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BCL2 elisa kit :: Rat B-cell CLL/lymphoma 2 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS704498
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (Limit 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $590 +1 FREE 8GB USB
96-Strip-Wells  /  $855 +1 FREE 8GB USB
5x96-Strip-Wells  /  $3,110 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,890 +7 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

B-cell CLL/lymphoma 2 (BCL2), ELISA Kit

 Also Known As   

Rat B-cell CLL/lymphoma 2, BCL2 ELISA Kit

 Product Synonym Names    Rat B-cell CLL/lymphoma 2 (BCL2) ELISA kit; Bcl-2; B-cell lymphoma protein 2; OTTHUMP00000163680; B-cell CLL/lymphoma 2
 Product Gene Name   

BCL2 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS704498 Testing Data    Testing Data PDF
 3D Structure    ModBase 3D Structure for P49950
 Species Reactivity    Rat
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 Specificity    This assay has high sensitivity and excellent specificity for detection of rat BCL2. No significant cross-reactivity or interference between rat BCL2 and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates
 Assay Type    Sandwich
 Detection Range    0.312 ng/ml -20 ng/ml.
 Sensitivity    The minimum detectable dose of rat BCL2 is typically less than 0.078 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of BCL2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for BCL2 purchase    MBS704498 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the B-cell CLL/lymphoma 2 (BCL2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.312 ng/ml -20 ng/ml in biological research samples containing BCL2, with an estimated sensitivity of The minimum detectable dose of rat BCL2 is typically less than 0.078 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. The ELISA analytical biochemical technique of the MBS704498 kit is based on BCL2 antibody-BCL2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect BCL2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, BCL2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for BCL2 elisa kit

   Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for BCL2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any BCL2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for BCL2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of BCL2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of BCL2 elisa kit    BCL2 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS704498. Click to request current manual
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NCBI/Uniprot data below describe general gene information for BCL2. It may not necessarily be applicable to this product.
 NCBI GI #    8392974
 NCBI GeneID    24224
 NCBI Accession #    NP_058689.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_016993.1 [Other Products]
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 UniProt Primary Accession #    P49950 [Other Products]
 UniProt Secondary Accession #    Q62837; Q64032 [Other Products]
 UniProt Related Accession #    P49950 [Other Products]
 Molecular Weight    26,622 Da
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 NCBI Official Full Name    apoptosis regulator Bcl-2
 NCBI Official Synonym Full Names    B-cell CLL/lymphoma 2
 NCBI Official Symbol    Bcl2 [Similar Products]
 NCBI Official Synonym Symbols   
Bcl-2
[Similar Products]
 NCBI Protein Information    apoptosis regulator Bcl-2; Bcl2-like protein; B-cell leukemia/lymphoma 2; B cell lymphoma 2 associated oncogene
 UniProt Protein Name    Apoptosis regulator Bcl-2
 Protein Family    Apoptosis regulator
 UniProt Gene Name    Bcl2 [Similar Products]
 UniProt Synonym Gene Names    Bcl-2 [Similar Products]
 UniProt Entry Name    BCL2_RAT
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 NCBI Summary for BCL2    an anti-apoptotic protein; involved in inhibiting cell death in many different cell types [RGD, Feb 2006]
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 UniProt Comments for BCL2    Function: Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1).
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 Research Articles on BCL2    1. Enhanced espression of Bcl2 is required to sustain oxoglutarate carrier-dependent glutathione transport into the mitochondria.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with BCL2 elisa kitPathways associated with BCL2 elisa kit
 Reference Product  PubMed Publications
 Bax elisa kit  >1313 publications with BCL2 and Bax
 Bad elisa kit  >206 publications with BCL2 and Bad
 Tp53 elisa kit  >125 publications with BCL2 and Tp53
 Bid elisa kit  >103 publications with BCL2 and Bid
 Bak1 elisa kit  >71 publications with BCL2 and Bak1
 Apaf1 elisa kit  >41 publications with BCL2 and Apaf1
 Becn1 elisa kit  >38 publications with BCL2 and Becn1
 Akt1 elisa kit  >34 publications with BCL2 and Akt1
 Bcl2l11 elisa kit  >14 publications with BCL2 and Bcl2l11
 Products by Pathway  Pathway Diagram
 ATM Pathway antibodies  ATM Pathway Diagram
 Adrenergic Signaling In Cardiomyocytes Pathway antibodies  Adrenergic Signaling In Cardiomyocytes Pathway Diagram
 Adrenergic Signaling In Cardiomyocytes Pathway antibodies  Adrenergic Signaling In Cardiomyocytes Pathway Diagram
 Amyotrophic Lateral Sclerosis (ALS) Pathway antibodies  Amyotrophic Lateral Sclerosis (ALS) Pathway Diagram
 Amyotrophic Lateral Sclerosis (ALS) Pathway antibodies  Amyotrophic Lateral Sclerosis (ALS) Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 B Cell Receptor Signaling Pathway antibodies  B Cell Receptor Signaling Pathway Diagram
 Cholinergic Synapse Pathway antibodies  Cholinergic Synapse Pathway Diagram
Diseases associated with BCL2 elisa kitOrgans/Tissues associated with BCL2 elisa kit
 Disease Name  Pubmed Publications
 Lymphoma, Non-Hodgkin Antibodies  >712 publications with BCL2 and Lymphoma, Non-Hodgkin
 Carcinoma Antibodies  >637 publications with BCL2 and Carcinoma
 Lymphoma, Follicular Antibodies  >371 publications with BCL2 and Lymphoma, Follicular
 Necrosis Antibodies  >360 publications with BCL2 and Necrosis
 Lymphoma, Large B-Cell, Diffuse Antibodies  >353 publications with BCL2 and Lymphoma, Large B-Cell, Diffuse
 Breast Neoplasms Antibodies  >302 publications with BCL2 and Breast Neoplasms
 Adenocarcinoma Antibodies  >264 publications with BCL2 and Adenocarcinoma
 Disease Progression Antibodies  >241 publications with BCL2 and Disease Progression
 Cell Transformation, Neoplastic Antibodies  >144 publications with BCL2 and Cell Transformation, Neoplastic
 Leukemia, Lymphocytic, Chronic, B-Cell Antibodies  >142 publications with BCL2 and Leukemia, Lymphocytic, Chronic, B-Cell
 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >13 publications with BCL2 and Bone
 Connective Tissue Antibodies  >4 publications with BCL2 and Connective Tissue
 Heart Antibodies  >3 publications with BCL2 and Heart
 Muscle Antibodies  >3 publications with BCL2 and Muscle
 Brain Antibodies  >3 publications with BCL2 and Brain
 Vascular Antibodies  >2 publications with BCL2 and Vascular
 Embryonic Tissue Antibodies  >2 publications with BCL2 and Embryonic Tissue
 Ear Antibodies  >1 publications with BCL2 and Ear
 Kidney Antibodies  >1 publications with BCL2 and Kidney
 Umbilical Cord Antibodies  >1 publications with BCL2 and Umbilical Cord
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