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ENA-78 elisa kit :: Rat ENA-78 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2512820
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $410 +1 FREE 8GB USB
96-Strip-Wells  /  $490 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,040 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,590 +4 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

ENA-78, ELISA Kit

★Popular Item★
 Also Known As   

Rat ENA-78 (Epithelial Neutrophil Activating Peptide 78) ELISA Kit

 Product Gene Name   

ENA-78 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    600324
 3D Structure    ModBase 3D Structure for P42830
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 Species Reactivity    Rat
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Rat ENA-78. No significant cross-reactivity or interference between Rat ENA-78 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Rat ENA-78 and all the analogues, therefore, cross reaction may still exist.
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 Samples    Serum, Plasma, Biological Fluids
 Assay Type    Sandwich
 Detection Range    15.625-1000pg/mL
 Sensitivity    Min: 9.375pg/mL; Max: 1000pg/mL
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 Preparation and Storage    Store at 4 degree C.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of ENA-78 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for ENA-78 purchase    MBS2512820 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the ENA-78, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 15.625-1000pg/mL in biological research samples containing ENA-78, with an estimated sensitivity of Min: 9.375pg/mL; Max: 1000pg/mL. The ELISA analytical biochemical technique of the MBS2512820 kit is based on ENA-78 antibody-ENA-78 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ENA-78 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ENA-78. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma, Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for ENA-78 elisa kit

   Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of ENA-78 in Rat serum, plasma and other biological fluids
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat ENA-78. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Rat ENA-78 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat ENA-78, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Rat ENA-78. You can calculate the concentration of Rat ENA-78 in the samples by comparing the OD of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of ENA-78 elisa kit    ENA-78 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2512820. Click to request current manual
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NCBI/Uniprot data below describe general gene information for ENA-78. It may not necessarily be applicable to this product.
 NCBI GI #    471243
 NCBI GeneID    6374
 NCBI Accession #    CAA55355.1 [Other Products]
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 UniProt Primary Accession #    P42830 [Other Products]
 UniProt Secondary Accession #    Q96QE1 [Other Products]
 UniProt Related Accession #    P42830 [Other Products]
 Molecular Weight    11,972 Da
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 NCBI Official Full Name    ENA-78
 NCBI Official Synonym Full Names    chemokine (C-X-C motif) ligand 5
 NCBI Official Symbol    CXCL5 [Similar Products]
 NCBI Official Synonym Symbols   
SCYB5; ENA-78
[Similar Products]
 NCBI Protein Information    C-X-C motif chemokine 5; epithelial-derived neutrophil-activating protein 78; neutrophil-activating peptide ENA-78; small inducible cytokine subfamily B (Cys-X-Cys), member 5 (epithelial-derived neutrophil-activating peptide 78)
 UniProt Protein Name    C-X-C motif chemokine 5
 UniProt Synonym Protein Names   
ENA-78(1-78); Epithelial-derived neutrophil-activating protein 78; Neutrophil-activating peptide ENA-78; Small-inducible cytokine B5Cleaved into the following 2 chains:ENA-78(8-78); ENA-78(9-78)
 UniProt Gene Name    CXCL5 [Similar Products]
 UniProt Synonym Gene Names    ENA78; SCYB5 [Similar Products]
 UniProt Entry Name    CXCL5_HUMAN
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 NCBI Summary for ENA-78    This gene encodes a protein that is a member of the CXC subfamily of chemokines. Chemokines, which recruit and activate leukocytes, are classified by function (inflammatory or homeostatic) or by structure. This protein is proposed to bind the G-protein coupled receptor chemokine (C-X-C motif) receptor 2 to recruit neutrophils, to promote angiogenesis and to remodel connective tissues. This protein is thought to play a role in cancer cell proliferation, migration, and invasion. [provided by RefSeq, May 2013]
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 UniProt Comments for ENA-78    CXCL5: Involved in neutrophil activation. In vitro, ENA-78(8- 78) and ENA-78(9-78) show a threefold higher chemotactic activity for neutrophil granulocytes. Belongs to the intercrine alpha (chemokine CxC) family.

Protein type: Secreted; Secreted, signal peptide; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 4q13.3

Cellular Component: extracellular space; extracellular region

Molecular Function: chemokine activity; CXCR chemokine receptor binding

Biological Process: G-protein coupled receptor protein signaling pathway; cell-cell signaling; positive regulation of cell proliferation; neutrophil mediated immunity; positive regulation of leukocyte chemotaxis; response to lipopolysaccharide; immune response; chemotaxis; inflammatory response; signal transduction
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 Research Articles on ENA-78    1. the expression levels of CXCL5 proteins were decreased in dermal blood vessels of early stage diffuse cutaneous systemic sclerosis
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with ENA-78 elisa kitPathways associated with ENA-78 elisa kit
 Reference Product  PubMed Publications
 CXCR2 elisa kit  >87 publications with ENA-78 and CXCR2
 CCL5 elisa kit  >73 publications with ENA-78 and CCL5
 CCL20 elisa kit  >41 publications with ENA-78 and CCL20
 CXCR1 elisa kit  >26 publications with ENA-78 and CXCR1
 CXCL11 elisa kit  >21 publications with ENA-78 and CXCL11
 CXCL16 elisa kit  >11 publications with ENA-78 and CXCL16
 CCR3 elisa kit  >8 publications with ENA-78 and CCR3
 CCR1 elisa kit  >8 publications with ENA-78 and CCR1
 CCL19 elisa kit  >8 publications with ENA-78 and CCL19
 CCR5 elisa kit  >8 publications with ENA-78 and CCR5
 Products by Pathway  Pathway Diagram
 Chemokine Receptors Bind Chemokines Pathway antibodies  Chemokine Receptors Bind Chemokines Pathway Diagram
 Chemokine Signaling Pathway antibodies  Chemokine Signaling Pathway Diagram
 Chemokine Signaling Pathway antibodies  Chemokine Signaling Pathway Diagram
 Class A/1 (Rhodopsin-like Receptors) Pathway antibodies  Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway antibodies  Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 G Alpha (i) Signalling Events Pathway antibodies  G Alpha (i) Signalling Events Pathway Diagram
 GPCR Downstream Signaling Pathway antibodies  GPCR Downstream Signaling Pathway Diagram
Diseases associated with ENA-78 elisa kitOrgans/Tissues associated with ENA-78 elisa kit
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >239 publications with ENA-78 and Inflammation
 Necrosis Antibodies  >101 publications with ENA-78 and Necrosis
 Respiratory Tract Diseases Antibodies  >100 publications with ENA-78 and Respiratory Tract Diseases
 Lung Diseases Antibodies  >89 publications with ENA-78 and Lung Diseases
 Disease Models, Animal Antibodies  >65 publications with ENA-78 and Disease Models, Animal
 Carcinoma Antibodies  >60 publications with ENA-78 and Carcinoma
 Cardiovascular Diseases Antibodies  >45 publications with ENA-78 and Cardiovascular Diseases
 Fibrosis Antibodies  >28 publications with ENA-78 and Fibrosis
 Lung Neoplasms Antibodies  >27 publications with ENA-78 and Lung Neoplasms
 Pneumonia Antibodies  >25 publications with ENA-78 and Pneumonia
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >305 publications with ENA-78 and Blood
 Lung Antibodies  >131 publications with ENA-78 and Lung
 Vascular Antibodies  >98 publications with ENA-78 and Vascular
 Intestine Antibodies  >34 publications with ENA-78 and Intestine
 Skin Antibodies  >29 publications with ENA-78 and Skin
 Kidney Antibodies  >17 publications with ENA-78 and Kidney
 Brain Antibodies  >17 publications with ENA-78 and Brain
 Connective Tissue Antibodies  >16 publications with ENA-78 and Connective Tissue
 Embryonic Tissue Antibodies  >14 publications with ENA-78 and Embryonic Tissue
 Uterus Antibodies  >13 publications with ENA-78 and Uterus
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