Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource rightarrow ELISA Kit rightarrow CA2 rightarrow Rat CA2  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Menu separator
separator
ELISA Kit ELISA Kit
CLIA Kit CLIA Kit
PCR Kit PCR Kit
Monoclonal Antibody Monoclonal Antibody
Polyclonal Antibody Polyclonal Antibody
Secondary Antibody Secondary Antibody
Antigen Antigen
Biochemical Biochemical
cDNA Clone cDNA Clone
siRNA siRNA
Peptide Peptide
Recombinant/Purified Protein Rec./Purified Protein

Custom ELISA Kit Custom ELISA Kit
Custom Protein Custom Protein
Custom Antibody Custom Antibody
Antibody Matched Pairs Antibody Matched Pairs
Antibody & Corresponding Blocking Peptide Pairs Antibody Peptide Pairs
Phospho-Specific Antibodies Phospho Antibodies
Products by Disease Products by Disease
Products by Pathway Products by Pathway
Products by Tissue Products by Tissue

arrow Advanced Search
arrow Submit Technical Q&A
arrow International Distributors
arrow Contact Us
separator
Our Best Sellers moreseparator
separator
 • polymerase (RNA) III (DNA directed) polypeptide D, 44kDa (PO... ELISA Kit
 • Myelin-associated glycoprotein (MAG) Recombinant Protein
 • Calbindin (CALB1) ELISA Kit
 • alpha 1 Antichymotrypsin (SERPINA3) Native Protein
 • Ca2+-calmodulin dependent protein kinase (CaM kinase) ELISA Kit
 • PARP-1 Antibody
 • acid phosphatase-like 2 (ACPL2) ELISA Kit
 • ACTalpha1 (Actin Alpha 1, Skeletal Muscle) (ACTa1) CLIA Kit
 • Disintegrin and metalloproteinase domain-containing protein ... Recombinant Protein
 • Carcinoembryonic Antigen (CEA) Antibody
 • Catechol-O-Metthyhransferas e ELSA (COMT) ELISA Kit
 • PMP2 Recombinant Protein
 • Synuclein Alpha (SNCa) Recombinant Protein
 • GSK-3a-b (Tyr-279-Tyr-216) Antibody
 downarrow more ...
separator
separator
DatasheetFull DatasheetPrinter Friendly DatasheetPrint This DatasheetAdd to Compare ListHave Questions? Ask UsRequest for a Quotation today

CA2 elisa kit :: Rat CA2 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2510418
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $410 +1 FREE 8GB USB
96-Strip-Wells  /  $490 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,040 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,590 +4 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

CA2, ELISA Kit

★Popular Item★
 Also Known As   

Rat CA2 (Carbonic Anhydrase II) ELISA Kit

 Product Gene Name   

CA2 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
Table BarTOPTable Bar
  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS2510418 COA    COA PDF
 OMIM    259730
 3D Structure    ModBase 3D Structure for P00918
Section Bar
 Species Reactivity    Rat
Section Bar
 Specificity    This kit recognizes natural and recombinant Rat CA2. No significant cross-reactivity or interference between Rat CA2 and analogues was observed.
Section Bar
 Samples    Serum, plasma and other biological fluids.
 Assay Type    Sandwich
 Detection Range    2.813-180ng/mL
 Sensitivity    1.688ng/mL
Section Bar
 Preparation and Storage    Store at 4 degree C.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of CA2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for CA2 purchase    MBS2510418 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the CA2, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 2.813-180ng/mL in biological research samples containing CA2, with an estimated sensitivity of 1.688ng/mL. The ELISA analytical biochemical technique of the MBS2510418 kit is based on CA2 antibody-CA2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CA2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CA2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Table BarTOPTable Bar
 

Related Product Information for CA2 elisa kit

   Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Rat CA2 concentrations in serum, plasma and other biological fluids.

Principle of the Assay||This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to CA2. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for CA2 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain CA2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of CA2. You can calculate the concentration of CA2 in the samples by comparing the OD of the samples to the standard curve.
Section Bar
 Typical Testing Data/Standard Curve (for reference only) of CA2 elisa kit    CA2 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Table BarTOPTable Bar
Sample Manual Insert of MBS2510418. Click to request current manual
Table BarTOPTable Bar
NCBI/Uniprot data below describe general gene information for CA2. It may not necessarily be applicable to this product.
 NCBI GI #    49456705
 NCBI GeneID    760
 NCBI Accession #    CAG46673.1 [Other Products]
Section Bar
 UniProt Primary Accession #    P00918 [Other Products]
 UniProt Secondary Accession #    Q6FI12; Q96ET9; B2R7G8 [Other Products]
 UniProt Related Accession #    P00918 [Other Products]
 Molecular Weight    29,246 Da
Table BarTOPTable Bar
 NCBI Official Full Name    CA2, partial
 NCBI Official Synonym Full Names    carbonic anhydrase II
 NCBI Official Symbol    CA2 [Similar Products]
 NCBI Official Synonym Symbols   
CAC; CAII; Car2; CA-II; HEL-76
[Similar Products]
 NCBI Protein Information    carbonic anhydrase 2; carbonate dehydratase II; carbonic anhydrase B; carbonic anhydrase C; carbonic dehydratase; epididymis luminal protein 76
 UniProt Protein Name    Carbonic anhydrase 2
 UniProt Synonym Protein Names   
Carbonate dehydratase II; Carbonic anhydrase C; CAC; Carbonic anhydrase II; CA-II
 Protein Family    Beta-carotene hydroxylase
 UniProt Gene Name    CA2 [Similar Products]
 UniProt Synonym Gene Names    CAC; CA-II [Similar Products]
 UniProt Entry Name    CAH2_HUMAN
Section Bar
 NCBI Summary for CA2    The protein encoded by this gene is one of several isozymes of carbonic anhydrase, which catalyzes reversible hydration of carbon dioxide. Defects in this enzyme are associated with osteopetrosis and renal tubular acidosis. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2014]
Section Bar
 UniProt Comments for CA2    CA2: Essential for bone resorption and osteoclast differentiation. Reversible hydration of carbon dioxide. Can hydrates cyanamide to urea. Involved in the regulation of fluid secretion into the anterior chamber of the eye. Interacts with SLC4A4. Interaction with SLC4A7 regulates SLC4A7 transporter activity. Activated by X-ray, histamine, L-adrenaline, L- and D-phenylalanine, L- and D-histidine, L-His-OMe and beta-Ala- His (carnosine). Competitively inhibited by saccharin, thioxolone, coumarins, 667-coumate, celecoxib (Celebrex), valdecoxib (Bextra), SC-125, SC-560, diclofenac, acetate, azide, bromide, sulfonamide derivatives such as acetazolamide (AZA), methazolamide (MZA), ethoxzolamide (EZA), dichlorophenamide (DCP), brinzolamide, dansylamide, thiabendazole-5-sulfonamide, trifluoromethane sulfonamide and N-hydroxysulfamide, fructose-based sugar sulfamate RWJ-37497, and Foscarnet (phosphonoformate trisodium salt). Repressed strongly by hydrogen sulfide(HS) and weakly by nitrate (NO(3)). Esterase activity weakly reduced by cyanamide. N- hydroxyurea interfers with zinc binding and inhibit activity. Belongs to the alpha-carbonic anhydrase family.

Protein type: Lyase; Energy Metabolism - nitrogen; EC 4.2.1.1

Chromosomal Location of Human Ortholog: 8q22

Cellular Component: extracellular space; microvillus; axon; basolateral plasma membrane; apical part of cell; cytoplasm; plasma membrane; cytosol; myelin sheath

Molecular Function: protein binding; carbonate dehydratase activity; zinc ion binding

Biological Process: secretion; positive regulation of osteoclast differentiation; positive regulation of cellular pH reduction; positive regulation of synaptic transmission, GABAergic; one-carbon compound metabolic process; odontogenesis of dentine-containing teeth; response to estrogen stimulus; bicarbonate transport; response to zinc ion; regulation of intracellular pH; positive regulation of bone resorption; morphogenesis of an epithelium; kidney development; response to pH

Disease: Osteopetrosis, Autosomal Recessive 3
Table BarTOPTable Bar
 Research Articles on CA2    1. Myocardial carbonic anhydrase 1/2 activation is significantly elevated in diabetic ischemic cardiomyopathy.
Table BarTOPTable Bar
 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Table BarTOPTable Bar
Products associated with CA2 elisa kitPathways associated with CA2 elisa kit
 Reference Product  PubMed Publications
 PTH elisa kit  >730 publications with CA2 and PTH
 CALB1 elisa kit  >107 publications with CA2 and CALB1
 STX1A elisa kit  >31 publications with CA2 and STX1A
 SLC4A4 elisa kit  >5 publications with CA2 and SLC4A4
 CALM1 elisa kit  >3 publications with CA2 and CALM1
 SLC4A1 elisa kit  >3 publications with CA2 and SLC4A1
 SLC4A2 elisa kit  >1 publications with CA2 and SLC4A2
 CALM3 elisa kit  >1 publications with CA2 and CALM3
 Products by Pathway  Pathway Diagram
 Bile Secretion Pathway antibodies  Bile Secretion Pathway Diagram
 Bile Secretion Pathway antibodies  Bile Secretion Pathway Diagram
 Collecting Duct Acid Secretion Pathway antibodies  Collecting Duct Acid Secretion Pathway Diagram
 Collecting Duct Acid Secretion Pathway antibodies  Collecting Duct Acid Secretion Pathway Diagram
 Erythrocytes Take Up Carbon Dioxide And Release Oxygen Pathway antibodies  Erythrocytes Take Up Carbon Dioxide And Release Oxygen Pathway Diagram
 Erythrocytes Take Up Oxygen And Release Carbon Dioxide Pathway antibodies  Erythrocytes Take Up Oxygen And Release Carbon Dioxide Pathway Diagram
 Gastric Acid Secretion Pathway antibodies  Gastric Acid Secretion Pathway Diagram
 Gastric Acid Secretion Pathway antibodies  Gastric Acid Secretion Pathway Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Nitrogen Metabolism Pathway antibodies  Nitrogen Metabolism Pathway Diagram
Diseases associated with CA2 elisa kitOrgans/Tissues associated with CA2 elisa kit
 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >6795 publications with CA2 and Cardiovascular Diseases
 Nervous System Diseases Antibodies  >4681 publications with CA2 and Nervous System Diseases
 Heart Diseases Antibodies  >4078 publications with CA2 and Heart Diseases
 Disease Models, Animal Antibodies  >2293 publications with CA2 and Disease Models, Animal
 Hypertension Antibodies  >2291 publications with CA2 and Hypertension
 Necrosis Antibodies  >1382 publications with CA2 and Necrosis
 Inflammation Antibodies  >1376 publications with CA2 and Inflammation
 Hypertrophy Antibodies  >1213 publications with CA2 and Hypertrophy
 Kidney Diseases Antibodies  >785 publications with CA2 and Kidney Diseases
 Fibrosis Antibodies  >601 publications with CA2 and Fibrosis
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >20309 publications with CA2 and Brain
 Blood Antibodies  >17336 publications with CA2 and Blood
 Heart Antibodies  >14545 publications with CA2 and Heart
 Liver Antibodies  >4923 publications with CA2 and Liver
 Kidney Antibodies  >4451 publications with CA2 and Kidney
 Pancreas Antibodies  >3231 publications with CA2 and Pancreas
 Intestine Antibodies  >2484 publications with CA2 and Intestine
 Eye Antibodies  >2454 publications with CA2 and Eye
 Lung Antibodies  >2046 publications with CA2 and Lung
 Embryonic Tissue Antibodies  >1735 publications with CA2 and Embryonic Tissue
Table BarTOPTable Bar
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions