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EIF2A elisa kit :: Rat Eukaryotic Translation Initiation Factor 2A ELISA Kit

Scan QR to view Datasheet Catalog #    MBS751823
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $440 +1 FREE 8GB USB
96-Strip-Wells  /  $640 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Eukaryotic Translation Initiation Factor 2A (EIF2A), ELISA Kit

★Popular Item★
 Also Known As   

Rat Eukaryotic Translation Initiation Factor 2A ELISA Kit

 Product Gene Name   

EIF2A elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    609234
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 Species Reactivity    Rat
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 Specificity    This assay has high sensitivity and excellent specificity for detection of EIF-2A. No significant cross-reactivity or interference between EIF-2A and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between EIF-2A and all the analogues, therefore, cross reaction may still exist in some cases.
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 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Assay Type    Competitive
 Detection Range    100-2500pg/mL
 Sensitivity    1.0pg/ml
 Intended Uses    This EIF-2A ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat EIF-2A. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of EIF2A elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for EIF2A purchase    MBS751823 is a ready-to-use microwell, strip plate Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Eukaryotic Translation Initiation Factor 2A (EIF2A) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 100-2500pg/mL in biological research samples containing EIF2A, with an estimated sensitivity of 1.0pg/ml. The ELISA analytical biochemical technique of the MBS751823 kit is based on EIF2A antibody-EIF2A antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect EIF2A antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, EIF2A. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for EIF2A elisa kit

  
Principle of the assay: EIF-2A ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for EIF-2A. Standards or samples are then added to the microtiter plate wells and EIF-2A if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of EIF-2A present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for EIF-2A are added to each well to "sandwich" the EIF-2A immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain EIF-2A and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The EIF-2A concentration in each sample is interpolated from this standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of EIF2A elisa kit    EIF2A elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS751823. Click to request current manual
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NCBI/Uniprot data below describe general gene information for EIF2A. It may not necessarily be applicable to this product.
 NCBI GI #    54873624
 NCBI GeneID    83939
 NCBI Accession #    NP_114414.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_032025.3 [Other Products]
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 UniProt Secondary Accession #    Q5QTR2; Q7Z4E9; Q8NFM1; Q96EW9; Q96K81; A8MPS6; D3DNI9 [Other Products]
 UniProt Related Accession #    Q9BY44 [Other Products]
 Molecular Weight    41,339 Da
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 NCBI Official Full Name    eukaryotic translation initiation factor 2A
 NCBI Official Synonym Full Names    eukaryotic translation initiation factor 2A, 65kDa
 NCBI Official Symbol    EIF2A [Similar Products]
 NCBI Official Synonym Symbols   
CDA02; EIF-2A; MST089; MSTP004; MSTP089
[Similar Products]
 NCBI Protein Information    eukaryotic translation initiation factor 2A; 65 kDa eukaryotic translation initiation factor 2A
 UniProt Protein Name    Eukaryotic translation initiation factor 2A
 UniProt Synonym Protein Names   
65 kDa eukaryotic translation initiation factor 2ACleaved into the following chain:Eukaryotic translation initiation factor 2A, N-terminally processed
 Protein Family    Eukaryotic translation initiation factor
 UniProt Gene Name    EIF2A [Similar Products]
 UniProt Synonym Gene Names    CDA02; MSTP004; MSTP089; eIF-2A [Similar Products]
 UniProt Entry Name    EIF2A_HUMAN
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 NCBI Summary for EIF2A    EIF2A is a 65-kD protein that catalyzes the formation of puromycin-sensitive 80S preinitiation complexes (Zoll et al., 2002 [PubMed 12133843]).[supplied by OMIM, Mar 2008]
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 UniProt Comments for EIF2A    EIF2A: Functions in the early steps of protein synthesis of a small number of specific mRNAs. Acts by directing the binding of methionyl-tRNAi to 40S ribosomal subunits. In contrast to the eIF- 2 complex, it binds methionyl-tRNAi to 40 S subunits in a codon- dependent manner, whereas the eIF-2 complex binds methionyl-tRNAi to 40 S subunits in a GTP-dependent manner. May act by impiging the expression of specific proteins. Belongs to the WD repeat EIF2A family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Translation

Chromosomal Location of Human Ortholog: 3q25.1

Cellular Component: extracellular space; cytoplasm; eukaryotic translation initiation factor 2 complex

Molecular Function: protein binding; translation initiation factor activity; ribosome binding; tRNA binding

Biological Process: regulation of translation; positive regulation of signal transduction; ribosome assembly; translational initiation; SREBP-mediated signaling pathway; protein amino acid phosphorylation
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 Research Articles on EIF2A    1. PKR is activated by dsRNA generated by Newcastle disease virus infection and inhibits Newcastle disease virus replication by eIF2alpha phosphorylation.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with EIF2A elisa kitPathways associated with EIF2A elisa kit
 Reference Product  PubMed Publications
 ATF4 elisa kit  >4 publications with EIF2A and ATF4
 EIF5B elisa kit  >1 publications with EIF2A and EIF5B
 Products by Pathway  Pathway Diagram
 IL-6 Signaling Pathway antibodies  IL-6 Signaling Pathway Diagram
 TGF-beta Receptor Signaling Pathway antibodies  TGF-beta Receptor Signaling Pathway Diagram
Diseases associated with EIF2A elisa kitOrgans/Tissues associated with EIF2A elisa kit
 Disease Name  Pubmed Publications
 Liver Diseases Antibodies  >4 publications with EIF2A and Liver Diseases
 Nervous System Diseases Antibodies  >3 publications with EIF2A and Nervous System Diseases
 Inflammation Antibodies  >2 publications with EIF2A and Inflammation
 Gastrointestinal Diseases Antibodies  >2 publications with EIF2A and Gastrointestinal Diseases
 Necrosis Antibodies  >2 publications with EIF2A and Necrosis
 Atherosclerosis Antibodies  >1 publications with EIF2A and Atherosclerosis
 Fetal Growth Retardation Antibodies  >1 publications with EIF2A and Fetal Growth Retardation
 Cell Transformation, Neoplastic Antibodies  >1 publications with EIF2A and Cell Transformation, Neoplastic
 Fibrosis Antibodies  >1 publications with EIF2A and Fibrosis
 Lung Neoplasms Antibodies  >1 publications with EIF2A and Lung Neoplasms
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >5 publications with EIF2A and Brain
 Lung Antibodies  >3 publications with EIF2A and Lung
 Liver Antibodies  >3 publications with EIF2A and Liver
 Thyroid Antibodies  >2 publications with EIF2A and Thyroid
 Pancreas Antibodies  >2 publications with EIF2A and Pancreas
 Adipose Tissue Antibodies  >2 publications with EIF2A and Adipose Tissue
 Muscle Antibodies  >2 publications with EIF2A and Muscle
 Blood Antibodies  >2 publications with EIF2A and Blood
 Intestine Antibodies  >1 publications with EIF2A and Intestine
 Bladder Antibodies  >1 publications with EIF2A and Bladder
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