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FN1 elisa kit :: Rat Fibronectin ELISA Kit

Scan QR to view Datasheet Catalog #    MBS824794
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
96 Tests  /  $470 +1 FREE 8GB USB
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 Product Name   

Fibronectin (FN1), ELISA Kit

★Popular Item★
 Also Known As   

Rat Fibronectin ELISA Kit

 Product Synonym Names    FN; Fibronectin; FN; Cold-insoluble globulin; CIG
 Product Gene Name   

FN1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 3D Structure    ModBase 3D Structure for P04937
 Species Reactivity    Rat
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 Specificity    The Rat Fibronectin ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Rat Fibronectin proteins within the range of 156 pg/ml - 10000 pg/ml.
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 Samples    Cell Culture Supernatants, Serum, Plasma
 Sensitivity    <15 pg/ml
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 Preparation and Storage    Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of FN1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for FN1 purchase    MBS824794 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Fibronectin (FN1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing FN1, with an estimated sensitivity of <15 pg/ml. The ELISA analytical biochemical technique of the MBS824794 kit is based on FN1 antibody-FN1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect FN1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, FN1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Cell Culture Supernatants, Serum, Plasma. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for FN1 elisa kit

   Principle of the Assay: The Rat Fibronectin ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat Fibronectin in Cell Culture Supernatants, Serum, Plasma. This assay employs an antibody specific for Rat Fibronectin coated on a 96-well plate. Standards and samples are pipetted into the wells and Fibronectin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Rat Fibronectin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Fibronectin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Background: Fibronectin (FN) also known as LETS, is identified on the surfFN of fibroblasts by labeling with radioactive compounds or specific antibodies. Fibronectin is a 430,000-dalton dimeric glycoprotein that exists in 2 forms, termed cellular and plasma fibronectin. Cellular and plasma fibronectins are heterodimers consisting of similar but not identical polypeptides. These two forms of FN differ in biologic activity. Fibronectins bind cell surfFNs and various compounds including collagen, fibrin, heparin, DNA, and actin. Because fibronectin stimulates endocytosis in several systems and promotes the clearance of particulate material from the circulation, it could function in the clearance of C1q-coated material such as immune complexes or cellular debris. Fibronectins are involved in cell adhesion, cell motility, opsonization, would healing, and maintenance of cell shape. LETS, encoded on chromosome 8, is responsible for the LETS protein expression in humans. Because LETS has been implicated in tumorigenicity and cellular transformation, it is of interest that rearrangement or modifications in the number of chromosome 8 have been associated with certain forms of cancer.
 Applications Tested/Suitable for FN1 elisa kit   

Sandwich ELISA (SE)

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 Typical Testing Data/Standard Curve (for reference only) of FN1 elisa kit    FN1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS824794. Click to request current manual
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NCBI/Uniprot data below describe general gene information for FN1. It may not necessarily be applicable to this product.
 NCBI GI #    120178
 NCBI GeneID    25661
 NCBI Accession #    P04937.2 [Other Products]
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 UniProt Primary Accession #    P04937 [Other Products]
 UniProt Secondary Accession #    Q6LDX9 [Other Products]
 UniProt Related Accession #    P04937 [Other Products]
 Molecular Weight    259,350 Da
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 NCBI Official Full Name    Fibronectin
 NCBI Official Synonym Full Names    fibronectin 1
 NCBI Official Symbol    Fn1 [Similar Products]
 NCBI Official Synonym Symbols   
fn-1; FIBNEC
[Similar Products]
 NCBI Protein Information    fibronectin; FN
 UniProt Protein Name    Fibronectin
 Protein Family    Fibronectin
 UniProt Gene Name    Fn1 [Similar Products]
 UniProt Synonym Gene Names    FN [Similar Products]
 UniProt Entry Name    FINC_RAT
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 NCBI Summary for FN1    extracellular matrix component; may play a role in fibrosis and tumor metastasis [RGD, Feb 2006]
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 UniProt Comments for FN1    FN1: Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Defects in FN1 are the cause of glomerulopathy with fibronectin deposits type 2 (GFND2); also known as familial glomerular nephritis with fibronectin deposits or fibronectin glomerulopathy. GFND is a genetically heterogeneous autosomal dominant disorder characterized clinically by proteinuria, microscopic hematuria, and hypertension that leads to end-stage renal failure in the second to fifth decade of life. 15 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Secreted; Cell adhesion; Secreted, signal peptide

Cellular Component: extracellular matrix; extracellular space; proteinaceous extracellular matrix; apical plasma membrane; fibrinogen complex; ER-Golgi intermediate compartment; extracellular region; basement membrane; basal lamina

Molecular Function: integrin binding; heparin binding; protein binding; protease activator activity; protease binding; extracellular matrix structural constituent; mercury ion binding

Biological Process: integrin activation; extracellular matrix organization and biogenesis; ossification; wound healing; positive regulation of chemotaxis; positive regulation of axon extension; cell-matrix adhesion; response to glucocorticoid stimulus; glial cell migration; cell activation; regulation of cell shape; response to ozone; cell-substrate junction assembly; acute-phase response; calcium-independent cell-matrix adhesion; response to wounding; peptide cross-linking; angiogenesis; cell adhesion; negative regulation of apoptosis; positive regulation of cell migration
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 Research Articles on FN1    1. Uncoupled eNOS is responsible for Ang II-induced mesangial cell fibronectin accumulation and identifies Nox4 and mitochondrial reactive oxygen species as mediators of eNOS dysfunction.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with FN1 elisa kitOrgans/Tissues associated with FN1 elisa kit
 Products by Pathway  Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Bacterial Invasion Of Epithelial Cells Pathway antibodies  Bacterial Invasion Of Epithelial Cells Pathway Diagram
 Bacterial Invasion Of Epithelial Cells Pathway antibodies  Bacterial Invasion Of Epithelial Cells Pathway Diagram
 Cell Surface Interactions At The Vascular Wall Pathway antibodies  Cell Surface Interactions At The Vascular Wall Pathway Diagram
 ECM-receptor Interaction Pathway antibodies  ECM-receptor Interaction Pathway Diagram
 ECM-receptor Interaction Pathway antibodies  ECM-receptor Interaction Pathway Diagram
 Extracellular Matrix Organization Pathway antibodies  Extracellular Matrix Organization Pathway Diagram
 Fibronectin Matrix Formation Pathway antibodies  Fibronectin Matrix Formation Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >27 publications with FN1 and Lung
 Muscle Antibodies  >24 publications with FN1 and Muscle
 Kidney Antibodies  >24 publications with FN1 and Kidney
 Liver Antibodies  >22 publications with FN1 and Liver
 Embryonic Tissue Antibodies  >19 publications with FN1 and Embryonic Tissue
 Brain Antibodies  >17 publications with FN1 and Brain
 Connective Tissue Antibodies  >15 publications with FN1 and Connective Tissue
 Colon Antibodies  >9 publications with FN1 and Colon
 Heart Antibodies  >8 publications with FN1 and Heart
 Prostate Antibodies  >7 publications with FN1 and Prostate
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