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HE-4 elisa kit :: Rat Epididymis Protein 4 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS755820
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $440 +1 FREE 8GB USB
96-Strip-Wells  /  $640 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Epididymis Protein 4 (HE-4), ELISA Kit

★Popular Item★
 Also Known As   

Rat Epididymis Protein 4 ELISA Kit

 Product Gene Name   

HE-4 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 Species Reactivity    Rat
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 Specificity    This assay has high sensitivity and excellent specificity for detection of RE4. No significant cross-reactivity or interference between RE4 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between RE4 and all the analogues, therefore, cross reaction may still exist in some cases.
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 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Assay Type    Competitive
 Sensitivity    1.0 pmol/ml.
 Intended Uses    This RE4 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat RE4. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of HE-4 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for HE-4 purchase    MBS755820 is a ready-to-use microwell, strip plate Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Epididymis Protein 4 (HE-4) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing HE-4, with an estimated sensitivity of 1.0 pmol/ml. The ELISA analytical biochemical technique of the MBS755820 kit is based on HE-4 antibody-HE-4 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect HE-4 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, HE-4. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for HE-4 elisa kit

   Principle of the assay: RE4 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-RE4 antibody and an RE4-HRP conjugate. The assay sample and buffer are incubated together with RE4-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the RE4 concentration since RE4 from samples and RE4-HRP conjugate compete for the anti-RE4 antibody binding site. Since the number of sites is limited, as more sites are occupied by RE4 from the sample, fewer sites are left to bind RE4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The RE4 concentration in each sample is interpolated from this standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of HE-4 elisa kit    HE-4 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS755820. Click to request current manual
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NCBI/Uniprot data below describe general gene information for HE-4. It may not necessarily be applicable to this product.
 NCBI GI #    56699495
 NCBI GeneID    10406
 NCBI Accession #    NP_006094.3 [Other Products]
 NCBI GenBank Nucleotide #    NM_006103.3 [Other Products]
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 UniProt Secondary Accession #    Q6IB27; Q8WXV9; Q8WXW0; Q8WXW1; Q8WXW2; Q96KJ1; A2A2A5; A2A2A6; A6PVD5 [Other Products]
 UniProt Related Accession #    Q14508 [Other Products]
 Molecular Weight    11,043 Da
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 NCBI Official Full Name    WAP four-disulfide core domain protein 2
 NCBI Official Synonym Full Names    WAP four-disulfide core domain 2
 NCBI Official Symbol    WFDC2 [Similar Products]
 NCBI Official Synonym Symbols   
HE4; WAP5; EDDM4; dJ461P17.6
[Similar Products]
 NCBI Protein Information    WAP four-disulfide core domain protein 2; epididymal protein 4; epididymal secretory protein E4; putative protease inhibitor WAP5; WAP domain containing protein HE4-V4; major epididymis-specific protein E4; epididymis-specific, whey-acidic protein type, four-disulfide core
 UniProt Protein Name    WAP four-disulfide core domain protein 2
 UniProt Synonym Protein Names   
Epididymal secretory protein E4; Major epididymis-specific protein E4; Putative protease inhibitor WAP5
 UniProt Gene Name    WFDC2 [Similar Products]
 UniProt Synonym Gene Names    HE4; WAP5 [Similar Products]
 UniProt Entry Name    WFDC2_HUMAN
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 NCBI Summary for HE-4    This gene encodes a protein that is a member of the WFDC domain family. The WFDC domain, or WAP Signature motif, contains eight cysteines forming four disulfide bonds at the core of the protein, and functions as a protease inhibitor in many family members. This gene is expressed in pulmonary epithelial cells, and was also found to be expressed in some ovarian cancers. The encoded protein is a small secretory protein, which may be involved in sperm maturation. [provided by RefSeq, Jul 2008]
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 UniProt Comments for HE-4    WFDC2: a protein that is a member of the WFDC domain family. The WFDC domain, or WAP Signature motif, contains eight cysteines forming four disulfide bonds at the core of the protein, and functions as a protease inhibitor in many family members. This gene is expressed in pulmonary epithelial cells, and was also found to be expressed in some ovarian cancers. The encoded protein is a small secretory protein, which may be involved in sperm maturation. [provided by RefSeq, Jul 2008]

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 20q13.12

Cellular Component: extracellular space

Molecular Function: serine-type endopeptidase inhibitor activity; aspartic-type endopeptidase inhibitor activity; endopeptidase inhibitor activity; cysteine protease inhibitor activity

Biological Process: spermatogenesis; proteolysis
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 Research Articles on HE-4    1. findings implicate increased HE4 expression as a molecular factor in ovarian cancer tumorigenesis
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Diseases associated with HE-4 elisa kitOrgans/Tissues associated with HE-4 elisa kit
 Disease Name  Pubmed Publications
 Ovarian Neoplasms Antibodies  >90 publications with HE-4 and Ovarian Neoplasms
 Carcinoma Antibodies  >39 publications with HE-4 and Carcinoma
 Endometrial Neoplasms Antibodies  >22 publications with HE-4 and Endometrial Neoplasms
 Adenocarcinoma Antibodies  >20 publications with HE-4 and Adenocarcinoma
 Lung Neoplasms Antibodies  >10 publications with HE-4 and Lung Neoplasms
 Breast Neoplasms Antibodies  >6 publications with HE-4 and Breast Neoplasms
 Infertility, Female Antibodies  >3 publications with HE-4 and Infertility, Female
 Inflammation Antibodies  >2 publications with HE-4 and Inflammation
 Fibrosis Antibodies  >2 publications with HE-4 and Fibrosis
 Pancreatic Neoplasms Antibodies  >2 publications with HE-4 and Pancreatic Neoplasms
 Organ/Tissue Name  Pubmed Publications
 Epididymis Antibodies  >66 publications with HE-4 and Epididymis
 Ovary Antibodies  >13 publications with HE-4 and Ovary
 Lung Antibodies  >10 publications with HE-4 and Lung
 Uterus Antibodies  >6 publications with HE-4 and Uterus
 Prostate Antibodies  >4 publications with HE-4 and Prostate
 Trachea Antibodies  >3 publications with HE-4 and Trachea
 Kidney Antibodies  >2 publications with HE-4 and Kidney
 Mammary Gland Antibodies  >2 publications with HE-4 and Mammary Gland
 Muscle Antibodies  >2 publications with HE-4 and Muscle
 Pituitary Gland Antibodies  >1 publications with HE-4 and Pituitary Gland
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