    359
 NCBI Accession #    AAD38692.1 [Other Products]
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 UniProt Secondary Accession #    Q9UD68 [Other Products]
 UniProt Related Accession #    P41181 [Other Products]
 Molecular Weight    28,837 Da
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 NCBI Official Full Name    aquaporin 2
 NCBI Official Synonym Full Names    aquaporin 2
 NCBI Official Symbol    AQP2 [Similar Products]
 NCBI Official Synonym Symbols   
[Similar Products]
 NCBI Protein Information    aquaporin-2
 UniProt Protein Name    Aquaporin-2
 UniProt Synonym Protein Names   
ADH water channel; Aquaporin-CD; AQP-CD; Collecting duct water channel protein; WCH-CD; Water channel protein for renal collecting duct
 UniProt Gene Name    AQP2 [Similar Products]
 UniProt Synonym Gene Names    AQP-2; AQP-CD [Similar Products]
 UniProt Entry Name    AQP2_HUMAN
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 NCBI Summary for AQP-2    This gene encodes a water channel protein located in the kidney collecting tubule. It belongs to the MIP/aquaporin family, some members of which are clustered together on chromosome 12q13. Mutations in this gene have been linked to autosomal dominant and recessive forms of nephrogenic diabetes insipidus. [provided by RefSeq, Oct 2008]
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 UniProt Comments for AQP-2    AQP2: Forms a water-specific channel that provides the plasma membranes of renal collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient. Expressed in renal collecting tubules. Belongs to the MIP/aquaporin (TC 1.A.8) family.

Protein type: Transporter; Membrane protein, multi-pass; Membrane protein, integral; Channel, misc.; Transporter, aquaporin family

Chromosomal Location of Human Ortholog: 12q12-q13

Cellular Component: apical plasma membrane; basolateral plasma membrane; Golgi apparatus; integral to membrane; membrane; plasma membrane; recycling endosome

Molecular Function: glycerol channel activity; glycerol transmembrane transporter activity; water channel activity; water transporter activity

Biological Process: cellular response to water deprivation; excretion; glycerol transport; renal water homeostasis; renal water transport; transmembrane transport; water transport

Disease: Diabetes Insipidus, Nephrogenic, Autosomal
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 Research Articles on AQP-2    1. Taken together these results provide a possible molecular mechanism explaining the increased AQP2 membrane expression under RGZ treatment: in renal cells RGZ elicits Ca(2+) transients facilitating AQP2 exposure at the apical plasma membrane
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with AQP-2 elisa kitPathways associated with AQP-2 elisa kit
 Reference Product  PubMed Publications
 AVP elisa kit  >239 publications with AQP-2 and AVP
 AQP3 elisa kit  >231 publications with AQP-2 and AQP3
 SLC12A1 elisa kit  >50 publications with AQP-2 and SLC12A1
 PRKACA elisa kit  >1 publications with AQP-2 and PRKACA
 MYO5B elisa kit  >1 publications with AQP-2 and MYO5B
 Products by Pathway  Pathway Diagram
 Aquaporin-mediated Transport Pathway antibodies  Aquaporin-mediated Transport Pathway Diagram
 Passive Transport By Aquaporins Pathway antibodies  Passive Transport By Aquaporins Pathway Diagram
 Transmembrane Transport Of Small Molecules Pathway antibodies  Transmembrane Transport Of Small Molecules Pathway Diagram
 Vasopressin Regulates Renal Water Homeostasis Via Aquaporins Pathway antibodies  Vasopressin Regulates Renal Water Homeostasis Via Aquaporins Pathway Diagram
 Vasopressin-regulated Water Reabsorption Pathway antibodies  Vasopressin-regulated Water Reabsorption Pathway Diagram
 Vasopressin-regulated Water Reabsorption Pathway antibodies  Vasopressin-regulated Water Reabsorption Pathway Diagram
Diseases associated with AQP-2 elisa kitOrgans/Tissues associated with AQP-2 elisa kit
 Disease Name  Pubmed Publications
 Kidney Diseases Antibodies  >357 publications with AQP-2 and Kidney Diseases
 Diabetes Insipidus, Nephrogenic Antibodies  >269 publications with AQP-2 and Diabetes Insipidus, Nephrogenic
 Polyuria Antibodies  >149 publications with AQP-2 and Polyuria
 Disease Models, Animal Antibodies  >101 publications with AQP-2 and Disease Models, Animal
 Cardiovascular Diseases Antibodies  >83 publications with AQP-2 and Cardiovascular Diseases
 Heart Failure Antibodies  >66 publications with AQP-2 and Heart Failure
 Hypertension Antibodies  >64 publications with AQP-2 and Hypertension
 Heart Diseases Antibodies  >57 publications with AQP-2 and Heart Diseases
 Brain Diseases Antibodies  >38 publications with AQP-2 and Brain Diseases
 Edema Antibodies  >38 publications with AQP-2 and Edema
 Organ/Tissue Name  Pubmed Publications
 Kidney Antibodies  >953 publications with AQP-2 and Kidney
 Brain Antibodies  >78 publications with AQP-2 and Brain
 Eye Antibodies  >26 publications with AQP-2 and Eye
 Testis Antibodies  >9 publications with AQP-2 and Testis
 Prostate Antibodies  >4 publications with AQP-2 and Prostate
 Cervix Antibodies  >1 publications with AQP-2 and Cervix
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