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FN elisa kit :: Rat Fibronectin / FN ELISA Kit

Scan QR to view Datasheet Catalog #    MBS355294
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
96 Tests  /  $440 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Fibronectin / FN, ELISA Kit

 Also Known As   

Fibronectin / FN Rat ELISA Kit

 Product Synonym Names    Rat Fibronectin / FN
 Product Gene Name   

FN elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Manual Insert    Download PDF Manual View PDF Manual
 Chromosome Location    Chromosome: 2; NC_000002.11 (216225177..216300890, complement). Location: 2q34
 OMIM    135600
 3D Structure    ModBase 3D Structure for P02751
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 Species Reactivity    Rat
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 Detection Range    156 pg/ml-10,000 pg/ml
 Sensitivity    < 15 pg/ml
 Assay Type    Sandwich
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 Preparation and Storage    Store at 2-8 degree C for 4 months, or at -20 degree C for 8 months.
 Product Note    Our ELISA assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay.
 Other Notes    Small volumes of FN elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for FN purchase    MBS355294 is a ready-to-use microwell, strip-or-full plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Fibronectin / FN, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 156 pg/ml-10,000 pg/ml in biological research samples containing FN, with an estimated sensitivity of < 15 pg/ml. The ELISA analytical biochemical technique of the MBS355294 kit is based on FN antibody-FN antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect FN antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, FN. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for FN elisa kit

   Background/Introduction: Fibronectin is a high-molecular weight (~440kDa) glycoprotein of the extracellular matrix, belongs to a family of high molecular weight glycoproteins that are present on cell surfaces, in extracellular fluids, connective tissues, and basement membranes. Two major forms of fibronectin exist: a plasma soluble form and a cellular form. It plays a major role in cell adhesion, growth, migration, and differentiation, and it is important for processes such as wound healing and embryonic development. Fibronectin has been implicated in carcinoma development, and also acts as a potential biomarker for radioresistance.

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Fibronectin polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Fibronectin polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Fibronectin amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Fibronectin can be calculated.
 Applications Tested/Suitable for FN elisa kit   

Quantitative sELISA (EIA)

 Application Notes for FN elisa kit    For quantitative detection of Fibronectin in rat serum, plasma, body fluids, tissue lysates or cell culture supernatants.
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 Typical Testing Data/Standard Curve (for reference only) of FN elisa kit    FN elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS355294
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NCBI/Uniprot data below describe general gene information for FN. It may not necessarily be applicable to this product.
 NCBI GI #    15866738
 NCBI GeneID    2335
 NCBI Accession #    CAC86915.1 [Other Products]
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 UniProt Primary Accession #    P02751 [Other Products]
 UniProt Secondary Accession #    O95609; O95610; Q14312; Q14325; Q14326; Q17RV7; Q564H7; Q585T2; B7ZLF0; E9PE77; E9PG29 [Other Products]
 UniProt Related Accession #    P02751 [Other Products]
 Molecular Weight    262,625 Da
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 NCBI Official Full Name    fibronectin
 NCBI Official Synonym Full Names    fibronectin 1
 NCBI Official Symbol    FN1 [Similar Products]
 NCBI Official Synonym Symbols   
FN; CIG; FNZ; MSF; ED-B; FINC; GFND; LETS; GFND2
[Similar Products]
 NCBI Protein Information    fibronectin; cold-insoluble globulin; migration-stimulating factor
 UniProt Protein Name    Fibronectin
 UniProt Synonym Protein Names   
Cold-insoluble globulin
 UniProt Gene Name    FN1 [Similar Products]
 UniProt Synonym Gene Names    FN; FN; CIG [Similar Products]
 UniProt Entry Name    FINC_HUMAN
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 NCBI Summary for FN    This gene encodes fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. The gene has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants. However, the full-length nature of some variants has not been determined. [provided by RefSeq, Jul 2008]
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 UniProt Comments for FN    Function: Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts. Ref.38 Ref.40 Ref.44 Ref.50Anastellin binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling. Ref.38 Ref.40 Ref.44 Ref.50

Subunit structure: Mostly heterodimers or multimers of alternatively spliced variants, connected by 2 disulfide bonds near the carboxyl ends; to a lesser extent homodimers. Interacts with FBLN1, AMBP, TNR, LGALS3BP and COL13A1. Interacts with FBLN7

By similarity. Interacts with COMP. Interacts with S.aureus fnbA. Interacts with TNR; the interaction inhibits cell adhesion and neurite outgrowth

By similarity. Interacts with FST3. Ref.36 Ref.38 Ref.39 Ref.41 Ref.42 Ref.47

Subcellular location: Secreted › extracellular space › extracellular matrix.

Tissue specificity: Plasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. Ugl-Y1, Ugl-Y2 and Ugl-Y3 are found in urine. Ref.16 Ref.35

Developmental stage: Ugl-Y1, Ugl-Y2 and Ugl-Y3 are present in the urine from 0 to 17 years of age. Ref.16 Ref.35

Post-translational modification: Sulfated.It is not known whether both or only one of Thr-2064 and Thr-2065 are/is glycosylated. Ref.16 Ref.26 Ref.35 Ref.58Forms covalent cross-links mediated by a transglutaminase, such as F13A or TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers (e.g. fibrinogen-fibronectin, collagen-fibronectin heteropolymers).Phosphorylation sites are present in the extracellular medium.Proteolytic processing produces the C-terminal NC1 peptide, anastellin.

Involvement in disease: Glomerulopathy with fibronectin deposits 2 (GFND2) [MIM:601894]: Genetically heterogeneous autosomal dominant disorder characterized clinically by proteinuria, microscopic hematuria, and hypertension that leads to end-stage renal failure in the second to fifth decade of life.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.72

Sequence similarities: Contains 12 fibronectin type-I domains.Contains 2 fibronectin type-II domains.Contains 16 fibronectin type-III domains.

Sequence caution: The sequence AAX76513.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence BAD93077.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.The sequence CAD91166.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.The sequence CAD97964.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.The sequence CAD97965.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.The sequence CAH18136.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.
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 Research Articles on FN    1. Data indicate that metastatic breast cancer (BC) cells that failed to activate STAT3 downstream of EGFR did display robust STAT3 activity upon adhesion to fibronectin (FN).
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products related to Fibronectin / FN Rat ELISA Kit
 Catalog #  Product Name  Catalog #  Product Name
 MBS495419  Fibronectin / FN ELISA Kit  MBS495303  Fibronectin / FN (Rat) ELISA Kit
 MBS355294  Fibronectin / FN Rat ELISA Kit  MBS355349  Fibronectin / FN Mouse ELISA Kit
 MBS355273  Fibronectin / FN Human ELISA Kit
Pathways associated with FN elisa kitDiseases associated with FN elisa kit
 Products by Pathway  Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Angiopoietin Receptor Tie2-mediated Signaling Pathway antibodies  Angiopoietin Receptor Tie2-mediated Signaling Pathway Diagram
 Bacterial Invasion Of Epithelial Cells Pathway antibodies  Bacterial Invasion Of Epithelial Cells Pathway Diagram
 Bacterial Invasion Of Epithelial Cells Pathway antibodies  Bacterial Invasion Of Epithelial Cells Pathway Diagram
 Cell Surface Interactions At The Vascular Wall Pathway antibodies  Cell Surface Interactions At The Vascular Wall Pathway Diagram
 ECM-receptor Interaction Pathway antibodies  ECM-receptor Interaction Pathway Diagram
 ECM-receptor Interaction Pathway antibodies  ECM-receptor Interaction Pathway Diagram
 Extracellular Matrix Organization Pathway antibodies  Extracellular Matrix Organization Pathway Diagram
 Fibronectin Matrix Formation Pathway antibodies  Fibronectin Matrix Formation Pathway Diagram
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >85 publications with FN and Neoplasms
 Carcinoma Antibodies  >52 publications with FN and Carcinoma
 Fibrosis Antibodies  >18 publications with FN and Fibrosis
 Inflammation Antibodies  >14 publications with FN and Inflammation
 Neoplasms, Experimental Antibodies  >14 publications with FN and Neoplasms, Experimental
 Lung Neoplasms Antibodies  >13 publications with FN and Lung Neoplasms
 Cardiovascular Diseases Antibodies  >11 publications with FN and Cardiovascular Diseases
 Kidney Diseases Antibodies  >9 publications with FN and Kidney Diseases
 Diabetes Mellitus Antibodies  >8 publications with FN and Diabetes Mellitus
 Liver Diseases Antibodies  >6 publications with FN and Liver Diseases
Organs/Tissues associated with FN elisa kit
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >37 publications with FN and Blood
 Lung Antibodies  >21 publications with FN and Lung
 Muscle Antibodies  >20 publications with FN and Muscle
 Bone Antibodies  >20 publications with FN and Bone
 Kidney Antibodies  >18 publications with FN and Kidney
 Embryonic Tissue Antibodies  >17 publications with FN and Embryonic Tissue
 Brain Antibodies  >15 publications with FN and Brain
 Vascular Antibodies  >15 publications with FN and Vascular
 Liver Antibodies  >14 publications with FN and Liver
 Connective Tissue Antibodies  >14 publications with FN and Connective Tissue
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