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F10 elisa kit :: Rat Coagulation Factor X (F10) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS266437
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $275 +1 FREE 8GB USB
96-Strip-Wells  /  $435 +1 FREE 8GB USB
5x96-Strip-Wells  /  $1,755 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,150 +3 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Coagulation Factor X (F10), ELISA Kit

★Popular Item★
 Also Known As   

Rat Coagulation Factor X (F10) ELISA Kit

 Product Gene Name   

F10 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    613872
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 Species Reactivity    Rat
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 Specificity    No cross-reaction with other factors.
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 Samples    Rat serum, plasma or cell culture supernatant
 Detection Range    20 ng/ml-0.312 ng/ml
 Sensitivity    0.06 ng/mL
 Intra-assay Precision    ? 8%
 Inter-assay Precision    ? 12%
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of F10 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for F10 purchase    MBS266437 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Coagulation Factor X (F10) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 20 ng/ml-0.312 ng/ml in biological research samples containing F10, with an estimated sensitivity of 0.06 ng/mL. The ELISA analytical biochemical technique of the MBS266437 kit is based on F10 antibody-F10 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect F10 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, F10. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Rat serum, plasma or cell culture supernatant. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for F10 elisa kit

   Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Rat F10 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
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 Typical Testing Data/Standard Curve (for reference only) of F10 elisa kit    F10 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS266437. Click to request current manual
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NCBI/Uniprot data below describe general gene information for F10. It may not necessarily be applicable to this product.
 NCBI GI #    182390
 NCBI GeneID    2159
 NCBI Accession #    AAA52421.1 [Other Products]
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 UniProt Secondary Accession #    Q14340 [Other Products]
 UniProt Related Accession #    P00742 [Other Products]
 Molecular Weight    54,732 Da
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 NCBI Official Full Name    coagulation factor X
 NCBI Official Synonym Full Names    coagulation factor X
 NCBI Official Symbol    F10 [Similar Products]
 NCBI Official Synonym Symbols   
FX; FXA
[Similar Products]
 NCBI Protein Information    coagulation factor X; factor Xa; prothrombinase; Stuart-Prower factor
 UniProt Protein Name    Coagulation factor X
 UniProt Synonym Protein Names   
Stuart factor; Stuart-Prower factorCleaved into the following 3 chains:Factor X light chain; Factor X heavy chain; Activated factor Xa heavy chain
 Protein Family    Coagulation factor
 UniProt Gene Name    F10 [Similar Products]
 UniProt Entry Name    FA10_HUMAN
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 NCBI Summary for F10    This gene encodes the vitamin K-dependent coagulation factor X of the blood coagulation cascade. This factor undergoes multiple processing steps before its preproprotein is converted to a mature two-chain form by the excision of the tripeptide RKR. Two chains of the factor are held together by 1 or more disulfide bonds; the light chain contains 2 EGF-like domains, while the heavy chain contains the catalytic domain which is structurally homologous to those of the other hemostatic serine proteases. The mature factor is activated by the cleavage of the activation peptide by factor IXa (in the intrisic pathway), or by factor VIIa (in the extrinsic pathway). The activated factor then converts prothrombin to thrombin in the presence of factor Va, Ca+2, and phospholipid during blood clotting. Mutations of this gene result in factor X deficiency, a hemorrhagic condition of variable severity. [provided by RefSeq, Jul 2008]
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 UniProt Comments for F10    F10: Factor Xa is a vitamin K-dependent glycoprotein that converts prothrombin to thrombin in the presence of factor Va, calcium and phospholipid during blood clotting. Defects in F10 are the cause of factor X deficiency (FA10D). A hemorrhagic disease with variable presentation. Affected individuals can manifest prolonged nasal and mucosal hemorrhage, menorrhagia, hematuria, and occasionally hemarthrosis. Some patients do not have clinical bleeding diathesis. Belongs to the peptidase S1 family.

Protein type: Secreted, signal peptide; Secreted; Motility/polarity/chemotaxis; EC 3.4.21.6; Protease; Lipid-binding

Chromosomal Location of Human Ortholog: 13q34

Cellular Component: endoplasmic reticulum lumen; Golgi lumen; extracellular region; plasma membrane

Molecular Function: protein binding; serine-type endopeptidase activity; phospholipid binding; calcium ion binding

Biological Process: positive regulation of protein kinase B signaling cascade; blood coagulation, extrinsic pathway; cellular protein metabolic process; blood coagulation; proteolysis; post-translational protein modification; blood coagulation, intrinsic pathway; peptidyl-glutamic acid carboxylation; positive regulation of cell migration

Disease: Factor X Deficiency
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 Research Articles on F10    1. Prothrombin is proteolytically converted by factor Xa to the active protease thrombin in a reaction that is accelerated >3,000-fold by cofactor Va.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with F10 elisa kitPathways associated with F10 elisa kit
 Reference Product  PubMed Publications
 TFPI elisa kit  >2 publications with F10 and TFPI
 Products by Pathway  Pathway Diagram
 Blood Clotting Cascade Pathway antibodies  Blood Clotting Cascade Pathway Diagram
 Common Pathway antibodies  Common Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Extrinsic Pathway antibodies  Extrinsic Pathway Diagram
 Formation Of Fibrin Clot (Clotting Cascade) Pathway antibodies  Formation Of Fibrin Clot (Clotting Cascade) Pathway Diagram
 Gamma-carboxylation Of Protein Precursors Pathway antibodies  Gamma-carboxylation Of Protein Precursors Pathway Diagram
 Gamma-carboxylation, Transport, And Amino-terminal Cleavage Of Proteins Pathway antibodies  Gamma-carboxylation, Transport, And Amino-terminal Cleavage Of Proteins Pathway Diagram
 Hemostasis Pathway antibodies  Hemostasis Pathway Diagram
Diseases associated with F10 elisa kitOrgans/Tissues associated with F10 elisa kit
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >1032 publications with F10 and Neoplasms
 Lung Neoplasms Antibodies  >300 publications with F10 and Lung Neoplasms
 Drug Toxicity Antibodies  >77 publications with F10 and Drug Toxicity
 Nervous System Diseases Antibodies  >74 publications with F10 and Nervous System Diseases
 Necrosis Antibodies  >62 publications with F10 and Necrosis
 Liver Neoplasms Antibodies  >41 publications with F10 and Liver Neoplasms
 Brain Diseases Antibodies  >35 publications with F10 and Brain Diseases
 Inflammation Antibodies  >33 publications with F10 and Inflammation
 Liver Diseases Antibodies  >32 publications with F10 and Liver Diseases
 Liver Neoplasms, Experimental Antibodies  >31 publications with F10 and Liver Neoplasms, Experimental
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >436 publications with F10 and Blood
 Lung Antibodies  >414 publications with F10 and Lung
 Skin Antibodies  >132 publications with F10 and Skin
 Brain Antibodies  >97 publications with F10 and Brain
 Liver Antibodies  >88 publications with F10 and Liver
 Spleen Antibodies  >65 publications with F10 and Spleen
 Embryonic Tissue Antibodies  >52 publications with F10 and Embryonic Tissue
 Muscle Antibodies  >49 publications with F10 and Muscle
 Kidney Antibodies  >44 publications with F10 and Kidney
 Ovary Antibodies  >30 publications with F10 and Ovary
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