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CCL3 elisa kit :: Rat CCL3 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS824889
 Typical Testing Data/Standard Curve (for reference only)
Unit / Price
96 Tests  /  $470 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

CCL3, ELISA Kit

★Popular Item★
 Also Known As   

Rat CCL3 ELISA Kit

 Product Synonym Names    G0S19-1; MIP1A; SCYA3; C-C motif chemokine 3; G0/G1 switch regulatory protein 19-1; Macrophage inflammatory protein 1-alpha; MIP-1-alpha; PAT 464.1; SIS-beta; Small-inducible cytokine A3; Tonsillar lymphocyte LD78 alpha protein
 Product Gene Name   

CCL3 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 3D Structure    ModBase 3D Structure for P50229
 Species Reactivity    Rat
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 Specificity    The Rat CCL3 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Rat CCL3 proteins within the range of 7.8 pg/ml - 500 pg/ml.
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 Samples    Cell Culture Supernatants, Serum, Plasma
 Assay Type    Quantitative Sandwich
 Sensitivity    <1 pg/ml.
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 Preparation and Storage    Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of CCL3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for CCL3 purchase    MBS824889 is a ready-to-use microwell, strip-or-full plate Quantitative Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the CCL3, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CCL3, with an estimated sensitivity of <1 pg/ml. The ELISA analytical biochemical technique of the MBS824889 kit is based on CCL3 antibody-CCL3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CCL3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CCL3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Cell Culture Supernatants, Serum, Plasma. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for CCL3 elisa kit

   Principle of the Assay: MBS Rat CCL3 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat CCL3 in Cell Culture Supernatants, Serum, Plasma. This assay employs an antibody specific for Rat CCL3 coated on a 96-well plate. Standards and samples are pipetted into the wells and CCL3 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Rat CCL3 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CCL3 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Background: Macrophage inflammatory protein-1 alpha (MIP-1 alpha), also called CCL3, LD78. The cDNA for MIP-1 alpha predicts a mature peptide of 69 amino acids with a molecular mass of 7,889 daltons. LD78 is a member of a newly identified superfamily of small inducible proteins involved in inflammatory responses, wound healing and tumorigenesis. MIP-1 alpha is a chemokine that has pro-inflammatory and stem cell inhibitory activities in vitro. It constitutes an important second signal for mast cell degranulation in the conjunctiva in vivo and consequently for acute-phase disease.
 Applications Tested/Suitable for CCL3 elisa kit   

Sandwich ELISA (SE)

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  Typical Testing Data/Standard Curve (for reference only) of CCL3 elisa kit    CCL3 elisa kit  Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS824889. Click to request current manual
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NCBI/Uniprot data below describe general gene information for CCL3. It may not necessarily be applicable to this product.
 NCBI GI #    40254794
 NCBI GeneID    25542
 NCBI Accession #    NP_037157.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_013025.2 [Other Products]
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 UniProt Primary Accession #    P50229 [Other Products]
 UniProt Secondary Accession #    A1EC87 [Other Products]
 UniProt Related Accession #    P50229 [Other Products]
 Molecular Weight    10,335 Da
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 NCBI Official Full Name    C-C motif chemokine 3
 NCBI Official Synonym Full Names    chemokine (C-C motif) ligand 3
 NCBI Official Symbol    Ccl3 [Similar Products]
 NCBI Official Synonym Symbols   
Scya3; MIP-1a
[Similar Products]
 NCBI Protein Information    C-C motif chemokine 3; MIP-1-alpha; small inducible cytokine A3; small-inducible cytokine A3; macrophage inflammatory protein 1-alpha; Macrophage inflammatory protein 1 alpha (Small inducible cytokine A3)
 UniProt Protein Name    C-C motif chemokine 3
 UniProt Synonym Protein Names   
Macrophage inflammatory protein 1-alpha; MIP-1-alpha; Small-inducible cytokine A3
 Protein Family    C-C motif chemokine
 UniProt Gene Name    Ccl3 [Similar Products]
 UniProt Synonym Gene Names    Mip1a; Scya3; MIP-1-alpha [Similar Products]
 UniProt Entry Name    CCL3_RAT
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 NCBI Summary for CCL3    mediates monocyte and neutrophil chemotaxis; may play a role in the pathogenesis of acute lung injury [RGD, Feb 2006]
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 UniProt Comments for CCL3    Monokine with inflammatory and chemokinetic properties. Has chemotactic activity for monocytes, neutrophils, eosinophils, basophils, and lymphocytes. Required for lung TNF-alpha production, neutrophil recruitment and subsequent lung injury and may function as an autocrine mediator for the macrophage production of TNF-alpha which in turn up-regulates vascular adhesion molecules required for neutrophil influx. This protein binds heparin.
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 Research Articles on CCL3    1. Expression levels of CCL3 in the spinal cord are up-regulated following nerve injury.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with CCL3 elisa kitDiseases associated with CCL3 elisa kit
 Products by Pathway  Pathway Diagram
 Chagas Disease (American Trypanosomiasis) Pathway antibodies  Chagas Disease (American Trypanosomiasis) Pathway Diagram
 Chagas Disease (American Trypanosomiasis) Pathway antibodies  Chagas Disease (American Trypanosomiasis) Pathway Diagram
 Chemokine Receptors Bind Chemokines Pathway antibodies  Chemokine Receptors Bind Chemokines Pathway Diagram
 Chemokine Signaling Pathway antibodies  Chemokine Signaling Pathway Diagram
 Chemokine Signaling Pathway antibodies  Chemokine Signaling Pathway Diagram
 Class A/1 (Rhodopsin-like Receptors) Pathway antibodies  Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 GPCR Ligand Binding Pathway antibodies  GPCR Ligand Binding Pathway Diagram
 Peptide Ligand-binding Receptors Pathway antibodies  Peptide Ligand-binding Receptors Pathway Diagram
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >876 publications with CCL3 and Inflammation
 Necrosis Antibodies  >495 publications with CCL3 and Necrosis
 Disease Models, Animal Antibodies  >308 publications with CCL3 and Disease Models, Animal
 Lung Diseases Antibodies  >233 publications with CCL3 and Lung Diseases
 HIV Infections Antibodies  >187 publications with CCL3 and HIV Infections
 Cardiovascular Diseases Antibodies  >168 publications with CCL3 and Cardiovascular Diseases
 Hypersensitivity Antibodies  >105 publications with CCL3 and Hypersensitivity
 Liver Diseases Antibodies  >92 publications with CCL3 and Liver Diseases
 Fibrosis Antibodies  >77 publications with CCL3 and Fibrosis
 Pneumonia Antibodies  >71 publications with CCL3 and Pneumonia
Organs/Tissues associated with CCL3 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Connective Tissue Antibodies  >40 publications with CCL3 and Connective Tissue
 Adipose Tissue Antibodies  >22 publications with CCL3 and Adipose Tissue
 Inner Ear Antibodies  >1 publications with CCL3 and Inner Ear
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