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BH2 elisa kit :: Rat Dihydrobiopterin ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2533583
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $410 +1 FREE 8GB USB
96-Strip-Wells  /  $490 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,040 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,590 +4 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Dihydrobiopterin (BH2), ELISA Kit

★Popular Item★
 Also Known As   

Rat BH2 (Dihydrobiopterin) ELISA Kit

 Product Gene Name   

BH2 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS2533583 COA    COA PDF
 Species Reactivity    Rat
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 Specificity    This kit recognizes natural and recombinantRatBH2. No significant cross-reactivity or interference between RatBH2 and analogues was observed.
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 Samples    Serum, plasma and other biological fluids
 Assay Type    Quantitative Competitive
 Detection Range    31.25-2000pg/mL
 Sensitivity    18.75pg/mL
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 Preparation and Storage    Store at 4 degree C.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of BH2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for BH2 purchase    MBS2533583 is a ready-to-use microwell, strip plate Quantitative Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Dihydrobiopterin (BH2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 31.25-2000pg/mL in biological research samples containing BH2, with an estimated sensitivity of 18.75pg/mL. The ELISA analytical biochemical technique of the MBS2533583 kit is based on BH2 antibody-BH2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect BH2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, BH2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for BH2 elisa kit

   Intended Uses: This ELISA kit applies to the invitro quantitative determination of RatBH2concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with BH2. During the reaction,BH2 in the sample or standard competes with a fixed amount ofBH2on the solid phase supporterfor sites on the Biotinylated Detection Ab specific toBH2. Excess conjugate and unbound sample or standard are washed from the plate, andAvidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Thena TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration ofBH2 in the samples is then determined by comparing the OD of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of BH2 elisa kit    BH2 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2533583. Click to request current manual
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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