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NGF/NGFbeta elisa kit :: Rat Beta-nerve growth factor ELISA Kit

Scan QR to view Datasheet Catalog #    MBS762165
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $300 +1 FREE 8GB USB
96-Strip-Wells  /  $415 +1 FREE 8GB USB
5x96-Strip-Wells  /  $1,825 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,425 +4 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Beta-nerve growth factor (NGF/NGFbeta), ELISA Kit

★Popular Item★
 Also Known As   

Rat Beta-nerve growth factor ELISA Kit

 Product Synonym Names    NGF/NGFb/NGFB/beta-nerve growth factor/Beta-NGF/HSAN5/nerve growth factor (beta polypeptide); Beta-nerve growth factor
 Product Gene Name   

NGF/NGFbeta elisa kit

[Similar Products]
 Product Synonym Gene Name    NGF/NGFb [Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS762165 COA    COA PDF
 OMIM    162030
 3D Structure    ModBase 3D Structure for P01138
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 Species Reactivity    Rat
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 Specificity    This assay has high sensitivity and excellent specificity for detection of NGF/NGF? . No significant cross-reactivity or interference between NGF/NGF? and analogues was observed. Note: Limited by current skills and knowledge, it is difficult for us to complete the crossreactivity detection between NGF/NGF? and all the analogues, therefore, cross reaction may still exist.
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 Samples    Serum, plasma, tissue homogenates and other biological fluids
 Assay Type    Sandwich
 Detection Range    39.063-2500pg/ml
 Sensitivity    <23.438pg/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level NGF/NGF? were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
 Inter-assay Precision    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level NGF/NGF? were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
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 Preparation and Storage    Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of NGF/NGFbeta elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for NGF/NGFbeta purchase    MBS762165 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Beta-nerve growth factor (NGF/NGFbeta) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 39.063-2500pg/ml in biological research samples containing NGF/NGFbeta, with an estimated sensitivity of <23.438pg/ml. The ELISA analytical biochemical technique of the MBS762165 kit is based on NGF/NGFbeta antibody-NGF/NGFbeta antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect NGF/NGFbeta antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, NGF/NGFbeta. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for NGF/NGFbeta elisa kit

   Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- NGF/NGF? antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- NGF/NGF? antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the NGF/NGF? amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of NGF/NGF? can be calculated.
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 Typical Testing Data/Standard Curve (for reference only) of NGF/NGFbeta elisa kit    NGF/NGFbeta elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS762165. Click to request current manual
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NCBI/Uniprot data below describe general gene information for NGF/NGFbeta. It may not necessarily be applicable to this product.
 NCBI GI #    671883
 NCBI GeneID    4803
 NCBI Accession #    CAA24755.1 [Other Products]
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 UniProt Primary Accession #    P01138 [Other Products]
 UniProt Secondary Accession #    Q6FHA0; Q96P60; Q9P2Q8; Q9UKL8; A1A4E5 [Other Products]
 UniProt Related Accession #    P01138 [Other Products]
 Molecular Weight    26,959 Da
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 NCBI Official Full Name    beta-nerve growth factor
 NCBI Official Synonym Full Names    nerve growth factor (beta polypeptide)
 NCBI Official Symbol    NGF [Similar Products]
 NCBI Official Synonym Symbols   
NGFB; HSAN5; Beta-NGF
[Similar Products]
 NCBI Protein Information    beta-nerve growth factor
 UniProt Protein Name    Beta-nerve growth factor
 Protein Family    Beta-nerve growth factor
 UniProt Gene Name    NGF [Similar Products]
 UniProt Synonym Gene Names    NGFB; Beta-NGF [Similar Products]
 UniProt Entry Name    NGF_HUMAN
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 NCBI Summary for NGF/NGFbeta    This gene is a member of the NGF-beta family and encodes a secreted protein which homodimerizes and is incorporated into a larger complex. This protein has nerve growth stimulating activity and the complex is involved in the regulation of growth and the differentiation of sympathetic and certain sensory neurons. Mutations in this gene have been associated with hereditary sensory and autonomic neuropathy, type 5 (HSAN5), and dysregulation of this gene's expression is associated with allergic rhinitis. [provided by RefSeq, Jul 2008]
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 UniProt Comments for NGF/NGFbeta    NGF: Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades through those receptor tyrosine kinase to regulate neuronal proliferation, differentiation and survival. Homodimer. Belongs to the NGF-beta family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 1p13.1

Cellular Component: cytoplasmic membrane-bound vesicle; endosome; extracellular region; Golgi lumen

Molecular Function: growth factor activity; metalloendopeptidase inhibitor activity; nerve growth factor receptor binding; protein binding; receptor signaling protein activity

Biological Process: activation of MAPKK activity; cell-cell signaling; induction of apoptosis via death domain receptors; negative regulation of apoptosis; negative regulation of cell cycle; negative regulation of neuron apoptosis; nerve growth factor processing; nerve growth factor receptor signaling pathway; neurite morphogenesis; neuron apoptosis; peripheral nervous system development; phosphoinositide-mediated signaling; phospholipase C activation; positive regulation of apoptosis; positive regulation of axon extension; positive regulation of axonogenesis; positive regulation of collateral sprouting; positive regulation of neuron maturation; positive regulation of protein ubiquitination; Ras protein signal transduction; regulation of axonogenesis; regulation of caspase activity; regulation of neuron differentiation; regulation of neurotransmitter secretion; regulation of release of sequestered calcium ion into cytosol; sensory perception of pain; small GTPase mediated signal transduction; transmembrane receptor protein tyrosine kinase signaling pathway

Disease: Neuropathy, Hereditary Sensory And Autonomic, Type V
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 Research Articles on NGF/NGFbeta    1. NGF and its precursor proNGF are primarily considered as regulators of neuronal function that induce their responses via the tyrosine kinase receptor TrkA and the panneurotrophin receptor p75NTR. (Review)
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with NGF/NGFbeta elisa kitPathways associated with NGF/NGFbeta elisa kit
 Reference Product  PubMed Publications
 NGFR elisa kit  >258 publications with NGF/NGFbeta and NGFR
 NTRK1 elisa kit  >51 publications with NGF/NGFbeta and NTRK1
 NTRK2 elisa kit  >19 publications with NGF/NGFbeta and NTRK2
 NGFRAP1 elisa kit  >11 publications with NGF/NGFbeta and NGFRAP1
 PLG elisa kit  >7 publications with NGF/NGFbeta and PLG
 PRDM4 elisa kit  >3 publications with NGF/NGFbeta and PRDM4
 MMP7 elisa kit  >2 publications with NGF/NGFbeta and MMP7
 SORCS3 elisa kit  >1 publications with NGF/NGFbeta and SORCS3
 SORT1 elisa kit  >1 publications with NGF/NGFbeta and SORT1
 Products by Pathway  Pathway Diagram
 ARMS-mediated Activation Pathway antibodies  ARMS-mediated Activation Pathway Diagram
 Activation Of TRKA Receptors Pathway antibodies  Activation Of TRKA Receptors Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Axonal Growth Stimulation Pathway antibodies  Axonal Growth Stimulation Pathway Diagram
 BDNF Signaling Pathway antibodies  BDNF Signaling Pathway Diagram
 Cell Death Signalling Via NRAGE, NRIF And NADE Pathway antibodies  Cell Death Signalling Via NRAGE, NRIF And NADE Pathway Diagram
 Ceramide Signalling Pathway antibodies  Ceramide Signalling Pathway Diagram
 Frs2-mediated Activation Pathway antibodies  Frs2-mediated Activation Pathway Diagram
 Id Signaling Pathway antibodies  Id Signaling Pathway Diagram
Diseases associated with NGF/NGFbeta elisa kitOrgans/Tissues associated with NGF/NGFbeta elisa kit
 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >2369 publications with NGF/NGFbeta and Nervous System Diseases
 Pain Antibodies  >858 publications with NGF/NGFbeta and Pain
 Neurodegenerative Diseases Antibodies  >770 publications with NGF/NGFbeta and Neurodegenerative Diseases
 Inflammation Antibodies  >764 publications with NGF/NGFbeta and Inflammation
 Nerve Degeneration Antibodies  >639 publications with NGF/NGFbeta and Nerve Degeneration
 Peripheral Nervous System Diseases Antibodies  >494 publications with NGF/NGFbeta and Peripheral Nervous System Diseases
 Necrosis Antibodies  >473 publications with NGF/NGFbeta and Necrosis
 Hyperalgesia Antibodies  >340 publications with NGF/NGFbeta and Hyperalgesia
 Heart Diseases Antibodies  >139 publications with NGF/NGFbeta and Heart Diseases
 Memory Disorders Antibodies  >121 publications with NGF/NGFbeta and Memory Disorders
 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >683 publications with NGF/NGFbeta and Muscle
 Embryonic Tissue Antibodies  >624 publications with NGF/NGFbeta and Embryonic Tissue
 Skin Antibodies  >556 publications with NGF/NGFbeta and Skin
 Eye Antibodies  >446 publications with NGF/NGFbeta and Eye
 Lung Antibodies  >185 publications with NGF/NGFbeta and Lung
 Intestine Antibodies  >115 publications with NGF/NGFbeta and Intestine
 Spleen Antibodies  >92 publications with NGF/NGFbeta and Spleen
 Uncharacterized Tissue Antibodies  >1 publications with NGF/NGFbeta and Uncharacterized Tissue
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