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EAAT1 elisa kit :: Rat Excitatory Amino Acid Transporter 1 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS050004 EAAT1 elisa kit
Unit / Price
48-Strip-Wells  /  $470 +1 FREE 8GB USB
96-Strip-Wells  /  $680 +1 FREE 8GB USB
5x96-Strip-Wells  /  $3,100 +3 FREE 8GB USB
10x96-Strip-Wells  /  $6,095 +7 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Excitatory Amino Acid Transporter 1 (EAAT1), ELISA Kit

★Popular Item★
 Also Known As   

Rat Excitatory Amino Acid Transporter 1 ELISA Kit

 Product Gene Name   

EAAT1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    600111
 3D Structure    ModBase 3D Structure for P43003
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 Species Reactivity    Rat
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 Specificity    No significant cross-reactivity or interference between Rat EAAT1 and analogues was observed.
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 Samples    Serum, Plasma, Tissue Homogenate, Feces and Urine
 Assay Type    Sandwich
 Detection Range    0.25 ng/ml - 8 ng/ml.
 Sensitivity    0.1 ng/ml.
 Intended Uses    This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated EAAT1 concentrations in Rat serum, plasma and other body fluids. Using Purified Rat EAAT1 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add EAAT1 and EAAT1 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of EAAT1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
 Intra-assay Precision    Intra-assay CV (%) is less than 15%.
 Inter-assay Precision    Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean 100]
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 Preparation and Storage    Store all reagents at 2-8 degree C
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of EAAT1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for EAAT1 purchase    MBS050004 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Excitatory Amino Acid Transporter 1 (EAAT1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.25 ng/ml - 8 ng/ml in biological research samples containing EAAT1, with an estimated sensitivity of 0.1 ng/ml. The ELISA analytical biochemical technique of the MBS050004 kit is based on EAAT1 antibody-EAAT1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect EAAT1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, EAAT1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma, Tissue Homogenate, Feces and Urine. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for EAAT1 elisa kit

   Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated EAAT1 concentrations in Rat serum, plasma and other body fluids. Using Purified Rat EAAT1 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add EAAT1 and EAAT1 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of EAAT1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Sample Manual Insert of MBS050004. Click to request current manual
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NCBI/Uniprot data below describe general gene information for EAAT1. It may not necessarily be applicable to this product.
 NCBI GI #    584277111
 NCBI GeneID    6507
 NCBI Accession #    NP_001276868.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001289939.1 [Other Products]
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 UniProt Primary Accession #    P43003 [Other Products]
 UniProt Secondary Accession #    Q4JCQ8; B2R5T3 [Other Products]
 UniProt Related Accession #    P43003 [Other Products]
 Molecular Weight    59,572 Da
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 NCBI Official Full Name    excitatory amino acid transporter 1 isoform 4
 NCBI Official Synonym Full Names    solute carrier family 1 (glial high affinity glutamate transporter), member 3
 NCBI Official Symbol    SLC1A3 [Similar Products]
 NCBI Official Synonym Symbols   
EA6; EAAT1; GLAST; GLAST1
[Similar Products]
 NCBI Protein Information    excitatory amino acid transporter 1; GLAST-1; solute carrier family 1 member 3; sodium-dependent glutamate/aspartate transporter 1
 UniProt Protein Name    Excitatory amino acid transporter 1
 UniProt Synonym Protein Names   
Sodium-dependent glutamate/aspartate transporter 1; GLAST-1; Solute carrier family 1 member 3
 Protein Family    Excitatory amino acid transporter
 UniProt Gene Name    SLC1A3 [Similar Products]
 UniProt Synonym Gene Names    EAAT1; GLAST; GLAST1; GLAST-1 [Similar Products]
 UniProt Entry Name    EAA1_HUMAN
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 NCBI Summary for EAAT1    This gene encodes a member of a member of a high affinity glutamate transporter family. This gene functions in the termination of excitatory neurotransmission in central nervous system. Mutations are associated with episodic ataxia, Type 6. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Feb 2014]
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 UniProt Comments for EAAT1    SLC1A3: Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium. Highly expressed in cerebellum, but also found in frontal cortex, hippocampus and basal ganglia. Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family. SLC1A3 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transporter; Mitochondrial; Transporter, SLC family; Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 5p13

Cellular Component: cell surface; cell projection; membrane; cell soma; fibril; integral to membrane; plasma membrane

Molecular Function: glutamate binding; high-affinity glutamate transmembrane transporter activity; sodium:dicarboxylate symporter activity; L-glutamate transmembrane transporter activity

Biological Process: response to drug; response to light stimulus; cranial nerve development; auditory behavior; glutamate biosynthetic process; positive regulation of synaptic transmission; response to antibiotic; L-glutamate import; synaptic transmission; sensory perception of sound; neuron morphogenesis during differentiation; gamma-aminobutyric acid biosynthetic process; response to wounding; neurotransmitter uptake; ion transport; neuromuscular process controlling balance; transmembrane transport

Disease: Episodic Ataxia, Type 6
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 Research Articles on EAAT1    1. Increased SLC1A3 expression in the cerebellum of elderly schizophrenia patients indicates facilitated transport and may result in reduced glutamate neurotransmission.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with EAAT1 elisa kitPathways associated with EAAT1 elisa kit
 Reference Product  PubMed Publications
 GFAP elisa kit  >40 publications with EAAT1 and GFAP
 GLUL elisa kit  >5 publications with EAAT1 and GLUL
 SLC2A1 elisa kit  >4 publications with EAAT1 and SLC2A1
 GDNF elisa kit  >4 publications with EAAT1 and GDNF
 Products by Pathway  Pathway Diagram
 Astrocytic Glutamate-Glutamine Uptake And Metabolism Pathway antibodies  Astrocytic Glutamate-Glutamine Uptake And Metabolism Pathway Diagram
 Glutamatergic Synapse Pathway antibodies  Glutamatergic Synapse Pathway Diagram
 Glutamatergic Synapse Pathway antibodies  Glutamatergic Synapse Pathway Diagram
 Neuronal System Pathway antibodies  Neuronal System Pathway Diagram
 Neurotransmitter Uptake And Metabolism In Glial Cells Pathway antibodies  Neurotransmitter Uptake And Metabolism In Glial Cells Pathway Diagram
 SIDS Susceptibility Pathways antibodies  SIDS Susceptibility Pathways Diagram
 SLC-mediated Transmembrane Transport Pathway antibodies  SLC-mediated Transmembrane Transport Pathway Diagram
 Synaptic Vesicle Pathway antibodies  Synaptic Vesicle Pathway Diagram
 Transmembrane Transport Of Small Molecules Pathway antibodies  Transmembrane Transport Of Small Molecules Pathway Diagram
 Transmission Across Chemical Synapses Pathway antibodies  Transmission Across Chemical Synapses Pathway Diagram
Diseases associated with EAAT1 elisa kitOrgans/Tissues associated with EAAT1 elisa kit
 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >107 publications with EAAT1 and Nervous System Diseases
 Brain Diseases Antibodies  >76 publications with EAAT1 and Brain Diseases
 Disease Models, Animal Antibodies  >53 publications with EAAT1 and Disease Models, Animal
 Cardiovascular Diseases Antibodies  >32 publications with EAAT1 and Cardiovascular Diseases
 Neoplasms Antibodies  >21 publications with EAAT1 and Neoplasms
 Epilepsy Antibodies  >17 publications with EAAT1 and Epilepsy
 Nerve Degeneration Antibodies  >13 publications with EAAT1 and Nerve Degeneration
 Seizures Antibodies  >13 publications with EAAT1 and Seizures
 Movement Disorders Antibodies  >11 publications with EAAT1 and Movement Disorders
 Inflammation Antibodies  >9 publications with EAAT1 and Inflammation
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >235 publications with EAAT1 and Brain
 Nerve Antibodies  >69 publications with EAAT1 and Nerve
 Eye Antibodies  >47 publications with EAAT1 and Eye
 Blood Antibodies  >30 publications with EAAT1 and Blood
 Spinal Cord Antibodies  >28 publications with EAAT1 and Spinal Cord
 Embryonic Tissue Antibodies  >19 publications with EAAT1 and Embryonic Tissue
 Muscle Antibodies  >11 publications with EAAT1 and Muscle
 Kidney Antibodies  >11 publications with EAAT1 and Kidney
 Heart Antibodies  >7 publications with EAAT1 and Heart
 Liver Antibodies  >6 publications with EAAT1 and Liver
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