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CTNT elisa kit :: Rat Cardiac Troponin T ELISA Kit

Scan QR to view Datasheet Catalog #    MBS039759 CTNT elisa kit
Unit / Price
48-Strip-Wells  /  $435 +1 FREE 8GB USB
96-Strip-Wells  /  $600 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,650 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,205 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Cardiac Troponin T (CTNT), ELISA Kit

★Popular Item★
 Also Known As   

Rat Cardiac Troponin T ELISA Kit

 Product Gene Name   

CTNT elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 Chromosome Location    Chromosome: 1; NC_000001.10 (201328142..201346805, complement). Location: 1q32
 OMIM    115195
 3D Structure    ModBase 3D Structure for P45379
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 Species Reactivity    Rat
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 Specificity    No significant cross-reactivity or interference between Rat CTNT and analogues was observed.
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 Samples    Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids
 Assay Type    Sandwich
 Detection Range    50 pg/ml - 1600 pg/ml.
 Sensitivity    10 pg/ml.
 Intended Uses    This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated CTNT concentrations in Rat serum, plasma and other body fluids. Using Purified Rat CTNT antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add CTNT and CTNT antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of CTNT in the samples is then determined by comparing the O.D. of the samples to the standard curve.
 Intra-assay Precision    Intra-assay CV (%) is less than 15%.
 Inter-assay Precision    Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean 100]
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 Preparation and Storage    Store all reagents at 2-8 degree C
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of CTNT elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for CTNT purchase    MBS039759 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Cardiac Troponin T (CTNT) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 50 pg/ml - 1600 pg/ml in biological research samples containing CTNT, with an estimated sensitivity of 10 pg/ml. The ELISA analytical biochemical technique of the MBS039759 kit is based on CTNT antibody-CTNT antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CTNT antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CTNT. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for CTNT elisa kit

   Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated CTNT concentrations in Rat serum, plasma and other body fluids. Using Purified Rat CTNT antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add CTNT and CTNT antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of CTNT in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Sample Manual Insert of MBS039759. Click to request current manual
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NCBI/Uniprot data below describe general gene information for CTNT. It may not necessarily be applicable to this product.
 NCBI GI #    15290516
 NCBI GeneID    7139
 NCBI Accession #    AAK92231.1 [Other Products]
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 UniProt Primary Accession #    P45379 [Other Products]
 UniProt Secondary Accession #    O60214; Q99596; Q99597; Q9BUF6; Q9UM96; A2TDB9; A8K3K6 [Other Products]
 UniProt Related Accession #    P45379 [Other Products]
 Molecular Weight    35,924 Da
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 NCBI Official Full Name    cardiac troponin T
 NCBI Official Synonym Full Names    troponin T type 2 (cardiac)
 NCBI Official Symbol    TNNT2 [Similar Products]
 NCBI Official Synonym Symbols   
CMH2; RCM3; TnTC; cTnT; CMPD2; LVNC6
[Similar Products]
 NCBI Protein Information    troponin T, cardiac muscle; troponin T2, cardiac; cardiomyopathy, hypertrophic 2; cardiomyopathy, dilated 1D (autosomal dominant)
 UniProt Protein Name    Troponin T, cardiac muscle
 UniProt Synonym Protein Names   
Cardiac muscle troponin T
 UniProt Gene Name    TNNT2 [Similar Products]
 UniProt Synonym Gene Names    TnTc; cTnT [Similar Products]
 UniProt Entry Name    TNNT2_HUMAN
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 NCBI Summary for CTNT    The protein encoded by this gene is the tropomyosin-binding subunit of the troponin complex, which is located on the thin filament of striated muscles and regulates muscle contraction in response to alterations in intracellular calcium ion concentration. Mutations in this gene have been associated with familial hypertrophic cardiomyopathy as well as with dilated cardiomyopathy. Transcripts for this gene undergo alternative splicing that results in many tissue-specific isoforms, however, the full-length nature of some of these variants has not yet been determined. [provided by RefSeq, Jul 2008]
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 UniProt Comments for CTNT    TNNT2: Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. Heart. The fetal heart shows a greater expression in the atrium than in the ventricle, while the adult heart shows a greater expression in the ventricle than in the atrium. Isoform 6 predominates in normal adult heart. Isoforms 1, 7 and 8 are expressed in fetal heart. Isoform 7 is also expressed in failing adult heart. Belongs to the troponin T family. 11 isoforms of the human protein are produced by alternative splicing.

Protein type: Motor; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 1q32

Cellular Component: sarcomere; troponin complex; cytosol; striated muscle thin filament

Molecular Function: troponin C binding; structural constituent of cytoskeleton; troponin I binding; ATPase activity; tropomyosin binding; actin binding

Biological Process: regulation of muscle contraction; metabolic process; atrial cardiac muscle morphogenesis; positive regulation of ATPase activity; ventricular cardiac muscle morphogenesis; sarcomere organization; response to calcium ion; negative regulation of ATPase activity; regulation of heart contraction; muscle filament sliding

Disease: Cardiomyopathy, Familial Restrictive, 3; Cardiomyopathy, Dilated, 1d; Cardiomyopathy, Familial Hypertrophic, 2
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 Research Articles on CTNT    1. Using the novel hsTnT assay, the current study provides first evidence that hsTnT levels increase in pulmonary arterial hypertension patients after maximal physical exercise.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with CTNT elisa kitPathways associated with CTNT elisa kit
 Reference Product  PubMed Publications
 MYBPC3 elisa kit  >63 publications with CTNT and MYBPC3
 TNNI3 elisa kit  >49 publications with CTNT and TNNI3
 TPM1 elisa kit  >32 publications with CTNT and TPM1
 MYL2 elisa kit  >18 publications with CTNT and MYL2
 TNNC1 elisa kit  >12 publications with CTNT and TNNC1
 DES elisa kit  >6 publications with CTNT and DES
 TTN elisa kit  >6 publications with CTNT and TTN
 TNNI2 elisa kit  >1 publications with CTNT and TNNI2
 TNNI1 elisa kit  >1 publications with CTNT and TNNI1
 Products by Pathway  Pathway Diagram
 Cardiac Progenitor Differentiation Pathway antibodies  Cardiac Progenitor Differentiation Pathway Diagram
 Cardiac Muscle Contraction Pathway antibodies  Cardiac Muscle Contraction Pathway Diagram
 Cardiac Muscle Contraction Pathway antibodies  Cardiac Muscle Contraction Pathway Diagram
 Dilated Cardiomyopathy Pathway antibodies  Dilated Cardiomyopathy Pathway Diagram
 Dilated Cardiomyopathy Pathway antibodies  Dilated Cardiomyopathy Pathway Diagram
 Hypertrophic Cardiomyopathy (HCM) Pathway antibodies  Hypertrophic Cardiomyopathy (HCM) Pathway Diagram
 Hypertrophic Cardiomyopathy (HCM) Pathway antibodies  Hypertrophic Cardiomyopathy (HCM) Pathway Diagram
 Muscle Contraction Pathway antibodies  Muscle Contraction Pathway Diagram
 Striated Muscle Contraction Pathway antibodies  Striated Muscle Contraction Pathway Diagram
 Striated Muscle Contraction Pathway antibodies  Striated Muscle Contraction Pathway Diagram
Diseases associated with CTNT elisa kitOrgans/Tissues associated with CTNT elisa kit
 Disease Name  Pubmed Publications
 Heart Diseases Antibodies  >126 publications with CTNT and Heart Diseases
 Cardiovascular Diseases Antibodies  >126 publications with CTNT and Cardiovascular Diseases
 Cardiomyopathies Antibodies  >100 publications with CTNT and Cardiomyopathies
 Cardiomyopathy, Hypertrophic Antibodies  >94 publications with CTNT and Cardiomyopathy, Hypertrophic
 Cardiomyopathy, Hypertrophic, Familial Antibodies  >46 publications with CTNT and Cardiomyopathy, Hypertrophic, Familial
 Cardiomyopathy, Dilated Antibodies  >37 publications with CTNT and Cardiomyopathy, Dilated
 Hypertrophy Antibodies  >36 publications with CTNT and Hypertrophy
 Cardiomyopathy, Familial Hypertrophic, 2 Antibodies  >28 publications with CTNT and Cardiomyopathy, Familial Hypertrophic, 2
 Heart Failure Antibodies  >27 publications with CTNT and Heart Failure
 Myocardial Ischemia Antibodies  >12 publications with CTNT and Myocardial Ischemia
 Organ/Tissue Name  Pubmed Publications
 Heart Antibodies  >98 publications with CTNT and Heart
 Muscle Antibodies  >85 publications with CTNT and Muscle
 Blood Antibodies  >38 publications with CTNT and Blood
 Embryonic Tissue Antibodies  >12 publications with CTNT and Embryonic Tissue
 Kidney Antibodies  >6 publications with CTNT and Kidney
 Lung Antibodies  >4 publications with CTNT and Lung
 Brain Antibodies  >4 publications with CTNT and Brain
 Connective Tissue Antibodies  >2 publications with CTNT and Connective Tissue
 Liver Antibodies  >2 publications with CTNT and Liver
 Eye Antibodies  >1 publications with CTNT and Eye
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