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E2 elisa kit :: Rat Estradiol, E2 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS040105 E2 elisa kit
Unit / Price
48-Strip-Wells  /  $435 +1 FREE 8GB USB
96-Strip-Wells  /  $600 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,650 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,205 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Estradiol, E2, ELISA Kit

★Popular Item★
 Also Known As   

Rat Estradiol, E2 ELISA Kit

 Product Gene Name   

E2 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    609574
 3D Structure    ModBase 3D Structure for Q53GQ0
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 Species Reactivity    Rat
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 Specificity    No significant cross-reactivity or interference between this analyte and analogues is observed.
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 Assay Type    Quantitative Sandwich
 Detection Range    6.25 pg/ml - 200 pg/ml
 Sensitivity    1.0 pg/ml
 Intra-assay Precision    Intra-assay CV (%) is less than 15%.
 Inter-assay Precision    Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean 100].
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 Preparation and Storage    Store all reagents at 2-8 degree C
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of E2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for E2 purchase    MBS040105 is a ready-to-use microwell, strip plate Quantitative Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Estradiol, E2, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 6.25 pg/ml - 200 pg/ml in biological research samples containing E2, with an estimated sensitivity of 1.0 pg/ml. The ELISA analytical biochemical technique of the MBS040105 kit is based on E2 antibody-E2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect E2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, E2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for E2 elisa kit

   Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! This kit is intended to be used for determination the level of E2 (hereafter termed "analyte") in undiluted original Rat body fluids, tissue homogenates, secretions or feces samples. This kit is NOT suitable for assaying non-biological sources of substances.
Sample Manual Insert of MBS040105. Click to request current manual
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NCBI/Uniprot data below describe general gene information for E2. It may not necessarily be applicable to this product.
 NCBI GI #    7705855
 NCBI GeneID    51144
 NCBI Accession #    NP_057226.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_016142.2 [Other Products]
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 UniProt Primary Accession #    Q53GQ0 [Other Products]
 UniProt Secondary Accession #    Q96JU2; Q9Y6G8; A8K9B0; D3DR23 [Other Products]
 UniProt Related Accession #    Q53GQ0 [Other Products]
 Molecular Weight    34,324 Da
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 NCBI Official Full Name    estradiol 17-beta-dehydrogenase 12
 NCBI Official Synonym Full Names    hydroxysteroid (17-beta) dehydrogenase 12
 NCBI Official Symbol    HSD17B12 [Similar Products]
 NCBI Official Synonym Symbols   
KAR; SDR12C1
[Similar Products]
 NCBI Protein Information    estradiol 17-beta-dehydrogenase 12; 17-beta-HSD 12; 17beta-HSD type 12; 3-ketoacyl-CoA reductase; steroid dehydrogenase homolog; 17-beta-hydroxysteroid dehydrogenase 12; short chain dehydrogenase/reductase family 12C, member 1
 UniProt Protein Name    Estradiol 17-beta-dehydrogenase 12
 UniProt Synonym Protein Names   
17-beta-hydroxysteroid dehydrogenase 12; 17-beta-HSD 12; 3-ketoacyl-CoA reductase
 Protein Family    Regulatory protein
 UniProt Gene Name    HSD17B12 [Similar Products]
 UniProt Synonym Gene Names    17-beta-HSD 12; KAR [Similar Products]
 UniProt Entry Name    DHB12_HUMAN
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 NCBI Summary for E2    This gene encodes a very important 17beta-hydroxysteroid dehydrogenase (17beta-HSD) that converts estrone into estradiol in ovarian tissue. This enzyme is also involved in fatty acid elongation. [provided by RefSeq, Oct 2011]
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 UniProt Comments for E2    HSD17B12: Catalyzes the transformation of estrone (E1) into estradiol (E2), suggesting a central role in estrogen formation. Its strong expression in ovary and mammary gland suggest that it may constitute the major enzyme responsible for the conversion of E1 to E2 in women. Also has 3-ketoacyl-CoA reductase activity, reducing both long chain 3-ketoacyl-CoAs and long chain fatty acyl-CoAs, suggesting a role in long fatty acid elongation. Belongs to the short-chain dehydrogenases/reductases (SDR) family. 17-beta-HSD 3 subfamily.

Protein type: Lipid Metabolism - androgen and estrogen; EC 1.1.1.62; Membrane protein, integral; Membrane protein, multi-pass; Lipid Metabolism - unsaturated fatty acid biosynthesis; Oxidoreductase

Chromosomal Location of Human Ortholog: 11p11.2

Cellular Component: extracellular matrix; endoplasmic reticulum membrane; integral to membrane

Molecular Function: estradiol 17-beta-dehydrogenase activity; collagen binding; heparin binding; protein binding; fibronectin binding

Biological Process: extracellular matrix organization and biogenesis; triacylglycerol biosynthetic process; cellular lipid metabolic process; estrogen biosynthetic process; fatty acid biosynthetic process
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 Research Articles on E2    1. Significant associations have been found between CpG sites and patient sex, including DNA methylation in CASP6, a gene that may respond to estradiol treatment, and in HSD17B12, which encodes a sex steroid hormone.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with E2 elisa kitDiseases associated with E2 elisa kit
 Products by Pathway  Pathway Diagram
 Androgen Biosynthesis Pathway antibodies  Androgen Biosynthesis Pathway Diagram
 Biosynthesis Of Unsaturated Fatty Acids Pathway antibodies  Biosynthesis Of Unsaturated Fatty Acids Pathway Diagram
 Biosynthesis Of Unsaturated Fatty Acids Pathway antibodies  Biosynthesis Of Unsaturated Fatty Acids Pathway Diagram
 Fatty Acyl-CoA Biosynthesis Pathway antibodies  Fatty Acyl-CoA Biosynthesis Pathway Diagram
 Fatty Acid Biosynthesis, Elongation, Endoplasmic Reticulum Pathway antibodies  Fatty Acid Biosynthesis, Elongation, Endoplasmic Reticulum Pathway Diagram
 Fatty Acid Biosynthesis, Elongation, Endoplasmic Reticulum Pathway antibodies  Fatty Acid Biosynthesis, Elongation, Endoplasmic Reticulum Pathway Diagram
 Fatty Acid Elongation Pathway antibodies  Fatty Acid Elongation Pathway Diagram
 Fatty Acid Elongation Pathway antibodies  Fatty Acid Elongation Pathway Diagram
 Fatty Acid Metabolism Pathway antibodies  Fatty Acid Metabolism Pathway Diagram
 Fatty Acid Metabolism Pathway antibodies  Fatty Acid Metabolism Pathway Diagram
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >11 publications with E2 and Neoplasms
 Breast Neoplasms Antibodies  >4 publications with E2 and Breast Neoplasms
 Neoplasms, Experimental Antibodies  >3 publications with E2 and Neoplasms, Experimental
 Prostatic Neoplasms Antibodies  >3 publications with E2 and Prostatic Neoplasms
 Lung Diseases Antibodies  >2 publications with E2 and Lung Diseases
 Inflammation Antibodies  >2 publications with E2 and Inflammation
 Atrophy Antibodies  >1 publications with E2 and Atrophy
 Lung Neoplasms Antibodies  >1 publications with E2 and Lung Neoplasms
 Heart Diseases Antibodies  >1 publications with E2 and Heart Diseases
 Cardiovascular Diseases Antibodies  >1 publications with E2 and Cardiovascular Diseases
Organs/Tissues associated with E2 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >9 publications with E2 and Blood
 Prostate Antibodies  >3 publications with E2 and Prostate
 Lung Antibodies  >2 publications with E2 and Lung
 Heart Antibodies  >1 publications with E2 and Heart
 Muscle Antibodies  >1 publications with E2 and Muscle
 Vascular Antibodies  >1 publications with E2 and Vascular
 Embryonic Tissue Antibodies  >1 publications with E2 and Embryonic Tissue
 Uncharacterized Tissue Antibodies  >1 publications with E2 and Uncharacterized Tissue
 Lymph Node Antibodies  >1 publications with E2 and Lymph Node
 Lymph Antibodies  >1 publications with E2 and Lymph
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