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BMP-2 elisa kit :: Rat BMP-2 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS355415
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
96 Tests  /  $455 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

BMP-2, ELISA Kit

★Popular Item★
 Also Known As   

BMP-2 Rat ELISA Kit

 Product Synonym Names    Rat BMP-2
 Product Gene Name   

BMP-2 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 Chromosome Location    Chromosome: 20; NC_000020.10 (6748745..6760910). Location: 20p12
 OMIM    112261
 3D Structure    ModBase 3D Structure for P12643
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 Species Reactivity    Rat
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 Assay Type    Sandwich
 Detection Range    62.5 pg/ml-4000 pg/ml
 Sensitivity    < 2pg/ml
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 Preparation and Storage    Store at 2-8 degree C for 4 months, or at -20 degree C for 8 months.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of BMP-2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for BMP-2 purchase    MBS355415 is a ready-to-use microwell, strip-or-full plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the BMP-2, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 62.5 pg/ml-4000 pg/ml in biological research samples containing BMP-2, with an estimated sensitivity of < 2pg/ml. The ELISA analytical biochemical technique of the MBS355415 kit is based on BMP-2 antibody-BMP-2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect BMP-2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, BMP-2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for BMP-2 elisa kit

   Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-BMP-2 polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-BMP-2 polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the BMP-2 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of BMP-2 can be calculated.

Background: Bone morphogenetic protein 2 (BMP-2) belongs to the TGF-beta superfamily of proteins. Like other family members, it plays an important role in the development of bone and cartilage. It has been demonstrated to potently induce osteoblast differentiation in a variety of cell types.BMP-2 has an ability to induce the formation of ectopic cartilage when implanted subcutaneously.
 Applications Tested/Suitable for BMP-2 elisa kit   

Quantitative sELISA (EIA)

 Application Notes for BMP-2 elisa kit    For quantitative detection of BMP-2 in serum, plasma, body fluids, tissue lysates or cell culture supernates.
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 Typical Testing Data/Standard Curve (for reference only) of BMP-2 elisa kit    BMP-2 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS355415. Click to request current manual
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NCBI/Uniprot data below describe general gene information for BMP-2. It may not necessarily be applicable to this product.
 NCBI GI #    6649952
 NCBI GeneID    650
 NCBI Accession #    AAF21646.1 [Other Products]
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 UniProt Primary Accession #    P12643 [Other Products]
 UniProt Related Accession #    P12643 [Other Products]
 Molecular Weight    44,702 Da
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 NCBI Official Full Name    bone morphogenetic protein 2
 NCBI Official Synonym Full Names    bone morphogenetic protein 2
 NCBI Official Symbol    BMP2 [Similar Products]
 NCBI Official Synonym Symbols   
BDA2; BMP2A
[Similar Products]
 NCBI Protein Information    bone morphogenetic protein 2; BMP-2A; bone morphogenetic protein 2A
 UniProt Protein Name    Bone morphogenetic protein 2
 UniProt Synonym Protein Names   
Bone morphogenetic protein 2A
 Protein Family    BMP-2-inducible protein kinase
 UniProt Gene Name    BMP2 [Similar Products]
 UniProt Synonym Gene Names    BMP2A; BMP-2; BMP-2A [Similar Products]
 UniProt Entry Name    BMP2_HUMAN
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 NCBI Summary for BMP-2    The protein encoded by this gene belongs to the transforming growth factor-beta (TGFB) superfamily. The encoded protein acts as a disulfide-linked homodimer and induces bone and cartilage formation. [provided by RefSeq, Jul 2008]
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 UniProt Comments for BMP-2    Function: Induces cartilage and bone formation.

Subunit structure: Homodimer; disulfide-linked. Interacts with SOSTDC1. Interacts with GREM2, RGMA, RGMB and RGMC. Interacts with ASPN

By similarity. Ref.4

Subcellular location: Secreted.

Tissue specificity: Particularly abundant in lung, spleen and colon and in low but significant levels in heart, brain, placenta, liver, skeletal muscle, kidney, pancreas, prostate, ovary and small intestine.

Pharmaceutical use: Available under the name Infuse (Medtronic Sofamor Danek). Used for treating open tibial shaft fractures.

Sequence similarities: Belongs to the TGF-beta family.
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 Research Articles on BMP-2    1. This Bmp2 nsSNP will cause changes in the protein structure.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with BMP-2 elisa kitPathways associated with BMP-2 elisa kit
 Reference Product  PubMed Publications
 BMPR2 elisa kit  >190 publications with BMP-2 and BMPR2
 BMPR1A elisa kit  >164 publications with BMP-2 and BMPR1A
 SMAD4 elisa kit  >86 publications with BMP-2 and SMAD4
 BMPR1B elisa kit  >53 publications with BMP-2 and BMPR1B
 SMAD7 elisa kit  >32 publications with BMP-2 and SMAD7
 NOG elisa kit  >9 publications with BMP-2 and NOG
 ACVR2B elisa kit  >7 publications with BMP-2 and ACVR2B
 GREM2 elisa kit  >5 publications with BMP-2 and GREM2
 CHRD elisa kit  >3 publications with BMP-2 and CHRD
 SOSTDC1 elisa kit  >2 publications with BMP-2 and SOSTDC1
 Products by Pathway  Pathway Diagram
 Adipogenesis Pathway antibodies  Adipogenesis Pathway Diagram
 BDNF Signaling Pathway antibodies  BDNF Signaling Pathway Diagram
 BMP Signalling And Regulation Pathway antibodies  BMP Signalling And Regulation Pathway Diagram
 Basal Cell Carcinoma Pathway antibodies  Basal Cell Carcinoma Pathway Diagram
 Basal Cell Carcinoma Pathway antibodies  Basal Cell Carcinoma Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Elastic Fibre Formation Pathway antibodies  Elastic Fibre Formation Pathway Diagram
 Extracellular Matrix Organization Pathway antibodies  Extracellular Matrix Organization Pathway Diagram
 Heart Development Pathway antibodies  Heart Development Pathway Diagram
Diseases associated with BMP-2 elisa kitOrgans/Tissues associated with BMP-2 elisa kit
 Disease Name  Pubmed Publications
 Bone Diseases Antibodies  >516 publications with BMP-2 and Bone Diseases
 Neoplasms Antibodies  >262 publications with BMP-2 and Neoplasms
 Necrosis Antibodies  >99 publications with BMP-2 and Necrosis
 Inflammation Antibodies  >79 publications with BMP-2 and Inflammation
 Pain Antibodies  >42 publications with BMP-2 and Pain
 Hypertrophy Antibodies  >39 publications with BMP-2 and Hypertrophy
 Hypertension Antibodies  >36 publications with BMP-2 and Hypertension
 Fibrosis Antibodies  >30 publications with BMP-2 and Fibrosis
 Breast Neoplasms Antibodies  >29 publications with BMP-2 and Breast Neoplasms
 Kidney Diseases Antibodies  >24 publications with BMP-2 and Kidney Diseases
 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >3753 publications with BMP-2 and Bone
 Connective Tissue Antibodies  >586 publications with BMP-2 and Connective Tissue
 Embryonic Tissue Antibodies  >422 publications with BMP-2 and Embryonic Tissue
 Heart Antibodies  >175 publications with BMP-2 and Heart
 Brain Antibodies  >147 publications with BMP-2 and Brain
 Kidney Antibodies  >82 publications with BMP-2 and Kidney
 Lung Antibodies  >66 publications with BMP-2 and Lung
 Liver Antibodies  >64 publications with BMP-2 and Liver
 Eye Antibodies  >58 publications with BMP-2 and Eye
 Prostate Antibodies  >25 publications with BMP-2 and Prostate
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