View our list of available Coronavirus (COVID-19, SARS-CoV-2, 2019-nCOV) antibodies, recombinant proteins, ELISA Kits... Options Full Datasheet Printer Friendly Datasheet Prepare for Printing Full Datasheet Printer Friendly Datasheet Prepare for Printing Have Questions? Ask Us! Request A Quote Today! Add To Compare List ENK elisa kit :: Rat Enkephalin ELISA Kit Catalog # MBS726498 Unit / Price 48-Strip-Wells / $495 +1 FREE 8GB USB 96-Strip-Wells / $700 +1 FREE 8GB USB 5x96-Strip-Wells / $3,105 +3 FREE 8GB USB 10x96-Strip-Wells / $5,865 +7 FREE 8GB USB Product Name Product Info Accession #s Product Desc Diseases/Tissues/Pathways Applications References Product Name Enkephalin, ELISA Kit Popular Item Full Product Name Rat Enkephalin ELISA Kit Product Gene Name ENK elisa kit[Similar Products] Research Use Only For Research Use Only. Not for use in diagnostic procedures. TOP Sample Manual Insert Download Sample PDF Manual View Sample PDF Manual Species Reactivity Rat Specificity This assay has high sensitivity and excellent specificity for detection of ENK. No significant cross-reactivity or interference between ENK and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ENK and all the analogues, therefore, cross reaction may still exist in some cases. Samples Serum, plasma, cell culture supernatants, body fluid and tissue homogenate Assay Type Quantitative Competitive Sensitivity 1.0 pg/mL Preparation and Storage Store all reagents at 2-8 degree C. Sample Preparation We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team ISO Certification Manufactured in an ISO 13485:2016 Certified Laboratory. Product Note Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details. Other Notes Small volumes of ENK elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply. Searchable Terms for ENK purchase MBS726498 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Enkephalin, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ENK. The ELISA analytical biochemical technique of the MBS726498 kit is based on ENK antibody-ENK antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ENK antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ENK. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%). TOP Related Product Information for ENK elisa kit Intended Uses: This ENK ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat ENK. This ELISA kit for research use only, not for therapeutic or test applications!Principle of the Assay: ENK ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-ENK antibody and an ENK-HRP conjugate. The assay sample and buffer are incubated together with ENK-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ENK concentration since ENK from samples and ENK-HRP conjugate compete for the anti-ENK antibody binding site. Since the number of sites is limited, as more sites are occupied by ENK from the sample, fewer sites are left to bind ENK-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ENK concentration in each sample is interpolated from this standard curve. Product Categories/Family for ENK elisa kit Neurobiology Typical Testing Data/Standard Curve (for reference only) of ENK elisa kit TOP Sample Manual Insert of MBS726498. NCBI/Uniprot data below describe general gene information for ENK. It may not necessarily be applicable to this product. NCBI GI # 204026 NCBI GeneID 29237 NCBI Accession # AAA41115.1 [Other Products] UniProt Secondary Accession # Q53X05[Other Products] UniProt Related Accession # P04094[Other Products] Molecular Weight 30,932 Da TOP NCBI Official Full Name enkephalin NCBI Official Synonym Full Names proenkephalin NCBI Official Symbol Penk [Similar Products] NCBI Official Synonym Symbols Enk; Penk1; Penk2; Penk-rs [Similar Products] NCBI Protein Information proenkephalin-A; proenkephalin 1; proenkephalin 2; preproenkephalin 2; proenkephalin related sequence UniProt Protein Name Proenkephalin-A UniProt Synonym Protein Names Opioid growth factor; OGF UniProt Gene Name Penk [Similar Products] UniProt Synonym Gene Names Penk-rs; Penk1; OGF [Similar Products] UniProt Entry Name PENK_RAT TOP NCBI Summary for ENK neuropeptide hormone; plays a role in the responses to stress and pain perception [RGD, Feb 2006] TOP UniProt Comments for ENK PENK: Met- and Leu-enkephalins compete with and mimic the effects of opiate drugs. They play a role in a number of physiologic functions, including pain perception and responses to stress. PENK(114-133) and PENK(237-258) increase glutamate release in the striatum. PENK(114-133) decreases GABA concentration in the striatum. Belongs to the opioid neuropeptide precursor family.Protein type: Secreted; Secreted, signal peptide; Hormone Research Articles on ENK 1. results suggest that a DeltaFosB-mediated downregulation of enkephalin in the nucleus accumbens might underlie the susceptibility to chronic stress TOP Precautions All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines. Disclaimer While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information. TOP Products associated with ENK elisa kit Reference Product PubMed Publications Pdyn elisa kit >43 publications with ENK and Pdyn Pomc elisa kit >31 publications with ENK and Pomc Jun elisa kit >21 publications with ENK and Jun Fos elisa kit >20 publications with ENK and Fos Oprm1 elisa kit >4 publications with ENK and Oprm1 Oprd1 elisa kit >3 publications with ENK and Oprd1 Fhl2 elisa kit >2 publications with ENK and Fhl2 Oprk1 elisa kit >2 publications with ENK and Oprk1 Pathways associated with ENK elisa kit Products by Pathway Pathway Diagram Class A/1 (Rhodopsin-like Receptors) Pathway antibodies Class A/1 (Rhodopsin-like Receptors) Pathway Diagram G Alpha (i) Signalling Events Pathway antibodies G Alpha (i) Signalling Events Pathway Diagram GPCR Downstream Signaling Pathway antibodies GPCR Downstream Signaling Pathway Diagram GPCR Ligand Binding Pathway antibodies GPCR Ligand Binding Pathway Diagram Peptide Ligand-binding Receptors Pathway antibodies Peptide Ligand-binding Receptors Pathway Diagram Signal Transduction Pathway antibodies Signal Transduction Pathway Diagram Signaling By GPCR Pathway antibodies Signaling By GPCR Pathway Diagram Diseases associated with ENK elisa kit Disease Name Pubmed Publications Nervous System Diseases Antibodies >33 publications with ENK and Nervous System Diseases Opioid-Related Disorders Antibodies >30 publications with ENK and Opioid-Related Disorders Brain Diseases Antibodies >21 publications with ENK and Brain Diseases Anxiety Disorders Antibodies >21 publications with ENK and Anxiety Disorders Pain Antibodies >14 publications with ENK and Pain Disease Models, Animal Antibodies >14 publications with ENK and Disease Models, Animal Movement Disorders Antibodies >11 publications with ENK and Movement Disorders Seizures Antibodies >7 publications with ENK and Seizures Cognition Disorders Antibodies >7 publications with ENK and Cognition Disorders Cocaine-Related Disorders Antibodies >6 publications with ENK and Cocaine-Related Disorders Organs/Tissues associated with ENK elisa kit Organ/Tissue Name Pubmed Publications Brain Antibodies >113 publications with ENK and Brain Heart Antibodies >8 publications with ENK and Heart Pituitary Gland Antibodies >6 publications with ENK and Pituitary Gland Prostate Antibodies >3 publications with ENK and Prostate Eye Antibodies >3 publications with ENK and Eye Embryonic Tissue Antibodies >2 publications with ENK and Embryonic Tissue TOP © 2006-24 MyBioSource.com. 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