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ANP elisa kit :: Rat Atrial Natriuretic Peptide ELISA Kit

Scan QR to view Datasheet Catalog #    MBS765980
Typical Standard Curve/Testing Data
Unit / Price
48-Strip-Wells  /  $300 +1 FREE 8GB USB
96-Strip-Wells  /  $415 +1 FREE 8GB USB
5x96-Strip-Wells  /  $1,825 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,425 +4 FREE 8GB USB
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 Product Name   

Atrial Natriuretic Peptide (ANP), ELISA Kit

★Popular Item★
 Also Known As   

Rat Atrial Natriuretic Peptide ELISA Kit

 Product Synonym Names    ANP/NPPA/ANF/Cardionatrin/NPPA/PND/ANF/ANPatriopeptin/ATFB6/atrial natriuretic factor/Atrial natriuretic peptide/CDD-ANF/natriuretic peptide A/natriuretic peptide precursor A/PNDcardionatrin/prepronatriodilatin/Prepronatriodilatin
 Product Gene Name   

ANP elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS765980 COA    COA PDF
 OMIM    108780
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 Species Reactivity    Rat
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 Specificity    This assay has high sensitivity and excellent specificity for detection of ANP. No significant cross-reactivity or interference between ANP and analogues is observed.
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 Samples    Serum, plasma, tissue homogenates and other biological fluids
 Assay Type    Quantitative Competitive
 Detection Range    15.625-1000pg/ml
 Sensitivity    <9.375pg/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ANP were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
 Inter-assay Precision    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ANP were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
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 Preparation and Storage    Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of ANP elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for ANP purchase    MBS765980 is a ready-to-use microwell, strip plate Quantitative Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Atrial Natriuretic Peptide (ANP) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 15.625-1000pg/ml in biological research samples containing ANP, with an estimated sensitivity of <9.375pg/ml. The ELISA analytical biochemical technique of the MBS765980 kit is based on ANP antibody-ANP antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ANP antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ANP. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for ANP elisa kit

   Principle of the Assay: This kit was based on Competitive-ELISA detection method. The microtiter plate provided in this kit has been pre-coated with ANP. During the reaction, ANP in the sample or standard competes with a fixed amount of ANP on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to ANP. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin (SABC) is added to each microplate well and incubated. Then TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of ANP in the samples is then determined by comparing the O D of the samples to the standard curve.
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 Typical Standard Curve/Testing Data of ANP elisa kit    ANP elisa kit Typical Standard Curve/Testing Data image
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Sample Manual Insert of MBS765980. Click to request current manual
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NCBI/Uniprot data below describe general gene information for ANP. It may not necessarily be applicable to this product.
 NCBI GI #    178638
 NCBI GeneID    4878
 NCBI Accession #    AAA35529.1 [Other Products]
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 UniProt Secondary Accession #    Q13766; Q5JZE1 [Other Products]
 UniProt Related Accession #    P01160 [Other Products]
 Molecular Weight    16,708 Da
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 NCBI Official Full Name    atrial natriuretic peptide
 NCBI Official Synonym Full Names    natriuretic peptide A
 NCBI Official Symbol    NPPA [Similar Products]
 NCBI Official Synonym Symbols   
[Similar Products]
 NCBI Protein Information    natriuretic peptides A
 UniProt Protein Name    Natriuretic peptides A
 UniProt Synonym Protein Names   
CDD-ANF; Cardiodilatin; CDD
 Protein Family    Andropin
 UniProt Gene Name    NPPA [Similar Products]
 UniProt Synonym Gene Names    ANP; PND; CDD; CDP; ANF; ANP [Similar Products]
 UniProt Entry Name    ANF_HUMAN
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 NCBI Summary for ANP    The protein encoded by this gene belongs to the natriuretic peptide family. Natriuretic peptides are implicated in the control of extracellular fluid volume and electrolyte homeostasis. This protein is synthesized as a large precursor (containing a signal peptide), which is processed to release a peptide from the N-terminus with similarity to vasoactive peptide, cardiodilatin, and another peptide from the C-terminus with natriuretic-diuretic activity. Mutations in this gene have been associated with atrial fibrillation familial type 6. This gene is located adjacent to another member of the natriuretic family of peptides on chromosome 1. [provided by RefSeq, Oct 2015]
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 UniProt Comments for ANP    NPPA: Hormone playing a key role in cardiovascular homeostasis through regulation of natriuresis, diuresis, and vasodilation. Also plays a role in female pregnancy by promoting trophoblast invasion and spiral artery remodeling in uterus. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3. Defects in NPPA are the cause of familial atrial fibrillation type 6 (ATFB6). Atrial fibrillation is a common disorder of cardiac rhythm that is hereditary in a small subgroup of patients. It is characterized by disorganized atrial electrical activity, progressive deterioration of atrial electromechanical function and ineffective pumping of blood into the ventricles. It can be associated with palpitations, syncope, thromboembolic stroke, and congestive heart failure. Belongs to the natriuretic peptide family.

Protein type: Secreted; Hormone; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 1p36.21

Cellular Component: extracellular region; extracellular space; mast cell granule; nucleus; perinuclear region of cytoplasm

Molecular Function: neuropeptide hormone activity; peptide hormone receptor binding; protein binding; receptor binding

Biological Process: cardiac muscle hypertrophy; cellular protein metabolic process; cGMP biosynthetic process; female pregnancy; gene expression; negative regulation of cell growth; negative regulation of systemic arterial blood pressure; neuropeptide signaling pathway; positive regulation of heart rate; receptor guanylyl cyclase signaling pathway; regulation of blood pressure; regulation of blood vessel size; response to hypoxia; response to insulin stimulus; transcription initiation from RNA polymerase II promoter

Disease: Atrial Fibrillation, Familial, 6; Atrial Standstill 2
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 Research Articles on ANP    1. Plasma MR-proANP independently predicts all-cause mortality and MACE in patients with STEMI.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with ANP elisa kitPathways associated with ANP elisa kit
 Reference Product  PubMed Publications
 NPPB elisa kit  >49 publications with ANP and NPPB
 TBX5 elisa kit  >17 publications with ANP and TBX5
 GATA4 elisa kit  >9 publications with ANP and GATA4
 NPR1 elisa kit  >9 publications with ANP and NPR1
 NPR2 elisa kit  >6 publications with ANP and NPR2
 CORIN elisa kit  >5 publications with ANP and CORIN
 NPR3 elisa kit  >5 publications with ANP and NPR3
 MEF2C elisa kit  >2 publications with ANP and MEF2C
 FOS elisa kit  >2 publications with ANP and FOS
 Products by Pathway  Pathway Diagram
 Amyloid Fiber Formation Pathway antibodies  Amyloid Fiber Formation Pathway Diagram
 Cardiac Conduction Pathway antibodies  Cardiac Conduction Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 Generic Transcription Pathway antibodies  Generic Transcription Pathway Diagram
 HIF-1 Signaling Pathway antibodies  HIF-1 Signaling Pathway Diagram
 Metabolism Of Proteins Pathway antibodies  Metabolism Of Proteins Pathway Diagram
 MicroRNAs In Cardiomyocyte Hypertrophy Pathway antibodies  MicroRNAs In Cardiomyocyte Hypertrophy Pathway Diagram
 Muscle Contraction Pathway antibodies  Muscle Contraction Pathway Diagram
 Physiological Factors Pathway antibodies  Physiological Factors Pathway Diagram
 YAP1- And WWTR1 (TAZ)-stimulated Gene Expression Pathway antibodies  YAP1- And WWTR1 (TAZ)-stimulated Gene Expression Pathway Diagram
Diseases associated with ANP elisa kitOrgans/Tissues associated with ANP elisa kit
 Disease Name  Pubmed Publications
 Heart Diseases Antibodies  >142 publications with ANP and Heart Diseases
 Heart Failure Antibodies  >87 publications with ANP and Heart Failure
 Hypertension Antibodies  >64 publications with ANP and Hypertension
 Hypertrophy Antibodies  >52 publications with ANP and Hypertrophy
 Cardiomegaly Antibodies  >38 publications with ANP and Cardiomegaly
 Myocardial Ischemia Antibodies  >32 publications with ANP and Myocardial Ischemia
 Kidney Diseases Antibodies  >32 publications with ANP and Kidney Diseases
 Hypertrophy, Left Ventricular Antibodies  >20 publications with ANP and Hypertrophy, Left Ventricular
 Cardiomyopathies Antibodies  >20 publications with ANP and Cardiomyopathies
 Arrhythmias, Cardiac Antibodies  >20 publications with ANP and Arrhythmias, Cardiac
 Organ/Tissue Name  Pubmed Publications
 Heart Antibodies  >206 publications with ANP and Heart
 Brain Antibodies  >50 publications with ANP and Brain
 Lung Antibodies  >25 publications with ANP and Lung
 Embryonic Tissue Antibodies  >19 publications with ANP and Embryonic Tissue
 Connective Tissue Antibodies  >7 publications with ANP and Connective Tissue
 Uterus Antibodies  >2 publications with ANP and Uterus
 Eye Antibodies  >2 publications with ANP and Eye
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