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FGF elisa kit :: Rat Fibroblast Growth Factor (FGF) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS260671
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $275 +1 FREE 8GB USB
96-Strip-Wells  /  $435 +1 FREE 8GB USB
5x96-Strip-Wells  /  $1,755 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,150 +3 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Fibroblast Growth Factor (FGF), ELISA Kit

★Popular Item★
 Also Known As   

Rat Fibroblast Growth Factor (FGF) ELISA KIT

 Product Gene Name   

FGF elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    601513
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 Species Reactivity    Rat
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 Specificity    No cross-reaction with other factors.
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 Samples    Serum, plasma or cell culture supernatant
 Assay Type    Quantitative Sandwich
 Detection Range    1000 pg/ml-15.6 pg/ml
 Sensitivity    Up to 5pg/ml.
 Intra-assay Precision    <= 8%
 Inter-assay Precision    <= 12%
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of FGF elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for FGF purchase    MBS260671 is a ready-to-use microwell, strip plate Quantitative Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Fibroblast Growth Factor (FGF) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 1000 pg/ml-15.6 pg/ml in biological research samples containing FGF, with an estimated sensitivity of Up to 5pg/ml. The ELISA analytical biochemical technique of the MBS260671 kit is based on FGF antibody-FGF antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect FGF antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, FGF. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma or cell culture supernatant. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for FGF elisa kit

   Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Rat FGF monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
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 Typical Testing Data/Standard Curve (for reference only) of FGF elisa kit    FGF elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS260671. Click to request current manual
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NCBI/Uniprot data below describe general gene information for FGF. It may not necessarily be applicable to this product.
 NCBI GI #    5442453
 NCBI GeneID    2257
 NCBI Accession #    AAB18786.3 [Other Products]
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 UniProt Secondary Accession #    O35339; P70376; Q8TBG5; Q92912; Q93001; B2R6B7; B2R976 [Other Products]
 UniProt Related Accession #    P61328 [Other Products]
 Molecular Weight    20,424 Da
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 NCBI Official Full Name    fibroblast growth factor
 NCBI Official Synonym Full Names    fibroblast growth factor 12
 NCBI Official Symbol    FGF12 [Similar Products]
 NCBI Official Synonym Symbols   
FHF1; FGF12B
[Similar Products]
 NCBI Protein Information    fibroblast growth factor 12; FHF-1; FGF-12; myocyte-activating factor; fibroblast growth factor 12B; fibroblast growth factor FGF-12b; fibroblast growth factor homologous factor 1
 UniProt Protein Name    Fibroblast growth factor 12
 UniProt Synonym Protein Names   
Fibroblast growth factor homologous factor 1; FHF-1; Myocyte-activating factor
 Protein Family    Fibroblast growth factor
 UniProt Gene Name    FGF12 [Similar Products]
 UniProt Synonym Gene Names    FGF12B; FHF1; FGF-12; FHF-1 [Similar Products]
 UniProt Entry Name    FGF12_HUMAN
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 NCBI Summary for FGF    The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth, and invasion. This growth factor lacks the N-terminal signal sequence present in most of the FGF family members, but it contains clusters of basic residues that have been demonstrated to act as a nuclear localization signal. When transfected into mammalian cells, this protein accumulated in the nucleus, but was not secreted. The specific function of this gene has not yet been determined. Two alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]
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 UniProt Comments for FGF    FGF12: Probably involved in nervous system development and function. Belongs to the heparin-binding growth factors family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytokine; Cell development/differentiation

Chromosomal Location of Human Ortholog: 3q28

Cellular Component: extracellular space; nucleus

Molecular Function: heparin binding; sodium channel regulator activity; growth factor activity; fibroblast growth factor receptor binding

Biological Process: synaptic transmission; nervous system development; fibroblast growth factor receptor signaling pathway; cell-cell signaling; adult locomotory behavior; neuromuscular process; heart development; JNK cascade; signal transduction
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 Research Articles on FGF    1. Q7R-FGF12 is a disease-associated BrS mutation. Moreover, these data suggest for the first time that FHF effects on Na(+) and Ca(2+) channels are separable.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with FGF elisa kitPathways associated with FGF elisa kit
 Reference Product  PubMed Publications
 FGFR3 elisa kit  >3 publications with FGF and FGFR3
 FGFR2 elisa kit  >3 publications with FGF and FGFR2
 FGFR1 elisa kit  >2 publications with FGF and FGFR1
 FGFR4 elisa kit  >2 publications with FGF and FGFR4
 IGF1R elisa kit  >1 publications with FGF and IGF1R
 Products by Pathway  Pathway Diagram
 MAPK Signaling Pathway antibodies  MAPK Signaling Pathway Diagram
 MAPK Signaling Pathway antibodies  MAPK Signaling Pathway Diagram
 Melanoma Pathway antibodies  Melanoma Pathway Diagram
 Melanoma Pathway antibodies  Melanoma Pathway Diagram
 PI3K-Akt Signaling Pathway antibodies  PI3K-Akt Signaling Pathway Diagram
 PI3K-Akt Signaling Pathway antibodies  PI3K-Akt Signaling Pathway Diagram
 Pathways In Cancer antibodies  Pathways In Cancer Diagram
 Proteoglycans In Cancer Pathway antibodies  Proteoglycans In Cancer Pathway Diagram
 Proteoglycans In Cancer Pathway antibodies  Proteoglycans In Cancer Pathway Diagram
 Regulation Of Actin Cytoskeleton Pathway antibodies  Regulation Of Actin Cytoskeleton Pathway Diagram
Diseases associated with FGF elisa kitOrgans/Tissues associated with FGF elisa kit
 Disease Name  Pubmed Publications
 Congenital Abnormalities Antibodies  >4 publications with FGF and Congenital Abnormalities
 Cleft Lip Antibodies  >3 publications with FGF and Cleft Lip
 Cleft Palate Antibodies  >3 publications with FGF and Cleft Palate
 Breast Neoplasms Antibodies  >2 publications with FGF and Breast Neoplasms
 Lung Neoplasms Antibodies  >1 publications with FGF and Lung Neoplasms
 Heart Defects, Congenital Antibodies  >1 publications with FGF and Heart Defects, Congenital
 Drug Toxicity Antibodies  >1 publications with FGF and Drug Toxicity
 Fibrosis Antibodies  >1 publications with FGF and Fibrosis
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >8 publications with FGF and Brain
 Embryonic Tissue Antibodies  >5 publications with FGF and Embryonic Tissue
 Heart Antibodies  >3 publications with FGF and Heart
 Ganglia Antibodies  >2 publications with FGF and Ganglia
 Pituitary Gland Antibodies  >1 publications with FGF and Pituitary Gland
 Lung Antibodies  >1 publications with FGF and Lung
 Kidney Antibodies  >1 publications with FGF and Kidney
 Mouth Antibodies  >1 publications with FGF and Mouth
 Eye Antibodies  >1 publications with FGF and Eye
 Connective Tissue Antibodies  >1 publications with FGF and Connective Tissue
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