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C5a elisa kit :: Rat Complement Component 5a ELISA Kit

Scan QR to view Datasheet Catalog #    MBS760397
 Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $300 +1 FREE 8GB USB
96-Strip-Wells  /  $415 +1 FREE 8GB USB
5x96-Strip-Wells  /  $1,825 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,425 +4 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Complement Component 5a (C5a), ELISA Kit

★Popular Item★
 Also Known As   

Rat Complement Component 5a ELISA Kit

 Product Synonym Names    C5a
 Product Gene Name   

C5a elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS760397 COA    COA PDF
 OMIM    113995
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 Species Reactivity    Rat
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 Specificity    This assay has high sensitivity and excellent specificity for detection of C5a. No significant cross-reactivity or interference between C5a and analogues was observed.
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 Samples    serum, plasma, tissue homogenates and other biological fluids
 Assay Type    Quantitative Sandwich
 Detection Range    62.5-4000pg/ml
 Sensitivity    <37.5pg/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level C5a were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
 Inter-assay Precision    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level C5a were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<10%
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 Preparation and Storage    Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of C5a elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for C5a purchase    MBS760397 is a ready-to-use microwell, strip plate Quantitative Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Complement Component 5a (C5a) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 62.5-4000pg/ml in biological research samples containing C5a, with an estimated sensitivity of <37.5pg/ml. The ELISA analytical biochemical technique of the MBS760397 kit is based on C5a antibody-C5a antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect C5a antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, C5a. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as serum, plasma, tissue homogenates and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for C5a elisa kit

   Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- C5a antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-C5a antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the C5a amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of C5a can be calculated.
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  Typical Testing Data/Standard Curve (for reference only) of C5a elisa kit    C5a elisa kit  Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS760397. Click to request current manual
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NCBI/Uniprot data below describe general gene information for C5a. It may not necessarily be applicable to this product.
 NCBI GI #    29169287
 NCBI GeneID    728
 NCBI Accession #    AAO65969.1 [Other Products]
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 UniProt Related Accession #    P21730 [Other Products]
 Molecular Weight    39,336 Da
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 NCBI Official Full Name    complement component 5a receptor 1
 NCBI Official Synonym Full Names    complement component 5a receptor 1
 NCBI Official Symbol    C5AR1 [Similar Products]
 NCBI Official Synonym Symbols   
C5A; C5AR; C5R1; CD88
[Similar Products]
 NCBI Protein Information    C5a anaphylatoxin chemotactic receptor 1
 UniProt Protein Name    C5a anaphylatoxin chemotactic receptor 1
 UniProt Synonym Protein Names   
C5a anaphylatoxin chemotactic receptor; C5a-R; C5aR; CD_antigen: CD88
 UniProt Gene Name    C5AR1 [Similar Products]
 UniProt Synonym Gene Names    C5AR; C5R1; C5a-R; C5aR [Similar Products]
 UniProt Entry Name    C5AR1_HUMAN
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 UniProt Comments for C5a    C5aR: a family 1 G-protein coupled receptor that stimulates the GTPase activity of Gi2. Receptor for the chemotactic and inflammatory complement factor C5a. Stimulates chemotaxis, granule enzyme release and superoxide anion production. Phosphorylated in response to C5a or after stimulation of cells with phorbol esters. Expressed on monocytes, granulocytes, dendritic cells, astrocytes and microglia.

Protein type: Receptor, GPCR; GPCR, family 1; Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 19q13.3-q13.4

Cellular Component: apical part of cell; basolateral plasma membrane; cell surface; cytoplasmic membrane-bound vesicle; cytosol; integral to plasma membrane; plasma membrane

Molecular Function: C5a anaphylatoxin receptor activity; complement component C5a binding; complement component C5a receptor activity

Biological Process: activation of MAPK activity; apoptosis; cell proliferation in hindbrain; cellular defense response; chemotaxis; defense response to Gram-positive bacterium; elevation of cytosolic calcium ion concentration; immune response; inflammatory response; mRNA transcription from RNA polymerase II promoter; negative regulation of neuron apoptosis; neutrophil chemotaxis; organ regeneration; phospholipase C activation; positive regulation of angiogenesis; positive regulation of epithelial cell proliferation; response to lipopolysaccharide; response to peptidoglycan; sensory perception of chemical stimulus; signal transduction
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 Research Articles on C5a    1. 96.7% of the metastatic RCCs had C5aR expression, vs. 50.5% of the non-metastatic ones. C5a triggered ERK- and PI3Kdependent invasion of the C5aR-expressing renal carcinoma cells. The C5a-C5aR axis plays a crucial role in renal carcinoma cell invasion.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with C5a elisa kitPathways associated with C5a elisa kit
 Reference Product  PubMed Publications
 IL8 elisa kit  >4 publications with C5a and IL8
 C3AR1 elisa kit  >2 publications with C5a and C3AR1
 CXCL12 elisa kit  >2 publications with C5a and CXCL12
 Products by Pathway  Pathway Diagram
 Class A/1 (Rhodopsin-like Receptors) Pathway antibodies  Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 G Alpha (i) Signalling Events Pathway antibodies  G Alpha (i) Signalling Events Pathway Diagram
 GPCR Downstream Signaling Pathway antibodies  GPCR Downstream Signaling Pathway Diagram
 GPCR Ligand Binding Pathway antibodies  GPCR Ligand Binding Pathway Diagram
 Neuroactive Ligand-receptor Interaction Pathway antibodies  Neuroactive Ligand-receptor Interaction Pathway Diagram
 Neuroactive Ligand-receptor Interaction Pathway antibodies  Neuroactive Ligand-receptor Interaction Pathway Diagram
 Peptide GPCRs Pathway antibodies  Peptide GPCRs Pathway Diagram
Diseases associated with C5a elisa kitOrgans/Tissues associated with C5a elisa kit
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >60 publications with C5a and Inflammation
 Immune System Diseases Antibodies  >27 publications with C5a and Immune System Diseases
 Cardiovascular Diseases Antibodies  >15 publications with C5a and Cardiovascular Diseases
 Necrosis Antibodies  >13 publications with C5a and Necrosis
 Kidney Diseases Antibodies  >10 publications with C5a and Kidney Diseases
 Nervous System Diseases Antibodies  >10 publications with C5a and Nervous System Diseases
 Lung Diseases Antibodies  >8 publications with C5a and Lung Diseases
 Atherosclerosis Antibodies  >7 publications with C5a and Atherosclerosis
 Fibrosis Antibodies  >5 publications with C5a and Fibrosis
 Lung Injury Antibodies  >4 publications with C5a and Lung Injury
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >71 publications with C5a and Blood
 Bone Marrow Antibodies  >8 publications with C5a and Bone Marrow
 Brain Antibodies  >6 publications with C5a and Brain
 Intestine Antibodies  >5 publications with C5a and Intestine
 Placenta Antibodies  >3 publications with C5a and Placenta
 Uterus Antibodies  >1 publications with C5a and Uterus
 Prostate Antibodies  >1 publications with C5a and Prostate
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