Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource rightarrow ELISA Kit rightarrow DEFb2 rightarrow Rat DEFb2  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Menu separator
separator
ELISA Kit ELISA Kit
CLIA Kit CLIA Kit
PCR Kit PCR Kit
Monoclonal Antibody Monoclonal Antibody
Polyclonal Antibody Polyclonal Antibody
Secondary Antibody Secondary Antibody
Antigen Antigen
Biochemical Biochemical
cDNA Clone cDNA Clone
siRNA siRNA
Peptide Peptide
Recombinant/Purified Protein Rec./Purified Protein

Custom ELISA Kit Custom ELISA Kit
Custom Protein Custom Protein
Custom Antibody Custom Antibody
Antibody Matched Pairs Antibody Matched Pairs
Antibody & Corresponding Blocking Peptide Pairs Antibody Peptide Pairs
Phospho-Specific Antibodies Phospho Antibodies
Products by Disease Products by Disease
Products by Pathway Products by Pathway
Products by Tissue Products by Tissue

arrow Advanced Search
arrow Submit Technical Q&A
arrow International Distributors
arrow Contact Us
separator
Our Best Sellers moreseparator
separator
 • Abl interactor 2 (Abi2) Recombinant Protein
 • squamous cell carcinoma antigen recognized by T cells 3 (SA... Antibody
 • 26S protease regulatory subunit 7 (PSMC2) Recombinant Protein
 • CRP Native Protein
 • Nibrin (NBN) Recombinant Protein
 • Creatine Kinase, Muscle (CKM) ELISA Kit
 • ACVR1 Antibody
 • AFP Antibody
 • Rse Triiodothyronine (rT3 ) ELISA Kit
 • Desmosine ELISA Kit
 • Alcohol dehydrogenase 1A (ADH1A) Recombinant Protein
 • Fatty Acid Binding Protein-7 (FABP7) Recombinant Protein
 • corin, serine peptidase ELISA Kit
 • SBF2 Antibody
 downarrow more ...
separator
separator
DatasheetFull DatasheetPrinter Friendly DatasheetPrint This DatasheetAdd to Compare ListHave Questions? Ask UsRequest for a Quotation today

DEFb2 elisa kit :: Rat Defensin Beta 2 (DEFb2) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS456564
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $395 +1 FREE 8GB USB
96-Strip-Wells  /  $525 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,380 +2 FREE 8GB USB
10x96-Strip-Wells  /  $4,620 +5 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Defensin Beta 2 (DEFb2), ELISA Kit

 Also Known As   

Rat Defensin Beta 2 (DEFb2) ELISA Kit

 Product Synonym Names    B-DF2; DEFB2; BD2; DEF-B2; HBD2; DEFB102; HBD-2; SAP1; DEFB4A; DEFB4; Skin-Antimicrobial Peptide 1
 Product Gene Name   

DEFb2 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
Table BarTOPTable Bar
  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 3D Structure    ModBase 3D Structure for Q32ZI5
 Species Reactivity    Rat
Section Bar
 Specificity    This assay has high sensitivity and excellent specificity for detection of DEFb2. No significant cross-reactivity or interference between DEFb2 and analogues was observed.
Section Bar
 Samples    Rat serum, plasma, tissue homogenates and other biological fluids
 Assay Type    Quantitative Sandwich
 Detection Range    78.12-5000pg/mL
 Sensitivity    <22.61pg/mL
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level DEFb2 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
 Inter-assay Precision    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level DEFb2 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Section Bar
 Preparation and Storage    For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of DEFb2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for DEFb2 purchase    MBS456564 is a ready-to-use microwell, strip plate Quantitative Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Defensin Beta 2 (DEFb2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 78.12-5000pg/mL in biological research samples containing DEFb2, with an estimated sensitivity of <22.61pg/mL. The ELISA analytical biochemical technique of the MBS456564 kit is based on DEFb2 antibody-DEFb2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect DEFb2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, DEFb2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Rat serum, plasma, tissue homogenates and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Table BarTOPTable Bar
 

Related Product Information for DEFb2 elisa kit

   Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of DEFb2 in rat serum, plasma, tissue homogenates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to DEFb2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to DEFb2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain DEFb2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of DEFb2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Section Bar
 Typical Testing Data/Standard Curve (for reference only) of DEFb2 elisa kit    DEFb2 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Table BarTOPTable Bar
Sample Manual Insert of MBS456564. Click to request current manual
Table BarTOPTable Bar
NCBI/Uniprot data below describe general gene information for DEFb2. It may not necessarily be applicable to this product.
 NCBI GI #    82830447
 NCBI GeneID    641631
 NCBI Accession #    NP_001032596.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001037507.1 [Other Products]
Section Bar
 UniProt Primary Accession #    Q32ZI5 [Other Products]
 Molecular Weight    8,119 Da
Table BarTOPTable Bar
 NCBI Official Full Name    beta-defensin 2
 NCBI Official Synonym Full Names    defensin beta 2
 NCBI Official Symbol    Defb2 [Similar Products]
 NCBI Protein Information    beta-defensin 2
 UniProt Protein Name    Beta-defensin 2
 UniProt Synonym Protein Names   
Beta-defensin 2
 UniProt Gene Name    Defb2 [Similar Products]
Section Bar
 Research Articles on DEFb2    1. The lowering of BD-2 expression mechanically ventilated rats after bacterial challenge might be one of the contributory factors in causing ventilator-associated pneumonia.
Table BarTOPTable Bar
 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Table BarTOPTable Bar
Diseases associated with DEFb2 elisa kit
 Disease Name  Pubmed Publications
 Disease Models, Animal Antibodies  >5 publications with DEFb2 and Disease Models, Animal
 Necrosis Antibodies  >4 publications with DEFb2 and Necrosis
 Intestinal Diseases Antibodies  >3 publications with DEFb2 and Intestinal Diseases
 Inflammation Antibodies  >3 publications with DEFb2 and Inflammation
 Fibrosis Antibodies  >3 publications with DEFb2 and Fibrosis
 Edema Antibodies  >1 publications with DEFb2 and Edema
 Infertility, Male Antibodies  >1 publications with DEFb2 and Infertility, Male
Table BarTOPTable Bar
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions