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CDH5 elisa kit :: Rat Cadherin 5 (CDH5) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2019699 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells  /  $270 +1 FREE 8GB USB
48-Strip-Wells  /  $505 +1 FREE 8GB USB
96-Strip-Wells  /  $685 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,775 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,035 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Cadherin 5 (CDH5), ELISA Kit

★Popular Item★
 Also Known As   

Cadherin 5 (CDH5) ELISA Kit

 Product Synonym Names    CD144; 7B4; Cadherin 5 Type 2; VE-Cadherin; Cadherin,Vascular Endothelial
 Product Gene Name   

CDH5 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 3D Structure    ModBase 3D Structure for P55284
 Species Reactivity    Rat
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Cadherin 5 (CDH5).
No significant cross-reactivity or interference between Cadherin 5 (CDH5) and analogues was observed.
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 Assay Type    Double-antibody Sandwich
 Samples    Serum, Plasma, Tissue homogenates, Cell lysates and Other Biological Fluids
 Detection Range    78-5000pg/mL
 Sensitivity    < 27pg/mL
 Application    Enzyme-linked immunosorbent assay for Antigen Detection.
 Intra-assay Precision (Precision within an assay)    3 samples with low, middle and high level Cadherin 5 (CDH5) were tested 20 times on one plate, respectively.
 Inter-assay Precision (Precision between assays)    3 samples with low, middle and high level Cadherin 5 (CDH5) were tested on 3 different plates, 8 replicates in each plate.
 CV(%) =    SD/meanX100
 Intra-Assay    CV<10%
 Inter-Assay    CV<12%
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 Preparation and Storage    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of CDH5 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for CDH5 purchase    MBS2019699 is a ready-to-use microwell, strip plate Double-antibody Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Cadherin 5 (CDH5) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 78-5000pg/mL in biological research samples containing CDH5, with an estimated sensitivity of < 27pg/mL. The ELISA analytical biochemical technique of the MBS2019699 kit is based on CDH5 antibody-CDH5 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CDH5 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CDH5. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma, Tissue homogenates, Cell lysates and Other Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for CDH5 elisa kit

   The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cadherin 5 (CDH5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cadherin 5 (CDH5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cadherin 5 (CDH5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cadherin 5 (CDH5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
 Product Categories/Family for CDH5 elisa kit    CD & Adhesion molecule; Tumor immunity; Infection immunity
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 Typical Testing Data/Standard Curve (for reference only) of CDH5 elisa kit    CDH5 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2019699. Click to request current manual
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NCBI/Uniprot data below describe general gene information for CDH5. It may not necessarily be applicable to this product.
 NCBI GI #    32451759
 NCBI GeneID    12562
 NCBI Accession #    AAH54790.1 [Other Products]
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 UniProt Primary Accession #    P55284 [Other Products]
 UniProt Secondary Accession #    O35542 [Other Products]
 UniProt Related Accession #    P55284 [Other Products]
 Molecular Weight    87,903 Da
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 NCBI Official Full Name    Cadherin 5
 NCBI Official Synonym Full Names    cadherin 5
 NCBI Official Symbol    Cdh5 [Similar Products]
 NCBI Official Synonym Symbols   
7B4; Vec; VECD; Cd144; VEcad; VE-Cad; AA408225
[Similar Products]
 NCBI Protein Information    cadherin-5
 UniProt Protein Name    Cadherin-5
 UniProt Synonym Protein Names   
Vascular endothelial cadherin; VE-cadherin; CD_antigen: CD144
 Protein Family    Cadherin
 UniProt Gene Name    Cdh5 [Similar Products]
 UniProt Synonym Gene Names    VE-cadherin [Similar Products]
 UniProt Entry Name    CADH5_MOUSE
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 UniProt Comments for CDH5    CDH5: Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. This cadherin may play a important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. It associates with alpha-catenin forming a link to the cytoskeleton. Acts in concert with KRIT1 to establish and maintain correct endothelial cell polarity and vascular lumen. These effects are mediated by recruitment and activation of the Par polarity complex and RAP1B. Required for activation of PRKCZ and for the localization of phosphorylated PRKCZ, PARD3, TIAM1 and RAP1B to the cell junction. Interacts via cadherin 5 domain with PTPRB. Interacts with TRPC4. Interacts with KRIT1. Endothelial tissues and brain.

Protein type: Membrane protein, integral; Motility/polarity/chemotaxis; Cell adhesion

Cellular Component: tight junction; adherens junction; membrane; integral to membrane; plasma membrane; intercellular junction; cell junction

Molecular Function: protein binding; metal ion binding; beta-catenin binding; calcium ion binding; protein phosphatase binding; receptor binding

Biological Process: negative regulation of cell proliferation; cell-cell adhesion; homophilic cell adhesion; cell adhesion; blood vessel maturation
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 Research Articles on CDH5    1. iPS cell-derived Flk1(+)VE-cadherin(+) cells expressing the Er71 are as angiogenic as mES cell-derived cells and incorporate into CD31(+) neovessels.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with CDH5 elisa kitPathways associated with CDH5 elisa kit
 Reference Product  PubMed Publications
 Pecam1 elisa kit  >8 publications with CDH5 and Pecam1
 Cdh1 elisa kit  >4 publications with CDH5 and Cdh1
 Cdh3 elisa kit  >2 publications with CDH5 and Cdh3
 Cdh2 elisa kit  >2 publications with CDH5 and Cdh2
 Ctnnb1 elisa kit  >2 publications with CDH5 and Ctnnb1
 Cdh4 elisa kit  >1 publications with CDH5 and Cdh4
 Ctnna1 elisa kit  >1 publications with CDH5 and Ctnna1
 Products by Pathway  Pathway Diagram
 Adherens Junctions Interactions Pathway antibodies  Adherens Junctions Interactions Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Cell Junction Organization Pathway antibodies  Cell Junction Organization Pathway Diagram
 Cell-Cell Communication Pathway antibodies  Cell-Cell Communication Pathway Diagram
 Cell-cell Junction Organization Pathway antibodies  Cell-cell Junction Organization Pathway Diagram
 Leukocyte Transendothelial Migration Pathway antibodies  Leukocyte Transendothelial Migration Pathway Diagram
 Leukocyte Transendothelial Migration Pathway antibodies  Leukocyte Transendothelial Migration Pathway Diagram
 Signal Transduction Pathway antibodies  Signal Transduction Pathway Diagram
 Signaling By VEGF Pathway antibodies  Signaling By VEGF Pathway Diagram
Diseases associated with CDH5 elisa kitOrgans/Tissues associated with CDH5 elisa kit
 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >8 publications with CDH5 and Cardiovascular Diseases
 Inflammation Antibodies  >4 publications with CDH5 and Inflammation
 Breast Neoplasms Antibodies  >4 publications with CDH5 and Breast Neoplasms
 Hemorrhage Antibodies  >2 publications with CDH5 and Hemorrhage
 Lung Diseases Antibodies  >2 publications with CDH5 and Lung Diseases
 Necrosis Antibodies  >2 publications with CDH5 and Necrosis
 Heart Diseases Antibodies  >2 publications with CDH5 and Heart Diseases
 Atherosclerosis Antibodies  >2 publications with CDH5 and Atherosclerosis
 Colorectal Neoplasms Antibodies  >2 publications with CDH5 and Colorectal Neoplasms
 Myocardial Infarction Antibodies  >2 publications with CDH5 and Myocardial Infarction
 Organ/Tissue Name  Pubmed Publications
 Other Antibodies  >12 publications with CDH5 and Other
 Embryonic Tissue Antibodies  >10 publications with CDH5 and Embryonic Tissue
 Brain Antibodies  >6 publications with CDH5 and Brain
 Heart Antibodies  >4 publications with CDH5 and Heart
 Kidney Antibodies  >4 publications with CDH5 and Kidney
 Lung Antibodies  >3 publications with CDH5 and Lung
 Lymph Node Antibodies  >1 publications with CDH5 and Lymph Node
 Pancreas Antibodies  >1 publications with CDH5 and Pancreas
 Eye Antibodies  >1 publications with CDH5 and Eye
 Mammary Gland Antibodies  >1 publications with CDH5 and Mammary Gland
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