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ARO elisa kit :: Rat Aromatase (ARO) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2023052 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells  /  $285 +1 FREE 8GB USB
48-Strip-Wells  /  $530 +1 FREE 8GB USB
96-Strip-Wells  /  $715 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,910 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,280 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Aromatase (ARO), ELISA Kit

★Popular Item★
 Also Known As   

Aromatase (ARO) ELISA Kit

 Product Synonym Names    CYP19A1; CYPXIX; ARO; ARO1; CPV1; CYAR; CYP19; P450AROM; P450-AROM; Estrogen synthase; Cytochrome P450,Family 19,Subfamily A,Polypeptide 1
 Product Gene Name   

ARO elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    107910
 3D Structure    ModBase 3D Structure for P11511
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 Species Reactivity    Rat
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Aromatase (ARO).
No significant cross-reactivity or interference between Aromatase (ARO) and analogues was observed.
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 Assay Type    Double-antibody Sandwich
 Samples    Serum, Plasma, Tissue homogenates and Other Biological Fluids
 Detection Range    1.56-100ng/mL
 Sensitivity    < 0.62ng/mL
 Application    Enzyme-linked immunosorbent assay for Antigen Detection.
 Intra-assay Precision (Precision within an assay)    3 samples with low, middle and high level Aromatase (ARO) were tested 20 times on one plate, respectively.
 Inter-assay Precision (Precision between assays)    3 samples with low, middle and high level Aromatase (ARO) were tested on 3 different plates, 8 replicates in each plate.
 CV(%) =    SD/meanX100
 Intra-Assay    CV<10%
 Inter-Assay    CV<12%
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 Preparation and Storage    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
 ISO Certification    Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
 Supply Chain Verification    Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available. Please inquire.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of ARO elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for ARO purchase    MBS2023052 is a ready-to-use microwell, strip plate Double-antibody Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Aromatase (ARO) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 1.56-100ng/mL in biological research samples containing ARO, with an estimated sensitivity of < 0.62ng/mL. The ELISA analytical biochemical technique of the MBS2023052 kit is based on ARO antibody-ARO antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ARO antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ARO. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma, Tissue homogenates and Other Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for ARO elisa kit

   The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Aromatase (ARO). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Aromatase (ARO). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Aromatase (ARO), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Aromatase (ARO) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
 Product Categories/Family for ARO elisa kit    Enzyme & Kinase; Tumor immunity; Endocrinology; Hormone metabolism
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 Typical Testing Data/Standard Curve (for reference only) of ARO elisa kit    ARO elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2023052. Click to request current manual
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NCBI/Uniprot data below describe general gene information for ARO. It may not necessarily be applicable to this product.
 NCBI GI #    13904860
 NCBI GeneID    1588
 NCBI Accession #    NP_112503.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_031226.2 [Other Products]
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 UniProt Primary Accession #    P11511 [Other Products]
 UniProt Secondary Accession #    Q16731; Q3B764; Q58FA0; Q8IYJ7 [Other Products]
 UniProt Related Accession #    P11511 [Other Products]
 Molecular Weight    24,518 Da
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 NCBI Official Full Name    aromatase
 NCBI Official Synonym Full Names    cytochrome P450, family 19, subfamily A, polypeptide 1
 NCBI Official Symbol    CYP19A1 [Similar Products]
 NCBI Official Synonym Symbols   
ARO; ARO1; CPV1; CYAR; CYP19; CYPXIX; P-450AROM
[Similar Products]
 NCBI Protein Information    aromatase
 UniProt Protein Name    Aromatase
 UniProt Synonym Protein Names   
CYPXIX; Cytochrome P-450AROM; Cytochrome P450 19A1; Estrogen synthase
 UniProt Gene Name    CYP19A1 [Similar Products]
 UniProt Synonym Gene Names    ARO1; CYAR; CYP19 [Similar Products]
 UniProt Entry Name    CP19A_HUMAN
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 NCBI Summary for ARO    This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and catalyzes the last steps of estrogen biosynthesis. Mutations in this gene can result in either increased or decreased aromatase activity; the associated phenotypes suggest that estrogen functions both as a sex steroid hormone and in growth or differentiation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]
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 UniProt Comments for ARO    CYP19A1: Catalyzes the formation of aromatic C18 estrogens from C19 androgens. Belongs to the cytochrome P450 family.

Protein type: Oxidoreductase; Lipid Metabolism - androgen and estrogen; EC 1.14.14.14

Chromosomal Location of Human Ortholog: 15q21.1

Cellular Component: endoplasmic reticulum membrane; membrane; endoplasmic reticulum

Molecular Function: electron carrier activity; iron ion binding; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen; heme binding; oxygen binding

Biological Process: steroid metabolic process; xenobiotic metabolic process; androgen metabolic process; sterol metabolic process; estrogen biosynthetic process; steroid biosynthetic process

Disease: Aromatase Deficiency; Aromatase Excess Syndrome
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 Research Articles on ARO    1. The protective effect of female gender on abdominal aortic aneurysms is due to estrogen synthesis and requires the presence of both ovarian and extragonadal/peripheral aromatase.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with ARO elisa kitPathways associated with ARO elisa kit
 Reference Product  PubMed Publications
 HSD17B2 elisa kit  >317 publications with ARO and HSD17B2
 SRD5A2 elisa kit  >155 publications with ARO and SRD5A2
 CYP11B1 elisa kit  >66 publications with ARO and CYP11B1
 HSD17B1 elisa kit  >51 publications with ARO and HSD17B1
 HSD3B1 elisa kit  >44 publications with ARO and HSD3B1
 NR0B1 elisa kit  >20 publications with ARO and NR0B1
 HSD3B2 elisa kit  >16 publications with ARO and HSD3B2
 SRD5A1 elisa kit  >13 publications with ARO and SRD5A1
 HSD17B3 elisa kit  >13 publications with ARO and HSD17B3
 HSD17B6 elisa kit  >3 publications with ARO and HSD17B6
 Products by Pathway  Pathway Diagram
 Biological Oxidations Pathway antibodies  Biological Oxidations Pathway Diagram
 C19/C18-Steroid Hormone Biosynthesis, Pregnenolone => Androstenedione => Estrone Pathway antibodies  C19/C18-Steroid Hormone Biosynthesis, Pregnenolone => Androstenedione => Estrone Pathway Diagram
 C19/C18-Steroid Hormone Biosynthesis, Pregnenolone => Androstenedione => Estrone Pathway antibodies  C19/C18-Steroid Hormone Biosynthesis, Pregnenolone => Androstenedione => Estrone Pathway Diagram
 Cytochrome P450 - Arranged By Substrate Type Pathway antibodies  Cytochrome P450 - Arranged By Substrate Type Pathway Diagram
 Defective CYP11A1 Causes Adrenal Insufficiency, Congenital, With 46,XY Sex Reversal (AICSR) Pathway antibodies  Defective CYP11A1 Causes Adrenal Insufficiency, Congenital, With 46,XY Sex Reversal (AICSR) Pathway Diagram
 Defective CYP11B1 Causes Adrenal Hyperplasia 4 (AH4) Pathway antibodies  Defective CYP11B1 Causes Adrenal Hyperplasia 4 (AH4) Pathway Diagram
 Defective CYP11B2 Causes Corticosterone Methyloxidase 1 Deficiency (CMO-1 Deficiency) Pathway antibodies  Defective CYP11B2 Causes Corticosterone Methyloxidase 1 Deficiency (CMO-1 Deficiency) Pathway Diagram
 Defective CYP17A1 Causes Adrenal Hyperplasia 5 (AH5) Pathway antibodies  Defective CYP17A1 Causes Adrenal Hyperplasia 5 (AH5) Pathway Diagram
 Defective CYP19A1 Causes Aromatase Excess Syndrome (AEXS) Pathway antibodies  Defective CYP19A1 Causes Aromatase Excess Syndrome (AEXS) Pathway Diagram
 Defective CYP1B1 Causes Glaucoma Pathway antibodies  Defective CYP1B1 Causes Glaucoma Pathway Diagram
Diseases associated with ARO elisa kitOrgans/Tissues associated with ARO elisa kit
 Disease Name  Pubmed Publications
 Breast Neoplasms Antibodies  >4189 publications with ARO and Breast Neoplasms
 Ovarian Diseases Antibodies  >470 publications with ARO and Ovarian Diseases
 Osteoporosis Antibodies  >436 publications with ARO and Osteoporosis
 Adenocarcinoma Antibodies  >397 publications with ARO and Adenocarcinoma
 Neoplasms, Experimental Antibodies  >383 publications with ARO and Neoplasms, Experimental
 Infertility, Female Antibodies  >363 publications with ARO and Infertility, Female
 Endometriosis Antibodies  >321 publications with ARO and Endometriosis
 Nervous System Diseases Antibodies  >288 publications with ARO and Nervous System Diseases
 Hyperplasia Antibodies  >285 publications with ARO and Hyperplasia
 Polycystic Ovary Syndrome Antibodies  >229 publications with ARO and Polycystic Ovary Syndrome
 Organ/Tissue Name  Pubmed Publications
 Ovary Antibodies  >2386 publications with ARO and Ovary
 Blood Antibodies  >2101 publications with ARO and Blood
 Brain Antibodies  >1688 publications with ARO and Brain
 Testis Antibodies  >996 publications with ARO and Testis
 Placenta Antibodies  >813 publications with ARO and Placenta
 Liver Antibodies  >501 publications with ARO and Liver
 Embryonic Tissue Antibodies  >318 publications with ARO and Embryonic Tissue
 Vascular Antibodies  >240 publications with ARO and Vascular
 Adrenal Gland Antibodies  >189 publications with ARO and Adrenal Gland
 Lung Antibodies  >162 publications with ARO and Lung
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