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C3 elisa kit :: Rat Complement Component 3 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS760493
 Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $280 +1 FREE 8GB USB
96-Strip-Wells  /  $375 +1 FREE 8GB USB
5x96-Strip-Wells  /  $1,615 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,015 +3 FREE 8GB USB
15x96-Strip-Wells  /  $4,285 +5 FREE 8GB USB
25x96-Strip-Wells  /  $6,715 +8 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Complement Component 3 (C3), ELISA Kit

★Popular Item★
 Also Known As   

Rat Complement Component 3 ELISA Kit

 Product Synonym Names    C3
 Product Gene Name   

C3 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS760493 COA    COA PDF
 OMIM    120700
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 Species Reactivity    Rat
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 Specificity    This assay has high sensitivity and excellent specificity for detection of C3. No significant crossreactivity or interference between C3 and analogues was observed.
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 Samples    serum, plasma, tissue homogenates and other biological fluids
 Assay Type    Quantitative Sandwich
 Detection Range    3.125-200ng/ml
 Sensitivity    <1.875ng/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level C3 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
 Inter-assay Precision    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level C3 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
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 Preparation and Storage    Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of C3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for C3 purchase    MBS760493 is a ready-to-use microwell, strip plate Quantitative Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Complement Component 3 (C3) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 3.125-200ng/ml in biological research samples containing C3, with an estimated sensitivity of <1.875ng/ml. The ELISA analytical biochemical technique of the MBS760493 kit is based on C3 antibody-C3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect C3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, C3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as serum, plasma, tissue homogenates and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for C3 elisa kit

   Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- C3 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- C3 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the C3 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of C3 can be calculated.
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  Typical Testing Data/Standard Curve (for reference only) of C3 elisa kit    C3 elisa kit  Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS760493. Click to request current manual
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NCBI/Uniprot data below describe general gene information for C3. It may not necessarily be applicable to this product.
 NCBI GI #    152012784
 NCBI GeneID    718
 NCBI Accession #    AAI50180.1 [Other Products]
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 UniProt Secondary Accession #    A7E236 [Other Products]
 UniProt Related Accession #    P01024 [Other Products]
 Molecular Weight    187,148 Da
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 NCBI Official Full Name    Complement component 3
 NCBI Official Synonym Full Names    complement component 3
 NCBI Official Symbol    C3 [Similar Products]
 NCBI Official Synonym Symbols   
ASP; C3a; C3b; AHUS5; ARMD9; CPAMD1; HEL-S-62p
[Similar Products]
 NCBI Protein Information    complement C3
 UniProt Protein Name    Complement C3
 UniProt Synonym Protein Names   
C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1
 Protein Family    Complement C3
 UniProt Gene Name    C3 [Similar Products]
 UniProt Synonym Gene Names    CPAMD1; C3bc; ASP [Similar Products]
 UniProt Entry Name    CO3_HUMAN
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 NCBI Summary for C3    Complement component C3 plays a central role in the activation of complement system. Its activation is required for both classical and alternative complement activation pathways. The encoded preproprotein is proteolytically processed to generate alpha and beta subunits that form the mature protein, which is then further processed to generate numerous peptide products. The C3a peptide, also known as the C3a anaphylatoxin, modulates inflammation and possesses antimicrobial activity. Mutations in this gene are associated with atypical hemolytic uremic syndrome and age-related macular degeneration in human patients. [provided by RefSeq, Nov 2015]
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 UniProt Comments for C3    C3: C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates. Defects in C3 are the cause of complement component 3 deficiency (C3D). A rare defect of the complement classical pathway. Patients develop recurrent, severe, pyogenic infections because of ineffective opsonization of pathogens. Some patients may also develop autoimmune disorders, such as arthralgia and vasculitic rashes, lupus-like syndrome and membranoproliferative glomerulonephritis. Genetic variation in C3 is associated with susceptibility to age-related macular degeneration type 9 (ARMD9). ARMD is a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid that lie beneath the retinal pigment epithelium and within an elastin- containing structure known as Bruch membrane. Defects in C3 are a cause of susceptibility to hemolytic uremic syndrome atypical type 5 (AHUS5). An atypical form of hemolytic uremic syndrome. It is a complex genetic disease characterized by microangiopathic hemolytic anemia, thrombocytopenia, renal failure and absence of episodes of enterocolitis and diarrhea. In contrast to typical hemolytic uremic syndrome, atypical forms have a poorer prognosis, with higher death rates and frequent progression to end-stage renal disease. Susceptibility to the development of atypical hemolytic uremic syndrome can be conferred by mutations in various components of or regulatory factors in the complement cascade system. Other genes may play a role in modifying the phenotype. Increased levels of C3 and its cleavage product ASP, are associated with obesity, diabetes and coronary heart disease. Short-term endurance training reduces baseline ASP levels and subsequently fat storage.

Protein type: Inhibitor; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 19p13.3-p13.2

Cellular Component: extracellular region; extracellular space; plasma membrane

Molecular Function: C5L2 anaphylatoxin chemotactic receptor binding; cofactor binding; endopeptidase inhibitor activity; lipid binding; protein binding; receptor binding

Biological Process: blood coagulation; complement activation; complement activation, alternative pathway; complement activation, classical pathway; fatty acid metabolic process; G-protein coupled receptor protein signaling pathway; immune response; inflammatory response; innate immune response; positive regulation of activation of membrane attack complex; positive regulation of angiogenesis; positive regulation of developmental growth; positive regulation of G-protein coupled receptor protein signaling pathway; positive regulation of protein amino acid phosphorylation; positive regulation of type IIa hypersensitivity; regulation of complement activation; regulation of immune response; response to estradiol stimulus; response to glucocorticoid stimulus; response to magnesium ion; response to progesterone stimulus; signal transduction; tolerance induction

Disease: Complement Component 3 Deficiency, Autosomal Recessive; Hemolytic Uremic Syndrome, Atypical, Susceptibility To, 5; Macular Degeneration, Age-related, 9
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 Research Articles on C3    1. Plasmin cleavage of iC3b provides a complement regulatory pathway that is as efficient as FI/CR1 but does not require a cellular cofactor.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with C3 elisa kitPathways associated with C3 elisa kit
 Reference Product  PubMed Publications
 CR1 elisa kit  >438 publications with C3 and CR1
 CR2 elisa kit  >302 publications with C3 and CR2
 CFH elisa kit  >241 publications with C3 and CFH
 CD46 elisa kit  >213 publications with C3 and CD46
 CFI elisa kit  >89 publications with C3 and CFI
 ITGAM elisa kit  >76 publications with C3 and ITGAM
 CFHR1 elisa kit  >51 publications with C3 and CFHR1
 CFP elisa kit  >11 publications with C3 and CFP
 CFD elisa kit  >6 publications with C3 and CFD
 VSIG4 elisa kit  >1 publications with C3 and VSIG4
 Products by Pathway  Pathway Diagram
 Activation Of C3 And C5 Pathway antibodies  Activation Of C3 And C5 Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Alternative Complement Activation Pathway antibodies  Alternative Complement Activation Pathway Diagram
 Chagas Disease (American Trypanosomiasis) Pathway antibodies  Chagas Disease (American Trypanosomiasis) Pathway Diagram
 Chagas Disease (American Trypanosomiasis) Pathway antibodies  Chagas Disease (American Trypanosomiasis) Pathway Diagram
 Class A/1 (Rhodopsin-like Receptors) Pathway antibodies  Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
 Complement Activation, Classical Pathway antibodies  Complement Activation, Classical Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
Diseases associated with C3 elisa kitOrgans/Tissues associated with C3 elisa kit
 Disease Name  Pubmed Publications
 Kidney Diseases Antibodies  >3840 publications with C3 and Kidney Diseases
 Cardiovascular Diseases Antibodies  >2531 publications with C3 and Cardiovascular Diseases
 Inflammation Antibodies  >2105 publications with C3 and Inflammation
 Proteinuria Antibodies  >1347 publications with C3 and Proteinuria
 Lung Diseases Antibodies  >1046 publications with C3 and Lung Diseases
 Brain Diseases Antibodies  >1019 publications with C3 and Brain Diseases
 Necrosis Antibodies  >981 publications with C3 and Necrosis
 Liver Diseases Antibodies  >977 publications with C3 and Liver Diseases
 Hemorrhage Antibodies  >810 publications with C3 and Hemorrhage
 Anemia Antibodies  >794 publications with C3 and Anemia
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >12321 publications with C3 and Blood
 Kidney Antibodies  >3698 publications with C3 and Kidney
 Bone Antibodies  >3195 publications with C3 and Bone
 Skin Antibodies  >2343 publications with C3 and Skin
 Brain Antibodies  >2243 publications with C3 and Brain
 Liver Antibodies  >1891 publications with C3 and Liver
 Muscle Antibodies  >1770 publications with C3 and Muscle
 Spinal Cord Antibodies  >1686 publications with C3 and Spinal Cord
 Nerve Antibodies  >1411 publications with C3 and Nerve
 Lung Antibodies  >1139 publications with C3 and Lung
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