Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource rightarrow ELISA Kit rightarrow CDK4 rightarrow Rat CDK4  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Menu separator
separator
ELISA Kit ELISA Kit
CLIA Kit CLIA Kit
PCR Kit PCR Kit
Monoclonal Antibody Monoclonal Antibody
Polyclonal Antibody Polyclonal Antibody
Secondary Antibody Secondary Antibody
Antigen Antigen
Biochemical Biochemical
cDNA Clone cDNA Clone
siRNA siRNA
Peptide Peptide
Recombinant/Purified Protein Rec./Purified Protein

Custom ELISA Kit Custom ELISA Kit
Custom Protein Custom Protein
Custom Antibody Custom Antibody
Antibody Matched Pairs Antibody Matched Pairs
Antibody & Corresponding Blocking Peptide Pairs Antibody Peptide Pairs
Phospho-Specific Antibodies Phospho Antibodies
Products by Disease Products by Disease
Products by Pathway Products by Pathway
Products by Tissue Products by Tissue

arrow Advanced Search
arrow Submit Technical Q&A
arrow International Distributors
arrow Contact Us
separator
Our Best Sellers moreseparator
separator
 • Renal Dipeptidase - DPEP1 Antibody
 • F-box only protein 32 (FBXO32) ELISA Kit
 • Placental Lactogen (PL) ELISA Kit
 • C1 Esterase Inhibitor (SERPING1) Antibody
 • Klotho Antibody
 • Skim Milk Agar Culture Media
 • Abnormal prothrombin (APT) - Des-gamma-carboxy prothrombin (... ELISA Kit
 • Peptidase D (PEPD) ELISA Kit
 • KLF2 Antibody
 • Interleukin-10 (IL-10) Antibody
 • G protein-coupled receptor 124 (GPR124) ELISA Kit
 • ZC3HC1 Antibody
 • TNPO3 Antibody
 • CASP7 ELISA Kit
 downarrow more ...
separator
separator
DatasheetFull DatasheetPrinter Friendly DatasheetPrint This DatasheetAdd to Compare ListHave Questions? Ask UsRequest for a Quotation today

CDK4 elisa kit :: Rat Cyclin Dependent Kinase 4 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2533543
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $410 +1 FREE 8GB USB
96-Strip-Wells  /  $490 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,040 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,590 +4 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Cyclin Dependent Kinase 4 (CDK4), ELISA Kit

★Popular Item★
 Also Known As   

Rat CDK4 (Cyclin Dependent Kinase 4) ELISA Kit

 Product Gene Name   

CDK4 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
Table BarTOPTable Bar
  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    123829
 3D Structure    ModBase 3D Structure for P11802
Section Bar
 Species Reactivity    Rat
Section Bar
 Specificity    This kit recognizes natural and recombinantRatCDK4. No significant cross-reactivity or interference between RatCDK4 and analogues was observed.
Section Bar
 Samples    Serum, Plasma, Biological Fluids
 Assay Type    Sandwich
 Detection Range    78.125-5000pg/mL
 Sensitivity    Min: 46.875pg/mL ; Max: 5000pg/mL
Section Bar
 Preparation and Storage    Store at 4 degree C.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of CDK4 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for CDK4 purchase    MBS2533543 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Cyclin Dependent Kinase 4 (CDK4) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 78.125-5000pg/mL in biological research samples containing CDK4, with an estimated sensitivity of Min: 46.875pg/mL ; Max: 5000pg/mL. The ELISA analytical biochemical technique of the MBS2533543 kit is based on CDK4 antibody-CDK4 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CDK4 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CDK4. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma, Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Table BarTOPTable Bar
 

Related Product Information for CDK4 elisa kit

   Intended Uses: This ELISA kit applies to the invitro quantitative determination of RatCDK4concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to CDK4. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for CDK4and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain CDK4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of CDK4.You can calculate the concentration of CDK4in the samples by comparing the OD of the samples to the standard curve.
Section Bar
 Typical Testing Data/Standard Curve (for reference only) of CDK4 elisa kit    CDK4 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Table BarTOPTable Bar
Sample Manual Insert of MBS2533543. Click to request current manual
Table BarTOPTable Bar
NCBI/Uniprot data below describe general gene information for CDK4. It may not necessarily be applicable to this product.
 NCBI GI #    4502735
 NCBI GeneID    1019
 NCBI Accession #    NP_000066.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_000075.3 [Other Products]
Section Bar
 UniProt Primary Accession #    P11802 [Other Products]
 UniProt Secondary Accession #    O00576; Q6FG61; B2R9A0; B4DNF9 [Other Products]
 UniProt Related Accession #    P11802 [Other Products]
 Molecular Weight    20,725 Da
Table BarTOPTable Bar
 NCBI Official Full Name    cyclin-dependent kinase 4
 NCBI Official Synonym Full Names    cyclin dependent kinase 4
 NCBI Official Symbol    CDK4 [Similar Products]
 NCBI Official Synonym Symbols   
CMM3; PSK-J3
[Similar Products]
 NCBI Protein Information    cyclin-dependent kinase 4
 UniProt Protein Name    Cyclin-dependent kinase 4
 UniProt Synonym Protein Names   
Cell division protein kinase 4; PSK-J3
 Protein Family    Cyclin-dependent kinase
 UniProt Gene Name    CDK4 [Similar Products]
 UniProt Entry Name    CDK4_HUMAN
Section Bar
 NCBI Summary for CDK4    The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is highly similar to the gene products of S. cerevisiae cdc28 and S. pombe cdc2. It is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression. The activity of this kinase is restricted to the G1-S phase, which is controlled by the regulatory subunits D-type cyclins and CDK inhibitor p16(INK4a). This kinase was shown to be responsible for the phosphorylation of retinoblastoma gene product (Rb). Mutations in this gene as well as in its related proteins including D-type cyclins, p16(INK4a) and Rb were all found to be associated with tumorigenesis of a variety of cancers. Multiple polyadenylation sites of this gene have been reported. [provided by RefSeq, Jul 2008]
Section Bar
 UniProt Comments for CDK4    CDK4: a protein kinase of the CDK family that is important for cell cycle G1 phase progression. Its activity is restricted to the G1-S phase. Controlled by the regulatory subunits D-type cyclins and CDK inhibitor p16(INK4a). Phosphorylates the retinoblastoma gene product (Rb). Point mutations found in somatic and familial melanoma. Amplified in sarcomas, glioma and lymphoma. Amplified, methylated or deleted in head and neck squamous cell carcinoma. Overexpression drives epithelial tumors in mice. Disruption makes mice resistant to cancer. Inhibitor: PD332991.

Protein type: Protein kinase, Ser/Thr (non-receptor); Cell cycle regulation; EC 2.7.11.22; Protein kinase, CMGC; Kinase, protein; CMGC group; CDK family; CDK/CDK4 subfamily; CDK4 subfamily

Chromosomal Location of Human Ortholog: 12q14

Cellular Component: chromatin; cyclin-dependent protein kinase holoenzyme complex; cytosol; nuclear membrane; nucleolus; nucleoplasm; nucleus; perinuclear region of cytoplasm; tight junction; transcription factor complex

Molecular Function: ATP binding; cyclin binding; cyclin-dependent protein kinase activity; cyclin-dependent protein kinase regulator activity; protein binding; protein complex binding

Biological Process: cell division; circadian rhythm; establishment and/or maintenance of chromatin architecture; G1/S transition of mitotic cell cycle; lens development in camera-type eye; mitotic cell cycle; organ regeneration; positive regulation of apoptosis; positive regulation of cell proliferation; positive regulation of cell size; positive regulation of fibroblast proliferation; positive regulation of translation; protein amino acid phosphorylation; regulation of gene expression; regulation of protein kinase activity; response to drug; response to hyperoxia; response to lead ion; response to testosterone stimulus; response to toxin; signal transduction

Disease: Melanoma, Cutaneous Malignant, Susceptibility To, 3
Table BarTOPTable Bar
 Research Articles on CDK4    1. both miR-613 mimics and inhibitors could decrease and increase CDK4 protein levels in non-small cell lung cancer-derived cells, respectively.
Table BarTOPTable Bar
 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Table BarTOPTable Bar
Products associated with CDK4 elisa kitPathways associated with CDK4 elisa kit
 Reference Product  PubMed Publications
 CDKN2A elisa kit  >1066 publications with CDK4 and CDKN2A
 CDKN1A elisa kit  >803 publications with CDK4 and CDKN1A
 CDKN1B elisa kit  >696 publications with CDK4 and CDKN1B
 CCND1 elisa kit  >255 publications with CDK4 and CCND1
 CCND3 elisa kit  >157 publications with CDK4 and CCND3
 CCND2 elisa kit  >153 publications with CDK4 and CCND2
 CDKN2B elisa kit  >124 publications with CDK4 and CDKN2B
 CDKN2C elisa kit  >103 publications with CDK4 and CDKN2C
 RB1 elisa kit  >95 publications with CDK4 and RB1
 CDC37 elisa kit  >32 publications with CDK4 and CDC37
 Products by Pathway  Pathway Diagram
 AGE-RAGE Signaling Pathway In Diabetic Complications antibodies  AGE-RAGE Signaling Pathway In Diabetic Complications Diagram
 AGE-RAGE Signaling Pathway In Diabetic Complications antibodies  AGE-RAGE Signaling Pathway In Diabetic Complications Diagram
 ATF-2 Transcription Factor Network Pathway antibodies  ATF-2 Transcription Factor Network Pathway Diagram
 B Cell Receptor Signaling Pathway antibodies  B Cell Receptor Signaling Pathway Diagram
 Bladder Cancer Pathway antibodies  Bladder Cancer Pathway Diagram
 Bladder Cancer Pathway antibodies  Bladder Cancer Pathway Diagram
 Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway antibodies  Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle, Mitotic Pathway antibodies  Cell Cycle, Mitotic Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
Diseases associated with CDK4 elisa kitOrgans/Tissues associated with CDK4 elisa kit
 Disease Name  Pubmed Publications
 Carcinoma Antibodies  >866 publications with CDK4 and Carcinoma
 Breast Neoplasms Antibodies  >348 publications with CDK4 and Breast Neoplasms
 Adenocarcinoma Antibodies  >345 publications with CDK4 and Adenocarcinoma
 Cell Transformation, Neoplastic Antibodies  >246 publications with CDK4 and Cell Transformation, Neoplastic
 Skin Neoplasms Antibodies  >220 publications with CDK4 and Skin Neoplasms
 Neoplasms, Experimental Antibodies  >202 publications with CDK4 and Neoplasms, Experimental
 Lung Neoplasms Antibodies  >188 publications with CDK4 and Lung Neoplasms
 Liver Diseases Antibodies  >186 publications with CDK4 and Liver Diseases
 Liver Neoplasms Antibodies  >174 publications with CDK4 and Liver Neoplasms
 Carcinoma, Hepatocellular Antibodies  >166 publications with CDK4 and Carcinoma, Hepatocellular
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >451 publications with CDK4 and Blood
 Lung Antibodies  >332 publications with CDK4 and Lung
 Liver Antibodies  >307 publications with CDK4 and Liver
 Muscle Antibodies  >297 publications with CDK4 and Muscle
 Skin Antibodies  >295 publications with CDK4 and Skin
 Brain Antibodies  >293 publications with CDK4 and Brain
 Vascular Antibodies  >241 publications with CDK4 and Vascular
 Bone Antibodies  >195 publications with CDK4 and Bone
 Prostate Antibodies  >167 publications with CDK4 and Prostate
 Kidney Antibodies  >119 publications with CDK4 and Kidney
Table BarTOPTable Bar
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions