Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource rightarrow ELISA Kit rightarrow C3b rightarrow Rat C3b  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Menu separator
separator
ELISA Kit ELISA Kit
CLIA Kit CLIA Kit
PCR Kit PCR Kit
Monoclonal Antibody Monoclonal Antibody
Polyclonal Antibody Polyclonal Antibody
Secondary Antibody Secondary Antibody
Antigen Antigen
Biochemical Biochemical
cDNA Clone cDNA Clone
siRNA siRNA
Peptide Peptide
Recombinant/Purified Protein Rec./Purified Protein

Custom ELISA Kit Custom ELISA Kit
Custom Protein Custom Protein
Custom Antibody Custom Antibody
Antibody Matched Pairs Antibody Matched Pairs
Antibody & Corresponding Blocking Peptide Pairs Antibody Peptide Pairs
Phospho-Specific Antibodies Phospho Antibodies
Products by Disease Products by Disease
Products by Pathway Products by Pathway
Products by Tissue Products by Tissue

arrow Advanced Search
arrow Submit Technical Q&A
arrow International Distributors
arrow Contact Us
separator
Our Best Sellers moreseparator
separator
 • Intestinal fatty acid binding protein (IFABP) ELISA Kit
 • Phospho Epidermal Growth Factor Receptor (P-EGFR) ELISA Kit
 • Wingless Type MMTV Integration Site Family, Member 1 (WNT1) ELISA Kit
 • GIF ELISA Kit
 • Keratinocyte Growth Factor (KGF) ELISA Kit
 • PDE3B Antibody
 • Ethinylestradiol HRP Conjugate
 • SOX9-SRY-box 9 Antibody
 • soluble fibrin monomer complex, SFMC ELISA Kit
 • Semaphorin 3C (SEMA3C) ELISA Kit
 • KR2_VZVD Antibody
 • GSK3b (Paired S9) (GSK3b (Paired S9)) Antibody
 • tumor necrosis factor, alpha-induced protein 6 (TNFAIP6) ELISA Kit
 • Myosin-7 (MYH7) Recombinant Protein
 downarrow more ...
separator
separator
DatasheetFull DatasheetPrinter Friendly DatasheetPrint This DatasheetAdd to Compare ListHave Questions? Ask UsRequest for a Quotation today

C3b elisa kit :: Rat Complement 3b (C3b) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS269481
Typical Testing Data/Standard Curve
Unit / Price
48-Strip-Wells  /  $275 +1 FREE 8GB USB
96-Strip-Wells  /  $435 +1 FREE 8GB USB
5x96-Strip-Wells  /  $1,755 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,150 +3 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Complement 3b (C3b), ELISA Kit

★Popular Item★
 Also Known As   

Rat Complement 3b (C3b) ELISA Kit

 Product Gene Name   

C3b elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
Table BarTOPTable Bar
  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    613779
Section Bar
 Species Reactivity    Rat
Section Bar
 Specificity    No cross-reaction with other factors.
Section Bar
 Samples    Rat serum, plasma or cell culture supernatant and organizations in the natural and recombinant C3b concentration.
 Assay Type    Sandwich
 Detection Range    100 ng/ml-1.56 ng/ml
 Sensitivity    0.5 ng/ml.
 Intra-assay Precision    <= 8%
 Inter-assay Precision    <= 12%
Section Bar
 Preparation and Storage    Store all reagents at 2-8 degree C.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of C3b elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for C3b purchase    MBS269481 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Complement 3b (C3b) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 100 ng/ml-1.56 ng/ml in biological research samples containing C3b, with an estimated sensitivity of 0.5 ng/ml. The ELISA analytical biochemical technique of the MBS269481 kit is based on C3b antibody-C3b antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect C3b antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, C3b. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Rat serum, plasma or cell culture supernatant and organizations in the natural and recombinant C3b concentration. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Table BarTOPTable Bar
 

Related Product Information for C3b elisa kit

   Principle of the Assay||This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Rat C3b monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
Section Bar
 Typical Testing Data/Standard Curve of C3b elisa kit    C3b elisa kit Typical Testing Data/Standard Curve image
Table BarTOPTable Bar
Sample Manual Insert of MBS269481. Click to request current manual
Table BarTOPTable Bar
NCBI/Uniprot data below describe general gene information for C3b. It may not necessarily be applicable to this product.
 NCBI GI #    115298678
 NCBI GeneID    718
 NCBI Accession #    NP_000055.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_000064.2 [Other Products]
Section Bar
 UniProt Secondary Accession #    A7E236 [Other Products]
 UniProt Related Accession #    P01024 [Other Products]
 Molecular Weight    187,148 Da
Table BarTOPTable Bar
 NCBI Official Full Name    complement C3
 NCBI Official Synonym Full Names    complement component 3
 NCBI Official Symbol    C3 [Similar Products]
 NCBI Official Synonym Symbols   
ASP; C3a; C3b; AHUS5; ARMD9; CPAMD1; HEL-S-62p
[Similar Products]
 NCBI Protein Information    complement C3; prepro-C3; C3a anaphylatoxin; complement component C3; complement component C3a; complement component C3b; acylation-stimulating protein cleavage product; epididymis secretory sperm binding protein Li 62p; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1
 UniProt Protein Name    Complement C3
 UniProt Synonym Protein Names   
C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1Cleaved into the following 11 chains:Complement C3 beta chain; Complement C3 alpha chain; C3a anaphylatoxin; Acylation stimulating protein; ASPAlternative name(s):C3adesArg
 UniProt Gene Name    C3 [Similar Products]
 UniProt Synonym Gene Names    CPAMD1; ASP [Similar Products]
 UniProt Entry Name    CO3_HUMAN
Section Bar
 NCBI Summary for C3b    Complement component C3 plays a central role in the activation of complement system. Its activation is required for both classical and alternative complement activation pathways. People with C3 deficiency are susceptible to bacterial infection. [provided by RefSeq, Feb 2009]
Section Bar
 UniProt Comments for C3b    C3: C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates. Defects in C3 are the cause of complement component 3 deficiency (C3D). A rare defect of the complement classical pathway. Patients develop recurrent, severe, pyogenic infections because of ineffective opsonization of pathogens. Some patients may also develop autoimmune disorders, such as arthralgia and vasculitic rashes, lupus-like syndrome and membranoproliferative glomerulonephritis. Genetic variation in C3 is associated with susceptibility to age-related macular degeneration type 9 (ARMD9). ARMD is a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid that lie beneath the retinal pigment epithelium and within an elastin- containing structure known as Bruch membrane. Defects in C3 are a cause of susceptibility to hemolytic uremic syndrome atypical type 5 (AHUS5). An atypical form of hemolytic uremic syndrome. It is a complex genetic disease characterized by microangiopathic hemolytic anemia, thrombocytopenia, renal failure and absence of episodes of enterocolitis and diarrhea. In contrast to typical hemolytic uremic syndrome, atypical forms have a poorer prognosis, with higher death rates and frequent progression to end-stage renal disease. Susceptibility to the development of atypical hemolytic uremic syndrome can be conferred by mutations in various components of or regulatory factors in the complement cascade system. Other genes may play a role in modifying the phenotype. Increased levels of C3 and its cleavage product ASP, are associated with obesity, diabetes and coronary heart disease. Short-term endurance training reduces baseline ASP levels and subsequently fat storage.

Protein type: Secreted, signal peptide; Inhibitor; Secreted

Chromosomal Location of Human Ortholog: 19p13.3-p13.2

Cellular Component: extracellular space; extracellular region; plasma membrane

Molecular Function: protein binding; endopeptidase inhibitor activity; C5L2 anaphylatoxin chemotactic receptor binding; receptor binding

Biological Process: regulation of immune response; complement activation, alternative pathway; signal transduction; fatty acid metabolic process; complement activation; G-protein coupled receptor protein signaling pathway; positive regulation of angiogenesis; positive regulation of activation of membrane attack complex; positive regulation of type IIa hypersensitivity; positive regulation of G-protein coupled receptor protein signaling pathway; regulation of complement activation; innate immune response; immune response; positive regulation of protein amino acid phosphorylation; inflammatory response; complement activation, classical pathway

Disease: Complement Component 3 Deficiency, Autosomal Recessive; Hemolytic Uremic Syndrome, Atypical, Susceptibility To, 5; Macular Degeneration, Age-related, 9
Table BarTOPTable Bar
 Research Articles on C3b    1. have identified Complement C3 as a candidate plasma biomarker for measuring disease state in neuroblastoma
Table BarTOPTable Bar
 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Table BarTOPTable Bar
Products associated with C3b elisa kitPathways associated with C3b elisa kit
 Reference Product  PubMed Publications
 CR1 elisa kit  >424 publications with C3b and CR1
 CR2 elisa kit  >293 publications with C3b and CR2
 CFH elisa kit  >206 publications with C3b and CFH
 CD46 elisa kit  >193 publications with C3b and CD46
 CFI elisa kit  >73 publications with C3b and CFI
 ITGAM elisa kit  >71 publications with C3b and ITGAM
 ITGAX elisa kit  >18 publications with C3b and ITGAX
 CFD elisa kit  >4 publications with C3b and CFD
 C3AR1 elisa kit  >3 publications with C3b and C3AR1
 Products by Pathway  Pathway Diagram
 Activation Of C3 And C5 Pathway antibodies  Activation Of C3 And C5 Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Alternative Complement Activation Pathway antibodies  Alternative Complement Activation Pathway Diagram
 Chagas Disease (American Trypanosomiasis) Pathway antibodies  Chagas Disease (American Trypanosomiasis) Pathway Diagram
 Chagas Disease (American Trypanosomiasis) Pathway antibodies  Chagas Disease (American Trypanosomiasis) Pathway Diagram
 Class A/1 (Rhodopsin-like Receptors) Pathway antibodies  Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
 Complement Activation, Classical Pathway antibodies  Complement Activation, Classical Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
Diseases associated with C3b elisa kitOrgans/Tissues associated with C3b elisa kit
 Disease Name  Pubmed Publications
 Kidney Diseases Antibodies  >3644 publications with C3b and Kidney Diseases
 Cardiovascular Diseases Antibodies  >2391 publications with C3b and Cardiovascular Diseases
 Inflammation Antibodies  >1915 publications with C3b and Inflammation
 Proteinuria Antibodies  >1270 publications with C3b and Proteinuria
 Lung Diseases Antibodies  >1011 publications with C3b and Lung Diseases
 Pain Antibodies  >989 publications with C3b and Pain
 Brain Diseases Antibodies  >941 publications with C3b and Brain Diseases
 Necrosis Antibodies  >927 publications with C3b and Necrosis
 Liver Diseases Antibodies  >923 publications with C3b and Liver Diseases
 Hemorrhage Antibodies  >771 publications with C3b and Hemorrhage
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >11810 publications with C3b and Blood
 Kidney Antibodies  >3490 publications with C3b and Kidney
 Bone Antibodies  >2910 publications with C3b and Bone
 Skin Antibodies  >2262 publications with C3b and Skin
 Brain Antibodies  >2096 publications with C3b and Brain
 Liver Antibodies  >1788 publications with C3b and Liver
 Muscle Antibodies  >1680 publications with C3b and Muscle
 Spinal Cord Antibodies  >1565 publications with C3b and Spinal Cord
 Nerve Antibodies  >1338 publications with C3b and Nerve
 Lung Antibodies  >1090 publications with C3b and Lung
Table BarTOPTable Bar
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions