View our list of available Coronavirus (COVID-19, SARS-CoV-2, 2019-nCOV) antibodies, recombinant proteins, ELISA Kits... Options Full Datasheet Printer Friendly Datasheet Prepare for Printing Full Datasheet Printer Friendly Datasheet Prepare for Printing Have Questions? Ask Us! Request A Quote Today! Add To Compare List CDH5 elisa kit :: Rat Cadherin 5 (CDH5) ELISA Kit Catalog # MBS2019699 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment) Unit / Price 24-Strip-Wells / $375 +1 FREE 8GB USB 48-Strip-Wells / $565 +1 FREE 8GB USB 96-Strip-Wells / $750 +1 FREE 8GB USB 5x96-Strip-Wells / $3,040 +3 FREE 8GB USB 10x96-Strip-Wells / $5,565 +6 FREE 8GB USB Product Name Product Info Accession #s Product Desc Diseases/Tissues/Pathways Applications References Product Name Cadherin 5 (CDH5), ELISA Kit Popular Item Full Product Name Rat Cadherin 5 (CDH5) ELISA Kit Product Synonym Names CD144; 7B4; Cadherin 5 Type 2; VE-Cadherin; Cadherin,Vascular Endothelial Product Gene Name CDH5 elisa kit[Similar Products] Research Use Only For Research Use Only. Not for use in diagnostic procedures. TOP Sample Manual Insert Download Sample PDF Manual View Sample PDF Manual 3D Structure ModBase 3D Structure for P55284 Species Reactivity Rat Specificity This assay has high sensitivity and excellent specificity for detection of Cadherin 5 (CDH5). No significant cross-reactivity or interference between Cadherin 5 (CDH5) and analogues was observed. Assay Type Double-antibody Sandwich Samples Serum, Plasma, Tissue homogenates, Cell lysates and other biological fluids Detection Range 15.6-1,000ng/mL Sensitivity 6.3ng/mL Application Enzyme-linked immunosorbent assay for Antigen Detection. Intra-assay Precision (Precision within an assay) 3 samples with low, middle and high level Cadherin 5 (CDH5) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) 3 samples with low, middle and high level Cadherin 5 (CDH5) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay CV<10% Inter-Assay CV<12% Preparation and Storage The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end. ISO Certification Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory. Supply Chain Verification Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire. Product Note Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details. Other Notes Small volumes of CDH5 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply. Searchable Terms for CDH5 purchase MBS2019699 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Cadherin 5 (CDH5) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CDH5. The ELISA analytical biochemical technique of the MBS2019699 kit is based on CDH5 antibody-CDH5 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CDH5 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CDH5. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%). TOP Related Product Information for CDH5 elisa kit The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cadherin 5 (CDH5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cadherin 5 (CDH5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cadherin 5 (CDH5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cadherin 5 (CDH5) in the samples is then determined by comparing the O.D. of the samples to the standard curve. Product Categories/Family for CDH5 elisa kit CD & Adhesion molecule; Tumor immunity; Infection immunity Typical Testing Data/Standard Curve (for reference only) of CDH5 elisa kit TOP Sample Manual Insert of MBS2019699. NCBI/Uniprot data below describe general gene information for CDH5. It may not necessarily be applicable to this product. NCBI GI # 32451759 NCBI GeneID 12562 NCBI Accession # AAH54790.1 [Other Products] UniProt Primary Accession # P55284 [Other Products] UniProt Secondary Accession # O35542[Other Products] UniProt Related Accession # P55284[Other Products] Molecular Weight 87,903 Da TOP NCBI Official Full Name Cadherin 5 NCBI Official Synonym Full Names cadherin 5 NCBI Official Symbol Cdh5 [Similar Products] NCBI Official Synonym Symbols 7B4; Vec; VECD; Cd144; VEcad; VE-Cad; AA408225 [Similar Products] NCBI Protein Information cadherin-5 UniProt Protein Name Cadherin-5 UniProt Synonym Protein Names Vascular endothelial cadherin; VE-cadherin; CD_antigen: CD144 Protein Family Cadherin UniProt Gene Name Cdh5 [Similar Products] UniProt Synonym Gene Names VE-cadherin [Similar Products] UniProt Entry Name CADH5_MOUSE TOP TOP UniProt Comments for CDH5 CDH5: Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. This cadherin may play a important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. It associates with alpha-catenin forming a link to the cytoskeleton. Acts in concert with KRIT1 to establish and maintain correct endothelial cell polarity and vascular lumen. These effects are mediated by recruitment and activation of the Par polarity complex and RAP1B. Required for activation of PRKCZ and for the localization of phosphorylated PRKCZ, PARD3, TIAM1 and RAP1B to the cell junction. Interacts via cadherin 5 domain with PTPRB. Interacts with TRPC4. Interacts with KRIT1. Endothelial tissues and brain.Protein type: Membrane protein, integral; Motility/polarity/chemotaxis; Cell adhesionCellular Component: tight junction; adherens junction; membrane; integral to membrane; plasma membrane; intercellular junction; cell junctionMolecular Function: protein binding; metal ion binding; beta-catenin binding; calcium ion binding; protein phosphatase binding; receptor bindingBiological Process: negative regulation of cell proliferation; cell-cell adhesion; homophilic cell adhesion; cell adhesion; blood vessel maturation Research Articles on CDH5 1. iPS cell-derived Flk1(+)VE-cadherin(+) cells expressing the Er71 are as angiogenic as mES cell-derived cells and incorporate into CD31(+) neovessels. TOP Precautions All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines. Disclaimer While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information. TOP Products associated with CDH5 elisa kit Reference Product PubMed Publications Pecam1 elisa kit >8 publications with CDH5 and Pecam1 Cdh1 elisa kit >4 publications with CDH5 and Cdh1 Cdh3 elisa kit >2 publications with CDH5 and Cdh3 Cdh2 elisa kit >2 publications with CDH5 and Cdh2 Ctnnb1 elisa kit >2 publications with CDH5 and Ctnnb1 Cdh4 elisa kit >1 publications with CDH5 and Cdh4 Ctnna1 elisa kit >1 publications with CDH5 and Ctnna1 Pathways associated with CDH5 elisa kit Products by Pathway Pathway Diagram Adherens Junctions Interactions Pathway antibodies Adherens Junctions Interactions Pathway Diagram Cell Adhesion Molecules (CAMs) Pathway antibodies Cell Adhesion Molecules (CAMs) Pathway Diagram Cell Adhesion Molecules (CAMs) Pathway antibodies Cell Adhesion Molecules (CAMs) Pathway Diagram Cell Junction Organization Pathway antibodies Cell Junction Organization Pathway Diagram Cell-Cell Communication Pathway antibodies Cell-Cell Communication Pathway Diagram Cell-cell Junction Organization Pathway antibodies Cell-cell Junction Organization Pathway Diagram Leukocyte Transendothelial Migration Pathway antibodies Leukocyte Transendothelial Migration Pathway Diagram Leukocyte Transendothelial Migration Pathway antibodies Leukocyte Transendothelial Migration Pathway Diagram Signal Transduction Pathway antibodies Signal Transduction Pathway Diagram Signaling By VEGF Pathway antibodies Signaling By VEGF Pathway Diagram Diseases associated with CDH5 elisa kit Disease Name Pubmed Publications Cardiovascular Diseases Antibodies >8 publications with CDH5 and Cardiovascular Diseases Inflammation Antibodies >4 publications with CDH5 and Inflammation Breast Neoplasms Antibodies >4 publications with CDH5 and Breast Neoplasms Hemorrhage Antibodies >2 publications with CDH5 and Hemorrhage Lung Diseases Antibodies >2 publications with CDH5 and Lung Diseases Necrosis Antibodies >2 publications with CDH5 and Necrosis Heart Diseases Antibodies >2 publications with CDH5 and Heart Diseases Atherosclerosis Antibodies >2 publications with CDH5 and Atherosclerosis Colorectal Neoplasms Antibodies >2 publications with CDH5 and Colorectal Neoplasms Myocardial Infarction Antibodies >2 publications with CDH5 and Myocardial Infarction Organs/Tissues associated with CDH5 elisa kit Organ/Tissue Name Pubmed Publications Other Antibodies >12 publications with CDH5 and Other Embryonic Tissue Antibodies >10 publications with CDH5 and Embryonic Tissue Brain Antibodies >6 publications with CDH5 and Brain Heart Antibodies >4 publications with CDH5 and Heart Kidney Antibodies >4 publications with CDH5 and Kidney Lung Antibodies >3 publications with CDH5 and Lung Lymph Node Antibodies >1 publications with CDH5 and Lymph Node Pancreas Antibodies >1 publications with CDH5 and Pancreas Eye Antibodies >1 publications with CDH5 and Eye Mammary Gland Antibodies >1 publications with CDH5 and Mammary Gland TOP © 2006-24 MyBioSource.com. 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