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E2 elisa kit :: Rat Estradiol  (E2) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS263466
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $290 +1 FREE 8GB USB
96-Strip-Wells  /  $450 +1 FREE 8GB USB
5x96-Strip-Wells  /  $1,755 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,150 +3 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Estradiol  (E2), ELISA Kit

 Also Known As   

Rat Estradiol (E2) ELISA Kit

 Product Gene Name   

E2 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Manual Insert    Download PDF Manual View PDF Manual
 OMIM    109684
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 Species Reactivity    Rat
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 Specificity    No cross-reaction with other factors.
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 Samples    Rat serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant E2 concentration
 Assay Type    Sandwich
 Detection Range    1000 pg/ml-15.6 pg/ml
 Sensitivity    5 pg/ml.
 Intra-assay Precision    <= 8%
 Inter-assay Precision    <= 12%
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Product Note    Our ELISA assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay.
 Other Notes    Small volumes of E2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for E2 purchase    MBS263466 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Estradiol  (E2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 1000 pg/ml-15.6 pg/ml in biological research samples containing E2, with an estimated sensitivity of 5 pg/ml. The ELISA analytical biochemical technique of the MBS263466 kit is based on E2 antibody-E2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect E2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, E2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Rat serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant E2 concentration. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for E2 elisa kit

   Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Rat E2 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
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 Typical Testing Data/Standard Curve (for reference only) of E2 elisa kit    E2 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS263466
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NCBI/Uniprot data below describe general gene information for E2. It may not necessarily be applicable to this product.
 NCBI GI #    223718074
 NCBI GeneID    3292
 NCBI Accession #    NP_000404.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_000413.2 [Other Products]
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 UniProt Secondary Accession #    Q2M2L8; B3KXS1 [Other Products]
 UniProt Related Accession #    P14061 [Other Products]
 Molecular Weight    34,950 Da
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 NCBI Official Full Name    estradiol 17-beta-dehydrogenase 1
 NCBI Official Synonym Full Names    hydroxysteroid (17-beta) dehydrogenase 1
 NCBI Official Symbol    HSD17B1 [Similar Products]
 NCBI Official Synonym Symbols   
HSD17; EDHB17; EDH17B2; SDR28C1
[Similar Products]
 NCBI Protein Information    estradiol 17-beta-dehydrogenase 1; E2DH; 20-alpha-HSD; 17-beta-HSD 1; estradiol 17-beta-dehydrogenase-1; 20 alpha-hydroxysteroid dehydrogenase; 17-beta-hydroxysteroid dehydrogenase type 1; placental 17-beta-hydroxysteroid dehydrogenase; hydroxysteroid (17-beta) dehydrogenase 1 isoform; short chain dehydrogenase/reductase family 28CE, member 1
 UniProt Protein Name    Estradiol 17-beta-dehydrogenase 1
 UniProt Synonym Protein Names   
17-beta-hydroxysteroid dehydrogenase type 1; 17-beta-HSD 1; 20 alpha-hydroxysteroid dehydrogenase; 20-alpha-HSD; E2DH; Placental 17-beta-hydroxysteroid dehydrogenase
 Protein Family    Regulatory protein
 UniProt Gene Name    HSD17B1 [Similar Products]
 UniProt Synonym Gene Names    E17KSR; EDH17B1; EDH17B2; EDHB17; 17-beta-HSD 1; 20-alpha-HSD [Similar Products]
 UniProt Entry Name    DHB1_HUMAN
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 UniProt Comments for E2    HSD17B1: Favors the reduction of estrogens and androgens. Also has 20-alpha-HSD activity. Uses preferentially NADH. Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Protein type: EC 1.1.1.62; Oxidoreductase; Lipid Metabolism - androgen and estrogen

Chromosomal Location of Human Ortholog: 17q11-q21

Cellular Component: nucleoplasm; nuclear membrane; cytoplasm; cytosol

Molecular Function: estradiol 17-beta-dehydrogenase activity; catalytic activity; 3-alpha(17-beta)-hydroxysteroid dehydrogenase (NAD+) activity

Biological Process: steroid metabolic process; estrogen metabolic process; estrogen biosynthetic process; steroid biosynthetic process
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 Research Articles on E2    1. Onset of epileptiform discharges is modestly delayed in slices from ovariectomized rats replaced with physiologically relevant doses of EB, but the number of discharges is not affected.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with E2 elisa kitPathways associated with E2 elisa kit
 Reference Product  PubMed Publications
 CYP19A1 elisa kit  >49 publications with E2 and CYP19A1
 CYP1A1 elisa kit  >19 publications with E2 and CYP1A1
 CYP1B1 elisa kit  >18 publications with E2 and CYP1B1
 UGT2B15 elisa kit  >3 publications with E2 and UGT2B15
 SULT1E1 elisa kit  >3 publications with E2 and SULT1E1
 CYP3A4 elisa kit  >3 publications with E2 and CYP3A4
 UGT2B17 elisa kit  >2 publications with E2 and UGT2B17
 Products by Pathway  Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Diseases Associated With Visual Transduction Pathway antibodies  Diseases Associated With Visual Transduction Pathway Diagram
 Estrogen Biosynthesis Pathway antibodies  Estrogen Biosynthesis Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Lipids And Lipoproteins Pathway antibodies  Metabolism Of Lipids And Lipoproteins Pathway Diagram
 Metabolism Of Steroid Hormones And Vitamin D Pathway antibodies  Metabolism Of Steroid Hormones And Vitamin D Pathway Diagram
 Ovarian Steroidogenesis Pathway antibodies  Ovarian Steroidogenesis Pathway Diagram
 Ovarian Steroidogenesis Pathway antibodies  Ovarian Steroidogenesis Pathway Diagram
 Prostate Cancer Pathway antibodies  Prostate Cancer Pathway Diagram
Diseases associated with E2 elisa kitOrgans/Tissues associated with E2 elisa kit
 Disease Name  Pubmed Publications
 Breast Neoplasms Antibodies  >35 publications with E2 and Breast Neoplasms
 Endometriosis Antibodies  >16 publications with E2 and Endometriosis
 Genital Diseases, Male Antibodies  >11 publications with E2 and Genital Diseases, Male
 Adenocarcinoma Antibodies  >9 publications with E2 and Adenocarcinoma
 Prostatic Diseases Antibodies  >7 publications with E2 and Prostatic Diseases
 Prostatic Neoplasms Antibodies  >7 publications with E2 and Prostatic Neoplasms
 Congenital Abnormalities Antibodies  >6 publications with E2 and Congenital Abnormalities
 Urogenital Abnormalities Antibodies  >5 publications with E2 and Urogenital Abnormalities
 Disorders of Sex Development Antibodies  >4 publications with E2 and Disorders of Sex Development
 Ovarian Diseases Antibodies  >4 publications with E2 and Ovarian Diseases
 Organ/Tissue Name  Pubmed Publications
 Placenta Antibodies  >15 publications with E2 and Placenta
 Brain Antibodies  >7 publications with E2 and Brain
 Uterus Antibodies  >5 publications with E2 and Uterus
 Embryonic Tissue Antibodies  >5 publications with E2 and Embryonic Tissue
 Mammary Gland Antibodies  >4 publications with E2 and Mammary Gland
 Testis Antibodies  >4 publications with E2 and Testis
 Stomach Antibodies  >3 publications with E2 and Stomach
 Bone Antibodies  >2 publications with E2 and Bone
 Intestine Antibodies  >1 publications with E2 and Intestine
 Kidney Antibodies  >1 publications with E2 and Kidney
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