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FADS2 elisa kit :: Rat Fatty Acid Desaturase 2 (FADS2) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2022791 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells  /  $285 +1 FREE 8GB USB
48-Strip-Wells  /  $530 +1 FREE 8GB USB
96-Strip-Wells  /  $715 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,910 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,280 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Fatty Acid Desaturase 2 (FADS2), ELISA Kit

 Also Known As   

Fatty Acid Desaturase 2 (FADS2) ELISA Kit

 Product Synonym Names    LLCDL2; FADSD6; D6D; TU13; DES6; Delta-6-Desaturase; Delta(6) fatty acid desaturase
 Product Gene Name   

FADS2 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 3D Structure    ModBase 3D Structure for Q9Z122
 Species Reactivity    Rat
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Fatty Acid Desaturase 2 (FADS2).
No significant cross-reactivity or interference between Fatty Acid Desaturase 2 (FADS2) and analogues was observed.
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 Assay Type    Double-antibody Sandwich
 Samples    Serum, Plasma, Tissue homogenates and Other Biological Fluids
 Detection Range    1.56-100ng/mL
 Sensitivity    < 0.64ng/mL
 Application    Enzyme-linked immunosorbent assay for Antigen Detection.
 Intra-assay Precision (Precision within an assay)    3 samples with low, middle and high level Fatty Acid Desaturase 2 (FADS2) were tested 20 times on one plate, respectively.
 Inter-assay Precision (Precision between assays)    3 samples with low, middle and high level Fatty Acid Desaturase 2 (FADS2) were tested on 3 different plates, 8 replicates in each plate.
 CV(%) =    SD/meanX100
 Intra-Assay    CV<10%
 Inter-Assay    CV<12%
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 Preparation and Storage    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
 ISO Certification    Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
 Supply Chain Verification    Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available. Please inquire.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of FADS2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for FADS2 purchase    MBS2022791 is a ready-to-use microwell, strip plate Double-antibody Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Fatty Acid Desaturase 2 (FADS2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 1.56-100ng/mL in biological research samples containing FADS2, with an estimated sensitivity of < 0.64ng/mL. The ELISA analytical biochemical technique of the MBS2022791 kit is based on FADS2 antibody-FADS2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect FADS2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, FADS2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma, Tissue homogenates and Other Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for FADS2 elisa kit

   The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fatty Acid Desaturase 2 (FADS2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Fatty Acid Desaturase 2 (FADS2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Fatty Acid Desaturase 2 (FADS2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Fatty Acid Desaturase 2 (FADS2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
 Product Categories/Family for FADS2 elisa kit    Enzyme & Kinase
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 Typical Testing Data/Standard Curve (for reference only) of FADS2 elisa kit    FADS2 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2022791. Click to request current manual
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NCBI/Uniprot data below describe general gene information for FADS2. It may not necessarily be applicable to this product.
 NCBI GI #    13786182
 NCBI GeneID    83512
 NCBI Accession #    NP_112634.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_031344.2 [Other Products]
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 UniProt Primary Accession #    Q9Z122 [Other Products]
 UniProt Secondary Accession #    H2BF31 [Other Products]
 UniProt Related Accession #    Q9Z122 [Other Products]
 Molecular Weight    52,380 Da
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 NCBI Official Full Name    fatty acid desaturase 2
 NCBI Official Synonym Full Names    fatty acid desaturase 2
 NCBI Official Symbol    Fads2 [Similar Products]
 NCBI Official Synonym Symbols   
Fadsd6
[Similar Products]
 NCBI Protein Information    fatty acid desaturase 2
 UniProt Protein Name    Fatty acid desaturase 2
 UniProt Synonym Protein Names   
Delta(6) fatty acid desaturase; D6D; Delta(6) desaturase; Delta-6 desaturase
 Protein Family    Fatty acid desaturase
 UniProt Gene Name    Fads2 [Similar Products]
 UniProt Synonym Gene Names    Fadsd6; D6D; Delta(6) desaturase; Delta-6 desaturase [Similar Products]
 UniProt Entry Name    FADS2_RAT
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 UniProt Comments for FADS2    Component of a lipid metabolic pathway that catalyzes biosynthesis of highly unsaturated fatty acids (HUFA) from precursor essential polyunsaturated fatty acids (PUFA) linoleic acid (LA) (18:2n-6) and alpha-linolenic acid (ALA) (18:3n-3). Catalyzes the first and rate limiting step in this pathway which is the desaturation of LA (18:2n-6) and ALA (18:3n-3) into gamma-linoleic acid (GLA) (18:3n-6) and stearidonic acid (18:4n-3) respectively and other desaturation steps. Highly unsaturated fatty acids (HUFA) play pivotal roles in many biological functions.
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 Research Articles on FADS2    1. Results show that genes with the highest expression in the mammary gland, liver and adipose tissue were Elovl5, Fads2, and Fasn, respectively, in a low-lipid diet than in adequate-lipid diet.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with FADS2 elisa kitPathways associated with FADS2 elisa kit
 Reference Product  PubMed Publications
 Elovl5 elisa kit  >23 publications with FADS2 and Elovl5
 Acsl1 elisa kit  >2 publications with FADS2 and Acsl1
 Products by Pathway  Pathway Diagram
 Biosynthesis Of Unsaturated Fatty Acids Pathway antibodies  Biosynthesis Of Unsaturated Fatty Acids Pathway Diagram
 Biosynthesis Of Unsaturated Fatty Acids Pathway antibodies  Biosynthesis Of Unsaturated Fatty Acids Pathway Diagram
 Fatty Acid Metabolism Pathway antibodies  Fatty Acid Metabolism Pathway Diagram
 Fatty Acid Metabolism Pathway antibodies  Fatty Acid Metabolism Pathway Diagram
 Folic Acid Network Pathway antibodies  Folic Acid Network Pathway Diagram
 Linoleic Acid (LA) Metabolism Pathway antibodies  Linoleic Acid (LA) Metabolism Pathway Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Lipids And Lipoproteins Pathway antibodies  Metabolism Of Lipids And Lipoproteins Pathway Diagram
 PPAR Signaling Pathway antibodies  PPAR Signaling Pathway Diagram
 PPAR Signaling Pathway antibodies  PPAR Signaling Pathway Diagram
Diseases associated with FADS2 elisa kitOrgans/Tissues associated with FADS2 elisa kit
 Disease Name  Pubmed Publications
 Fatty Liver Antibodies  >48 publications with FADS2 and Fatty Liver
 Cardiovascular Diseases Antibodies  >24 publications with FADS2 and Cardiovascular Diseases
 Inflammation Antibodies  >17 publications with FADS2 and Inflammation
 Liver Diseases Antibodies  >9 publications with FADS2 and Liver Diseases
 Atherosclerosis Antibodies  >6 publications with FADS2 and Atherosclerosis
 Carcinoma, Hepatocellular Antibodies  >5 publications with FADS2 and Carcinoma, Hepatocellular
 Nervous System Diseases Antibodies  >4 publications with FADS2 and Nervous System Diseases
 Disease Models, Animal Antibodies  >4 publications with FADS2 and Disease Models, Animal
 Liver Neoplasms Antibodies  >3 publications with FADS2 and Liver Neoplasms
 Hypertension Antibodies  >3 publications with FADS2 and Hypertension
 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >53 publications with FADS2 and Liver
 Brain Antibodies  >25 publications with FADS2 and Brain
 Heart Antibodies  >19 publications with FADS2 and Heart
 Kidney Antibodies  >3 publications with FADS2 and Kidney
 Pancreas Antibodies  >2 publications with FADS2 and Pancreas
 Prostate Antibodies  >2 publications with FADS2 and Prostate
 Colon Antibodies  >2 publications with FADS2 and Colon
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