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COX8B elisa kit :: Rat Cytochrome c oxidase subunit 8B, mitochondrial (COX8B) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS7236047
Typical Testing Data/Standard Curve
Unit / Price
48-Strip-Wells  /  $440 +1 FREE 8GB USB
96-Strip-Wells  /  $640 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
 
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 Product Name   

Cytochrome c oxidase subunit 8B, mitochondrial (COX8B), ELISA Kit

★Popular Item★
 Also Known As   

Rat Cytochrome c oxidase subunit 8B, mitochondrial (COX8B) ELISA Kit

 Product Gene Name   

COX8B elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 Species Reactivity    Rat
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 Specificity    This assay has high sensitivity and excellent specificity for detection of COX8B. No significant cross-reactivity or interference between COX8B and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between COX8B and all the analogues, therefore, cross reaction may still exist in some cases.
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 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Assay Type    Competitive
 Detection Range    0.5-10ng/mL
 Sensitivity    0.1ng/ml
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of COX8B elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for COX8B purchase    MBS7236047 is a ready-to-use microwell, strip plate Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Cytochrome c oxidase subunit 8B, mitochondrial (COX8B) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.5-10ng/mL in biological research samples containing COX8B, with an estimated sensitivity of 0.1ng/ml. The ELISA analytical biochemical technique of the MBS7236047 kit is based on COX8B antibody-COX8B antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect COX8B antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, COX8B. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for COX8B elisa kit

   Intended Uses: This COX8B ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat COX8B. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay||COX8B ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-COX8B antibody and an COX8B-HRP conjugate. The assay sample and buffer are incubated together with COX8B-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the COX8B concentration since COX8B from samples and COX8B-HRP conjugate compete for the anti-COX8B antibody binding site. Since the number of sites is limited, as more sites are occupied by COX8B from the sample, fewer sites are left to bind COX8B-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The COX8B concentration in each sample is interpolated from this standard curve.
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 Typical Testing Data/Standard Curve of COX8B elisa kit    COX8B elisa kit Typical Testing Data/Standard Curve image
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Sample Manual Insert of MBS7236047. Click to request current manual
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NCBI/Uniprot data below describe general gene information for COX8B. It may not necessarily be applicable to this product.
 NCBI GI #    158517805
 NCBI GeneID    25250
 NCBI Accession #    NP_036918.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_012786.3 [Other Products]
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 UniProt Related Accession #    P16221 [Other Products]
 Molecular Weight    7,549 Da
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 NCBI Official Full Name    cytochrome c oxidase subunit 8B, mitochondrial
 NCBI Official Synonym Full Names    cytochrome c oxidase, subunit VIIIb
 NCBI Official Symbol    Cox8b [Similar Products]
 NCBI Official Synonym Symbols   
CCO8A; Cox8h; COXVIII-H
[Similar Products]
 NCBI Protein Information    cytochrome c oxidase subunit 8B, mitochondrial; cytochrome c oxidase subunit 8H; cytochrome c oxidase subunit 8-1; cytochrome c oxidase, subunit 8B; cytochrome c oxidase polypeptide VIII-heart; cytochrome c oxidase, subunit VIIIb, muscle; Cytochrom c oxidase subunit VIII-H (heart/muscle); Cytochrome c oxidase subunit VIII-H (heart/muscle)
 UniProt Protein Name    Cytochrome c oxidase subunit 8B, mitochondrial
 UniProt Synonym Protein Names   
Cytochrome c oxidase polypeptide VIII-heart; Cytochrome c oxidase subunit 8-1; Cytochrome c oxidase subunit 8H
 UniProt Gene Name    Cox8b [Similar Products]
 UniProt Synonym Gene Names    Cox8h [Similar Products]
 UniProt Entry Name    COX8B_RAT
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 UniProt Comments for COX8B    COX8B: This protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. Belongs to the cytochrome c oxidase VIII family.

Protein type: Membrane protein, integral; Oxidoreductase; Mitochondrial

Cellular Component: mitochondrial inner membrane; integral to membrane

Molecular Function: cytochrome-c oxidase activity

Biological Process: response to drug; mitochondrial electron transport, cytochrome c to oxygen
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with COX8B elisa kitPathways associated with COX8B elisa kit
 Reference Product  PubMed Publications
 Cox6a2 elisa kit  >2 publications with COX8B and Cox6a2
 Uqcrq elisa kit  >1 publications with COX8B and Uqcrq
 Products by Pathway  Pathway Diagram
 Alzheimer's Disease Pathway antibodies  Alzheimer's Disease Pathway Diagram
 Alzheimer's Disease Pathway antibodies  Alzheimer's Disease Pathway Diagram
 Cardiac Muscle Contraction Pathway antibodies  Cardiac Muscle Contraction Pathway Diagram
 Cardiac Muscle Contraction Pathway antibodies  Cardiac Muscle Contraction Pathway Diagram
 Cytochrome C Oxidase Pathway antibodies  Cytochrome C Oxidase Pathway Diagram
 Cytochrome C Oxidase Pathway antibodies  Cytochrome C Oxidase Pathway Diagram
 Huntington's Disease Pathway antibodies  Huntington's Disease Pathway Diagram
 Huntington's Disease Pathway antibodies  Huntington's Disease Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram
 Non-alcoholic Fatty Liver Disease (NAFLD) Pathway antibodies  Non-alcoholic Fatty Liver Disease (NAFLD) Pathway Diagram
Diseases associated with COX8B elisa kitOrgans/Tissues associated with COX8B elisa kit
 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >1 publications with COX8B and Nervous System Diseases
 Immune System Diseases Antibodies  >1 publications with COX8B and Immune System Diseases
 Fibrosis Antibodies  >1 publications with COX8B and Fibrosis
 Liver Cirrhosis Antibodies  >1 publications with COX8B and Liver Cirrhosis
 Liver Diseases Antibodies  >1 publications with COX8B and Liver Diseases
 Organ/Tissue Name  Pubmed Publications
 Adipose Tissue Antibodies  >6 publications with COX8B and Adipose Tissue
 Brain Antibodies  >1 publications with COX8B and Brain
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