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Col II elisa kit :: Rat Collagen Type II ELISA Kit

Scan QR to view Datasheet Catalog #    MBS728101
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells-(Competitive)  /  $455 +1 FREE 8GB USB
48-Strip-Wells-(Sandwich)  /  $455 +1 FREE 8GB USB
96-Strip-Wells-(Competitive)  /  $660 +1 FREE 8GB USB
96-Strip-Wells-(Sandwich)  /  $660 +1 FREE 8GB USB
5x96-Strip-Wells-(Competitive)  /  $2,925 +3 FREE 8GB USB
5x96-Strip-Wells-(Sandwich)  /  $2,925 +3 FREE 8GB USB
10x96-Strip-Wells-(Competitive)  /  $5,490 +6 FREE 8GB USB
10x96-Strip-Wells-(Sandwich)  /  $5,490 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Collagen Type II (Col II), ELISA Kit

★Popular Item★
 Also Known As   

Rat Collagen Type II ELISA Kit

 Product Gene Name   

Col II elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    609508
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 Species Reactivity    Rat
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 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Assay Type    Competitive or Sandwich
 Detection Range    2.5-50ng/mL
 Sensitivity    0.1ng/ml
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of Col II elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for Col II purchase    MBS728101 is a ready-to-use microwell, strip plate Competitive or Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Collagen Type II (Col II) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 2.5-50ng/mL in biological research samples containing Col II, with an estimated sensitivity of 0.1ng/ml. The ELISA analytical biochemical technique of the MBS728101 kit is based on Col II antibody-Col II antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Col II antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Col II. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for Col II elisa kit

   Intended Uses: This CoL-2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat CoL-2. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: CoL-2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CoL-2 antibody and an CoL-2-HRP conjugate. The assay sample and buffer are incubated together with CoL-2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CoL-2 concentration since CoL-2 from samples and CoL-2-HRP conjugate compete for the anti-CoL-2 antibody binding site. Since the number of sites is limited, as more sites are occupied by CoL-2 from the sample, fewer sites are left to bind CoL-2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CoL-2 concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for Col II elisa kit    Cell Biology
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 Typical Testing Data/Standard Curve (for reference only) of Col II elisa kit    Col II elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS728101. Click to request current manual
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NCBI/Uniprot data below describe general gene information for Col II. It may not necessarily be applicable to this product.
 NCBI GI #    119578374
 NCBI GeneID    1280
 NCBI Accession #    EAW57970.1 [Other Products]
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 UniProt Secondary Accession #    Q12985; Q14009; Q14044; Q14045; Q14046; Q14047; Q14056; Q14058; Q16672; Q1JQ82; A6NGA0 [Other Products]
 UniProt Related Accession #    P02458 [Other Products]
 Molecular Weight    29,781 Da
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 NCBI Official Full Name    collagen, type II, alpha 1 (primary osteoarthritis, spondyloepiphyseal dysplasia, congenital), isoform CRA_e
 NCBI Official Synonym Full Names    collagen, type II, alpha 1
 NCBI Official Symbol    COL2A1 [Similar Products]
 NCBI Official Synonym Symbols   
AOM; ANFH; SEDC; STL1; COL11A3
[Similar Products]
 NCBI Protein Information    collagen alpha-1(II) chain; collagen alpha-1(II) chain; chondrocalcin; cartilage collagen; alpha-1 type II collagen; collagen II, alpha-1 polypeptide; arthroophthalmopathy, progressive (Stickler syndrome)
 UniProt Protein Name    Collagen alpha-1(II) chain
 UniProt Synonym Protein Names   
Alpha-1 type II collagenCleaved into the following 2 chains:Collagen alpha-1(II) chain; Chondrocalcin
 UniProt Gene Name    COL2A1 [Similar Products]
 UniProt Entry Name    CO2A1_HUMAN
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 NCBI Summary for Col II    This gene encodes the alpha-1 chain of type II collagen, a fibrillar collagen found in cartilage and the vitreous humor of the eye. Mutations in this gene are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer-Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type. In addition, defects in processing chondrocalcin, a calcium binding protein that is the C-propeptide of this collagen molecule, are also associated with chondrodysplasia. There are two transcripts identified for this gene. [provided by RefSeq, Jul 2008]
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 UniProt Comments for Col II    COL2A1: the alpha-1 chain of type II collagen, an extra-cellular matrix protein found in cartilage and the vitreous humor of the eye. It is essential for the normal embryonic development of the skeleton, for linear growth and for the ability of cartilage to resist compressive forces. Chondrocalcin is the calcium binding C-propeptide of this collagen molecule. Defects in this protein are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer-Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type. There are two transcripts identified for this gene. Belongs to the fibrillar collagen family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Extracellular matrix; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 12q13.11

Cellular Component: extracellular matrix; extracellular space; endoplasmic reticulum lumen; collagen type II; extracellular region; basement membrane

Molecular Function: identical protein binding; platelet-derived growth factor binding; metal ion binding; extracellular matrix structural constituent conferring tensile strength

Biological Process: heart morphogenesis; axon guidance; proteoglycan metabolic process; extracellular matrix organization and biogenesis; inner ear morphogenesis; collagen fibril organization; central nervous system development; palate development; notochord development; extracellular matrix disassembly; limb bud formation; collagen catabolic process; sensory perception of sound; visual perception; regulation of gene expression; cartilage development; tissue homeostasis; chondrocyte differentiation; skeletal development; cartilage condensation; endochondral ossification

Disease: Achondrogenesis, Type Ii; Kniest Dysplasia; Legg-calve-perthes Disease; Epiphyseal Dysplasia, Multiple, With Myopia And Conductive Deafness; Spondyloperipheral Dysplasia; Osteoarthritis With Mild Chondrodysplasia; Stickler Syndrome, Type I; Czech Dysplasia; Platyspondylic Lethal Skeletal Dysplasia, Torrance Type; Otospondylomegaepiphyseal Dysplasia; Spondyloepimetaphyseal Dysplasia, Strudwick Type; Avascular Necrosis Of Femoral Head, Primary; Spondyloepiphyseal Dysplasia Congenita; Stickler Syndrome, Type I, Nonsyndromic Ocular
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 Research Articles on Col II    1. study indicates that Set7/9 prevents the histone deacetylase activity of SirT1, potentiating euchromatin formation on the promoter site of COL2A1 and resulting in morphology-dependent COL2A1 gene transactivation.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with Col II elisa kitDiseases associated with Col II elisa kit
 Products by Pathway  Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Assembly Of Collagen Fibrils And Other Multimeric Structures Pathway antibodies  Assembly Of Collagen Fibrils And Other Multimeric Structures Pathway Diagram
 Collagen Biosynthesis And Modifying Enzymes Pathway antibodies  Collagen Biosynthesis And Modifying Enzymes Pathway Diagram
 Collagen Formation Pathway antibodies  Collagen Formation Pathway Diagram
 ECM-receptor Interaction Pathway antibodies  ECM-receptor Interaction Pathway Diagram
 ECM-receptor Interaction Pathway antibodies  ECM-receptor Interaction Pathway Diagram
 Endochondral Ossification Pathway antibodies  Endochondral Ossification Pathway Diagram
 Extracellular Matrix Organization Pathway antibodies  Extracellular Matrix Organization Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
 Disease Name  Pubmed Publications
 Osteoarthritis Antibodies  >224 publications with Col II and Osteoarthritis
 Osteochondrodysplasias Antibodies  >140 publications with Col II and Osteochondrodysplasias
 Retinal Detachment Antibodies  >52 publications with Col II and Retinal Detachment
 Myopia Antibodies  >43 publications with Col II and Myopia
 Cleft Palate Antibodies  >40 publications with Col II and Cleft Palate
 Craniofacial Abnormalities Antibodies  >37 publications with Col II and Craniofacial Abnormalities
 Necrosis Antibodies  >34 publications with Col II and Necrosis
 Kniest dysplasia Antibodies  >25 publications with Col II and Kniest dysplasia
 Hearing Loss, Sensorineural Antibodies  >23 publications with Col II and Hearing Loss, Sensorineural
 Inflammation Antibodies  >22 publications with Col II and Inflammation
Organs/Tissues associated with Col II elisa kit
 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >428 publications with Col II and Bone
 Connective Tissue Antibodies  >279 publications with Col II and Connective Tissue
 Embryonic Tissue Antibodies  >97 publications with Col II and Embryonic Tissue
 Eye Antibodies  >68 publications with Col II and Eye
 Muscle Antibodies  >23 publications with Col II and Muscle
 Brain Antibodies  >18 publications with Col II and Brain
 Kidney Antibodies  >15 publications with Col II and Kidney
 Ear Antibodies  >14 publications with Col II and Ear
 Skin Antibodies  >13 publications with Col II and Skin
 Heart Antibodies  >10 publications with Col II and Heart
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