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FADS1 elisa kit :: Rat Fatty Acid Desaturase 1 (FADS1) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2019105 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells  /  $405 +1 FREE 8GB USB
48-Strip-Wells  /  $605 +1 FREE 8GB USB
96-Strip-Wells  /  $820 +1 FREE 8GB USB
5x96-Strip-Wells  /  $3,375 +4 FREE 8GB USB
10x96-Strip-Wells  /  $6,125 +7 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Fatty Acid Desaturase 1 (FADS1), ELISA Kit

 Also Known As   

Fatty Acid Desaturase 1 (FADS1) ELISA Kit

 Product Synonym Names    D5D; FADS6; FADSD5; LLCDL1; TU12; Delta-5 Desaturase; Delta(5) fatty acid desaturase
 Product Gene Name   

FADS1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    606148
 3D Structure    ModBase 3D Structure for O60427
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 Species Reactivity    Rat
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Fatty Acid Desaturase 1 (FADS1).
No significant cross-reactivity or interference between Fatty Acid Desaturase 1 (FADS1) and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates and other biological fluids
 Assay Type    Sandwich
 Detection Range    62.5-4000pg/mL
 Sensitivity    Typically less than 22.4pg/mL.
 Precision    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fatty Acid Desaturase 1 (FADS1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fatty Acid Desaturase 1 (FADS1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
 Assay Procedure Summary    1. Prepare all reagents, samples and standards;
2. Add 100uL standard or sample to each well. Incubate 2 hours at 37 degree C;
3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37 degree C;
4. Aspirate and wash 3 times;
5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37 degree C;
6. Aspirate and wash 5 times;
7. Add 90uL Substrate Solution. Incubate 15-25 minutes at 37 degree C;
8. Add 50uL Stop Solution. Read at 450nm immediately.
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 Preparation and Storage    For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C.

For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
 Product Note    Our ELISA assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of FADS1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for FADS1 purchase    MBS2019105 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Fatty Acid Desaturase 1 (FADS1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 62.5-4000pg/mL in biological research samples containing FADS1, with an estimated sensitivity of Typically less than 22.4pg/mL. The ELISA analytical biochemical technique of the MBS2019105 kit is based on FADS1 antibody-FADS1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect FADS1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, FADS1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for FADS1 elisa kit

   Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fatty Acid Desaturase 1 (FADS1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Fatty Acid Desaturase 1 (FADS1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Fatty Acid Desaturase 1 (FADS1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Fatty Acid Desaturase 1 (FADS1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of FADS1 elisa kit    FADS1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2019105. Click to request current manual
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NCBI/Uniprot data below describe general gene information for FADS1. It may not necessarily be applicable to this product.
 NCBI GI #    214831730
 NCBI GeneID    3992
 NCBI Accession #    NP_037534.3 [Other Products]
 NCBI GenBank Nucleotide #    NM_013402.4 [Other Products]
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 UniProt Primary Accession #    O60427 [Other Products]
 UniProt Secondary Accession #    Q53GM5; Q8N3A6; Q8NCC7; Q8NCG0; Q96I39; Q96SV3; Q96T10; Q9NRP8; Q9NYX1; A8K0I7; B2RAI0 [Other Products]
 UniProt Related Accession #    O60427 [Other Products]
 Molecular Weight    42,474 Da
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 NCBI Official Full Name    fatty acid desaturase 1
 NCBI Official Synonym Full Names    fatty acid desaturase 1
 NCBI Official Symbol    FADS1 [Similar Products]
 NCBI Official Synonym Symbols   
D5D; TU12; FADS6; FADSD5; LLCDL1
[Similar Products]
 NCBI Protein Information    fatty acid desaturase 1
 UniProt Protein Name    Fatty acid desaturase 1
 UniProt Synonym Protein Names   
Delta(5) fatty acid desaturase; D5D; Delta(5) desaturase; Delta-5 desaturase
 Protein Family    Fatty acid desaturase
 UniProt Gene Name    FADS1 [Similar Products]
 UniProt Synonym Gene Names    FADSD5; D5D; Delta(5) desaturase; Delta-5 desaturase [Similar Products]
 UniProt Entry Name    FADS1_HUMAN
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 NCBI Summary for FADS1    The protein encoded by this gene is a member of the fatty acid desaturase (FADS) gene family. Desaturase enzymes regulate unsaturation of fatty acids through the introduction of double bonds between defined carbons of the fatty acyl chain. FADS family members are considered fusion products composed of an N-terminal cytochrome b5-like domain and a C-terminal multiple membrane-spanning desaturase portion, both of which are characterized by conserved histidine motifs. This gene is clustered with family members FADS1 and FADS2 at 11q12-q13.1; this cluster is thought to have arisen evolutionarily from gene duplication based on its similar exon/intron organization. [provided by RefSeq, Jul 2008]
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 UniProt Comments for FADS1    FADS1: Component of a lipid metabolic pathway that catalyzes biosynthesis of highly unsaturated fatty acids (HUFA) from precursor essential polyunsaturated fatty acids (PUFA) linoleic acid (LA) (18:2n-6) and alpha-linolenic acid (ALA) (18:3n-3). Catalyzes the desaturation of dihomo-gamma-linoleic acid (DHGLA) (20:3n-6) and eicosatetraenoic acid (20:4n-3) to generate arachidonic acid (AA) (20:4n-6) and eicosapentaenoic acid (EPA)(20:5n-3) respectively. Belongs to the fatty acid desaturase family.

Protein type: EC 1.14.19.-; Oxidoreductase; Lipid Metabolism - unsaturated fatty acid biosynthesis; Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 11q12.2-q13.1

Cellular Component: endoplasmic reticulum membrane; mitochondrion; intracellular membrane-bound organelle; membrane; integral to membrane

Molecular Function: C-5 sterol desaturase activity; iron ion binding; heme binding; oxidoreductase activity

Biological Process: icosanoid biosynthetic process; cell-cell signaling; regulation of transcription, DNA-dependent; regulation of cell differentiation; unsaturated fatty acid metabolic process; linoleic acid metabolic process; unsaturated fatty acid biosynthetic process; cellular lipid metabolic process; cellular response to starvation; phospholipid biosynthetic process
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 Research Articles on FADS1    1. rare alleles of SNPs significantly associated with the accumulation of multiple long-chain fatty acids in the liver
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with FADS1 elisa kitDiseases associated with FADS1 elisa kit
 Products by Pathway  Pathway Diagram
 Biosynthesis Of Unsaturated Fatty Acids Pathway antibodies  Biosynthesis Of Unsaturated Fatty Acids Pathway Diagram
 Biosynthesis Of Unsaturated Fatty Acids Pathway antibodies  Biosynthesis Of Unsaturated Fatty Acids Pathway Diagram
 Fatty Acid Metabolism Pathway antibodies  Fatty Acid Metabolism Pathway Diagram
 Fatty Acid Metabolism Pathway antibodies  Fatty Acid Metabolism Pathway Diagram
 Fatty Acid, Triacylglycerol, And Ketone Body Metabolism Pathway antibodies  Fatty Acid, Triacylglycerol, And Ketone Body Metabolism Pathway Diagram
 Linoleic Acid (LA) Metabolism Pathway antibodies  Linoleic Acid (LA) Metabolism Pathway Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Lipids And Lipoproteins Pathway antibodies  Metabolism Of Lipids And Lipoproteins Pathway Diagram
 PPARA Activates Gene Expression Pathway antibodies  PPARA Activates Gene Expression Pathway Diagram
 Regulation Of Lipid Metabolism By Peroxisome Proliferator-activated Receptor Alpha (PPARalpha) Pathway antibodies  Regulation Of Lipid Metabolism By Peroxisome Proliferator-activated Receptor Alpha (PPARalpha) Pathway Diagram
 Disease Name  Pubmed Publications
 Fatty Liver Antibodies  >25 publications with FADS1 and Fatty Liver
 Cardiovascular Diseases Antibodies  >23 publications with FADS1 and Cardiovascular Diseases
 Inflammation Antibodies  >22 publications with FADS1 and Inflammation
 Heart Diseases Antibodies  >11 publications with FADS1 and Heart Diseases
 Hypertension Antibodies  >7 publications with FADS1 and Hypertension
 Hyperglycemia Antibodies  >6 publications with FADS1 and Hyperglycemia
 Liver Diseases Antibodies  >5 publications with FADS1 and Liver Diseases
 Atherosclerosis Antibodies  >4 publications with FADS1 and Atherosclerosis
 Disease Models, Animal Antibodies  >4 publications with FADS1 and Disease Models, Animal
 Depressive Disorder Antibodies  >3 publications with FADS1 and Depressive Disorder
Organs/Tissues associated with FADS1 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >85 publications with FADS1 and Blood
 Liver Antibodies  >32 publications with FADS1 and Liver
 Heart Antibodies  >17 publications with FADS1 and Heart
 Adipose Tissue Antibodies  >13 publications with FADS1 and Adipose Tissue
 Brain Antibodies  >11 publications with FADS1 and Brain
 Muscle Antibodies  >9 publications with FADS1 and Muscle
 Connective Tissue Antibodies  >9 publications with FADS1 and Connective Tissue
 Vascular Antibodies  >4 publications with FADS1 and Vascular
 Prostate Antibodies  >3 publications with FADS1 and Prostate
 Eye Antibodies  >3 publications with FADS1 and Eye
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