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FE elisa kit :: Rat Ferritin ELISA Kit

Scan QR to view Datasheet Catalog #    MBS722921
Typical Standard Curve/Testing Data
Unit / Price
48-Strip-Wells-(Competitive)  /  $455 +1 FREE 8GB USB
48-Strip-Wells-(Sandwich)  /  $455 +1 FREE 8GB USB
96-Strip-Wells-(Competitive)  /  $660 +1 FREE 8GB USB
96-Strip-Wells-(Sandwich)  /  $660 +1 FREE 8GB USB
5x96-Strip-Wells-(Competitive)  /  $2,925 +3 FREE 8GB USB
5x96-Strip-Wells-(Sandwich)  /  $2,925 +3 FREE 8GB USB
10x96-Strip-Wells-(Competitive)  /  $5,490 +6 FREE 8GB USB
10x96-Strip-Wells-(Sandwich)  /  $5,490 +6 FREE 8GB USB
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Ferritin, ELISA Kit

★Popular Item★
 Also Known As   

Rat Ferritin ELISA Kit

 Product Gene Name   

FE elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sandwich ELISA Manual Insert    Sandwich ELISA PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    gene 615517
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 Species Reactivity    Rat
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 Specificity    This assay has high sensitivity and excellent specificity for detection of FE. No significant cross-reactivity or interference between FE and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between FE and all the analogues, therefore, cross reaction may still exist in some cases.
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 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Assay Type    Competitive or Sandwich
 Detection Range    25-500ng/mL
 Sensitivity    1.0ng/ml
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of FE elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for FE purchase    MBS722921 is a ready-to-use microwell, strip plate Competitive or Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Ferritin, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 25-500ng/mL in biological research samples containing FE, with an estimated sensitivity of 1.0ng/ml. The ELISA analytical biochemical technique of the MBS722921 kit is based on FE antibody-FE antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect FE antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, FE. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for FE elisa kit

   Intended Uses: This FE ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat FE. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: FE ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for FE. Standards or samples are then added to the microtiter plate wells and FE if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of FE present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for FE are added to each well to "sandwich" the FE immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain FE and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FE concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for FE elisa kit    Neurobiology
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 Typical Standard Curve/Testing Data of FE elisa kit    FE elisa kit Typical Standard Curve/Testing Data image
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NCBI/Uniprot data below describe general gene information for FE. It may not necessarily be applicable to this product.
 NCBI GI #    306744
 NCBI GeneID    2495
 NCBI Accession #    AAA35832.1 [Other Products]
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 UniProt Secondary Accession #    Q3KRA8; Q3SWW1; B3KNR5 [Other Products]
 UniProt Related Accession #    P02794 [Other Products]
 Molecular Weight    21,226 Da
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 NCBI Official Full Name    ferritin
 NCBI Official Synonym Full Names    ferritin, heavy polypeptide 1
 NCBI Official Symbol    FTH1 [Similar Products]
 NCBI Official Synonym Symbols   
[Similar Products]
 NCBI Protein Information    ferritin heavy chain; apoferritin; ferritin H subunit; placenta immunoregulatory factor; proliferation-inducing protein 15; cell proliferation-inducing gene 15 protein
 UniProt Protein Name    Ferritin heavy chain
 UniProt Synonym Protein Names   
Cell proliferation-inducing gene 15 proteinCleaved into the following chain:Ferritin heavy chain, N-terminally processed
 Protein Family    Ferritin
 UniProt Gene Name    FTH1 [Similar Products]
 UniProt Synonym Gene Names    FTH; FTHL6; OK/SW-cl.84; PIG15; Ferritin H subunit [Similar Products]
 UniProt Entry Name    FRIH_HUMAN
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 NCBI Summary for FE    This gene encodes the heavy subunit of ferritin, the major intracellular iron storage protein in prokaryotes and eukaryotes. It is composed of 24 subunits of the heavy and light ferritin chains. Variation in ferritin subunit composition may affect the rates of iron uptake and release in different tissues. A major function of ferritin is the storage of iron in a soluble and nontoxic state. Defects in ferritin proteins are associated with several neurodegenerative diseases. This gene has multiple pseudogenes. Several alternatively spliced transcript variants have been observed, but their biological validity has not been determined. [provided by RefSeq, Jul 2008]
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 UniProt Comments for FE    Ferritin-H: Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Has ferroxidase activity. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney. Oligomer of 24 subunits. There are two types of subunits: L (light) chain and H (heavy) chain. The major chain can be light or heavy, depending on the species and tissue type. The functional molecule forms a roughly spherical shell with a diameter of 12 nm and contains a central cavity into which the insoluble mineral iron core is deposited. Belongs to the ferritin family.

Protein type: Cofactor and Vitamin Metabolism - porphyrin and chlorophyll; Oxidoreductase; EC

Chromosomal Location of Human Ortholog: 11q13

Cellular Component: ferritin complex; mitochondrion; cytosol; nucleus

Molecular Function: ferroxidase activity; protein binding; ferric iron binding; iron ion binding

Biological Process: receptor-mediated endocytosis; negative regulation of cell proliferation; negative regulation of fibroblast proliferation; cellular iron ion homeostasis; immune response; post-Golgi vesicle-mediated transport; transmembrane transport; iron ion transport; intracellular sequestering of iron ion

Disease: Hemochromatosis, Type 5
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 Research Articles on FE    1. Tumor necrosis factor-alpha attenuates starvation-induced apoptosis through upregulation of ferritin heavy chain in hepatocellular carcinoma cells.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with FE elisa kitPathways associated with FE elisa kit
 Reference Product  PubMed Publications
 FTL elisa kit  >12 publications with FE and FTL
 TFRC elisa kit  >3 publications with FE and TFRC
 FECH elisa kit  >2 publications with FE and FECH
 ACO1 elisa kit  >1 publications with FE and ACO1
 MAX elisa kit  >1 publications with FE and MAX
 MYC elisa kit  >1 publications with FE and MYC
 Products by Pathway  Pathway Diagram
 Binding And Uptake Of Ligands By Scavenger Receptors Pathway antibodies  Binding And Uptake Of Ligands By Scavenger Receptors Pathway Diagram
 Clathrin Derived Vesicle Budding Pathway antibodies  Clathrin Derived Vesicle Budding Pathway Diagram
 Golgi Associated Vesicle Biogenesis Pathway antibodies  Golgi Associated Vesicle Biogenesis Pathway Diagram
 Iron Metabolism In Placenta Pathway antibodies  Iron Metabolism In Placenta Pathway Diagram
 Iron Uptake And Transport Pathway antibodies  Iron Uptake And Transport Pathway Diagram
 Membrane Trafficking Pathway antibodies  Membrane Trafficking Pathway Diagram
 Mineral Absorption Pathway antibodies  Mineral Absorption Pathway Diagram
 Mineral Absorption Pathway antibodies  Mineral Absorption Pathway Diagram
 Scavenging By Class A Receptors Pathway antibodies  Scavenging By Class A Receptors Pathway Diagram
 Transmembrane Transport Of Small Molecules Pathway antibodies  Transmembrane Transport Of Small Molecules Pathway Diagram
Diseases associated with FE elisa kitOrgans/Tissues associated with FE elisa kit
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >5 publications with FE and Inflammation
 Liver Diseases Antibodies  >4 publications with FE and Liver Diseases
 Iron Overload Antibodies  >4 publications with FE and Iron Overload
 Disease Models, Animal Antibodies  >3 publications with FE and Disease Models, Animal
 Nervous System Diseases Antibodies  >3 publications with FE and Nervous System Diseases
 Brain Diseases Antibodies  >3 publications with FE and Brain Diseases
 Nerve Degeneration Antibodies  >2 publications with FE and Nerve Degeneration
 Neurodegenerative Diseases Antibodies  >2 publications with FE and Neurodegenerative Diseases
 Lung Neoplasms Antibodies  >1 publications with FE and Lung Neoplasms
 Edema Antibodies  >1 publications with FE and Edema
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >12 publications with FE and Blood
 Brain Antibodies  >9 publications with FE and Brain
 Liver Antibodies  >8 publications with FE and Liver
 Nerve Antibodies  >7 publications with FE and Nerve
 Placenta Antibodies  >4 publications with FE and Placenta
 Bone Antibodies  >4 publications with FE and Bone
 Spleen Antibodies  >3 publications with FE and Spleen
 Vascular Antibodies  >3 publications with FE and Vascular
 Bone Marrow Antibodies  >3 publications with FE and Bone Marrow
 Lung Antibodies  >3 publications with FE and Lung
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