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DKK1 elisa kit :: Rat Dickkopf Related Protein 1 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS732071
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $440 +1 FREE 8GB USB
96-Strip-Wells  /  $640 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Dickkopf Related Protein 1 (DKK1), ELISA Kit

★Popular Item★
 Also Known As   

Rat Dickkopf Related Protein 1 ELISA Kit

 Product Gene Name   

DKK1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    605189
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 Species Reactivity    Rat
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 Specificity    This assay has high sensitivity and excellent specificity for detection of DKK1. No significant cross-reactivity or interference between DKK1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between DKK1 and all the analogues, therefore, cross reaction may still exist in some cases.
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 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Assay Type    Competitive
 Detection Range    50-1000pg/mL
 Sensitivity    1.0pg/ml
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of DKK1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for DKK1 purchase    MBS732071 is a ready-to-use microwell, strip plate Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Dickkopf Related Protein 1 (DKK1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 50-1000pg/mL in biological research samples containing DKK1, with an estimated sensitivity of 1.0pg/ml. The ELISA analytical biochemical technique of the MBS732071 kit is based on DKK1 antibody-DKK1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect DKK1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, DKK1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for DKK1 elisa kit

   Intended Uses: This DKK1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat DKK1.

Principle of the Assay: DKK1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-DKK1 antibody and an DKK1-HRP conjugate. The assay sample and buffer are incubated together with DKK1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the DKK1 concentration since DKK1 from samples and DKK1-HRP conjugate compete for the anti-DKK1 antibody binding site. Since the number of sites is limited, as more sites are occupied by DKK1 from the sample, fewer sites are left to bind DKK1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The DKK1 concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for DKK1 elisa kit    Immunology
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 Typical Testing Data/Standard Curve (for reference only) of DKK1 elisa kit    DKK1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS732071. Click to request current manual
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NCBI/Uniprot data below describe general gene information for DKK1. It may not necessarily be applicable to this product.
 NCBI GI #    7110719
 NCBI GeneID    22943
 NCBI Accession #    NP_036374.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_012242.2 [Other Products]
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 UniProt Secondary Accession #    B2RC19 [Other Products]
 UniProt Related Accession #    O94907 [Other Products]
 Molecular Weight    28,672 Da
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 NCBI Official Full Name    dickkopf-related protein 1
 NCBI Official Synonym Full Names    dickkopf WNT signaling pathway inhibitor 1
 NCBI Official Symbol    DKK1 [Similar Products]
 NCBI Official Synonym Symbols   
SK; DKK-1
[Similar Products]
 NCBI Protein Information    dickkopf-related protein 1; hDkk-1; dickkopf-1 like; dickkopf 1 homolog; dickkopf-like protein 1; dickkopf related protein-1
 UniProt Protein Name    Dickkopf-related protein 1
 UniProt Synonym Protein Names   
SK
 Protein Family    Dickkopf-related protein
 UniProt Gene Name    DKK1 [Similar Products]
 UniProt Synonym Gene Names    UNQ492/PRO1008; Dickkopf-1; Dkk-1; hDkk-1 [Similar Products]
 UniProt Entry Name    DKK1_HUMAN
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 NCBI Summary for DKK1    This gene encodes a protein that is a member of the dickkopf family. It is a secreted protein with two cysteine rich regions and is involved in embryonic development through its inhibition of the WNT signaling pathway. Elevated levels of DKK1 in bone marrow plasma and peripheral blood is associated with the presence of osteolytic bone lesions in patients with multiple myeloma. [provided by RefSeq, Jul 2008]
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 UniProt Comments for DKK1    DKK1: Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero- posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease. Interacts with LRP6. Interacts (via the C-termianl Cys- rich domain) with LRP5 (via beta-propeller regions 3 and 4); the interaction, enhanced by MESD and or KREMEN, antagonizes Wnt- mediated signaling. Placenta. Belongs to the dickkopf family.

Protein type: Inhibitor; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 10q11.2

Cellular Component: extracellular space; early endosome membrane; plasma membrane; extracellular region

Molecular Function: signal transducer activity; protein binding; receptor antagonist activity; growth factor activity; low-density lipoprotein receptor binding

Biological Process: cellular morphogenesis during differentiation; negative regulation of skeletal muscle development; response to retinoic acid; negative regulation of Wnt receptor signaling pathway; regulation of receptor internalization; negative regulation of peptidyl-serine phosphorylation; negative regulation of transcription from RNA polymerase II promoter; negative regulation of BMP signaling pathway; mesoderm formation; hair follicle development; negative regulation of mesodermal cell fate specification; endoderm formation; forebrain development; regulation of endodermal cell fate specification; negative regulation of protein complex assembly; embryonic limb morphogenesis
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 Research Articles on DKK1    1. Dickkopf-1 is oncogenic and involved in invasive growth in non small cell lung cancer.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with DKK1 elisa kitPathways associated with DKK1 elisa kit
 Reference Product  PubMed Publications
 CTNNB1 elisa kit  >473 publications with DKK1 and CTNNB1
 WNT3A elisa kit  >126 publications with DKK1 and WNT3A
 LRP5 elisa kit  >115 publications with DKK1 and LRP5
 LRP6 elisa kit  >101 publications with DKK1 and LRP6
 WNT1 elisa kit  >38 publications with DKK1 and WNT1
 KREMEN1 elisa kit  >17 publications with DKK1 and KREMEN1
 KREMEN2 elisa kit  >15 publications with DKK1 and KREMEN2
 RSPO1 elisa kit  >10 publications with DKK1 and RSPO1
 FZD1 elisa kit  >10 publications with DKK1 and FZD1
 FZD8 elisa kit  >4 publications with DKK1 and FZD8
 Products by Pathway  Pathway Diagram
 Cardiac Progenitor Differentiation Pathway antibodies  Cardiac Progenitor Differentiation Pathway Diagram
 Direct P53 Effectors Pathway antibodies  Direct P53 Effectors Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Presenilin Action In Notch And Wnt Signaling Pathway antibodies  Presenilin Action In Notch And Wnt Signaling Pathway Diagram
 RNF Mutants Show Enhanced WNT Signaling And Proliferation Pathway antibodies  RNF Mutants Show Enhanced WNT Signaling And Proliferation Pathway Diagram
 Regulation Of Wnt-mediated Beta Catenin Signaling And Target Gene Transcription Pathway antibodies  Regulation Of Wnt-mediated Beta Catenin Signaling And Target Gene Transcription Pathway Diagram
 Signal Transduction Pathway antibodies  Signal Transduction Pathway Diagram
 Signaling By WNT In Cancer Pathway antibodies  Signaling By WNT In Cancer Pathway Diagram
 Signaling By Wnt Pathway antibodies  Signaling By Wnt Pathway Diagram
 TCF Dependent Signaling In Response To WNT Pathway antibodies  TCF Dependent Signaling In Response To WNT Pathway Diagram
Diseases associated with DKK1 elisa kitOrgans/Tissues associated with DKK1 elisa kit
 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >86 publications with DKK1 and Cardiovascular Diseases
 Hematologic Diseases Antibodies  >69 publications with DKK1 and Hematologic Diseases
 Nervous System Diseases Antibodies  >40 publications with DKK1 and Nervous System Diseases
 Inflammation Antibodies  >37 publications with DKK1 and Inflammation
 Brain Diseases Antibodies  >36 publications with DKK1 and Brain Diseases
 Breast Neoplasms Antibodies  >34 publications with DKK1 and Breast Neoplasms
 Necrosis Antibodies  >33 publications with DKK1 and Necrosis
 Carcinoma, Hepatocellular Antibodies  >32 publications with DKK1 and Carcinoma, Hepatocellular
 Neoplasm Metastasis Antibodies  >30 publications with DKK1 and Neoplasm Metastasis
 Fractures, Bone Antibodies  >28 publications with DKK1 and Fractures, Bone
 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >460 publications with DKK1 and Bone
 Embryonic Tissue Antibodies  >100 publications with DKK1 and Embryonic Tissue
 Bone Marrow Antibodies  >98 publications with DKK1 and Bone Marrow
 Brain Antibodies  >76 publications with DKK1 and Brain
 Connective Tissue Antibodies  >75 publications with DKK1 and Connective Tissue
 Vascular Antibodies  >55 publications with DKK1 and Vascular
 Liver Antibodies  >49 publications with DKK1 and Liver
 Skin Antibodies  >46 publications with DKK1 and Skin
 Muscle Antibodies  >46 publications with DKK1 and Muscle
 Lung Antibodies  >39 publications with DKK1 and Lung
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