View our list of available Coronavirus (COVID-19, SARS-CoV-2, 2019-nCOV) antibodies, recombinant proteins, ELISA Kits... Options Full Datasheet Printer Friendly Datasheet Prepare for Printing Full Datasheet Printer Friendly Datasheet Prepare for Printing Have Questions? Ask Us! Request A Quote Today! Add To Compare List DPA elisa kit :: Rat Dopamine ELISA Kit Catalog # MBS725908 Unit / Price 48-Strip-Wells / $495 +1 FREE 8GB USB 96-Strip-Wells / $700 +1 FREE 8GB USB 5x96-Strip-Wells / $3,105 +3 FREE 8GB USB 10x96-Strip-Wells / $5,865 +7 FREE 8GB USB Product Name Product Info Accession #s Product Desc Diseases/Tissues/Pathways Applications References Product Name Dopamine (DPA), ELISA Kit Popular Item Full Product Name Rat Dopamine ELISA Kit Product Gene Name DPA elisa kit[Similar Products] Research Use Only For Research Use Only. Not for use in diagnostic procedures. TOP Sample Manual Insert Download Sample PDF Manual View Sample PDF Manual MBS725908 COA COA PDF Species Reactivity Rat Specificity This assay has high sensitivity and excellent specificity for detection of DA. No significant cross-reactivity or interference between DA and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between DA and all the analogues, therefore, cross reaction may still exist in some cases. Samples Serum, plasma, cell culture supernatants, body fluid and tissue homogenate Assay Type Quantitative Competitive Sensitivity 1.0 ng/mL Preparation and Storage Store all reagents at 2-8 degree C. Sample Preparation We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team ISO Certification Manufactured in an ISO 13485:2016 Certified Laboratory. Product Note Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details. Other Notes Small volumes of DPA elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply. Searchable Terms for DPA purchase MBS725908 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Dopamine (DPA) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing DPA. The ELISA analytical biochemical technique of the MBS725908 kit is based on DPA antibody-DPA antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect DPA antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, DPA. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%). Usually associated with neurodegenerative diseases, dopamine DPA is synthesized in species as diverse as human, mouse to plants from dopamine beta hydroxylase. Given this wide pattern, a specific assay for each species, such as rat or human DPA, is a critical hurdle. MyBiosource, world leader in protein and peptide reagents, offers you the best dopamine ELISA on the market today. Forget about old school RIA dopamine assay kit results. Our DPA ELISA kits are fast and economical solid phase plate designs for your valuable cell lysates, plasma or urine sample types. Factor in our sensitive ELISA primary antibody and secondary antibodies to detect proteins in a few cells and the value skyrockets. Dopamine ELISA kits include product review sheets and suggestions for applications. DPA ELISA kit can be ordered as 1 plate or strip formats for research convenience. Find all our fine ELISA assay kit products in our online catalog. TOP Related Product Information for DPA elisa kit Intended Uses: This DA ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat DA. This ELISA kit for research use only, not for therapeutic or test applications!Principle of the Assay: DA ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-DA antibody and an DA-HRP conjugate. The assay sample and buffer are incubated together with DA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the DA concentration since DA from samples and DA-HRP conjugate compete for the anti-DA antibody binding site. Since the number of sites is limited, as more sites are occupied by DA from the sample, fewer sites are left to bind DA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The DA concentration in each sample is interpolated from this standard curve. Product Categories/Family for DPA elisa kit Neurobiology Typical Testing Data/Standard Curve (for reference only) of DPA elisa kit TOP Sample Manual Insert of MBS725908. Protein Family Dopamine receptor Precautions All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines. Disclaimer While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information. TOP