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HGF elisa kit :: Rat HGF ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2510475 (SPECIAL PROMOTIONAL PRICING for a limited time)
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $310 +1 FREE 8GB USB
96-Strip-Wells  /  $355 +1 FREE 8GB USB
5x96-Strip-Wells  /  $1,435 +1 FREE 8GB USB
10x96-Strip-Wells  /  $2,805 +3 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

HGF, ELISA Kit

★Popular Item★
 Also Known As   

Rat HGF (Hepatocyte Growth Factor) ELISA Kit

 Product Gene Name   

HGF elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    142409
 3D Structure    ModBase 3D Structure for P14210
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 Species Reactivity    Rat
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Rat HGF. No significant cross-reactivity or interference between Rat HGF and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Rat HGF and all the analogues, therefore, cross reaction may still exist.
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 Samples    Serum, Plasma, Biological Fluids
 Assay Type    Sandwich
 Detection Range    62.5-4000pg/mL
 Sensitivity    Min: 37.5pg/mL; Max: 4000pg/mL
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 Preparation and Storage    Store at 4 degree C.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of HGF elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for HGF purchase    MBS2510475 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the HGF, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 62.5-4000pg/mL in biological research samples containing HGF, with an estimated sensitivity of Min: 37.5pg/mL; Max: 4000pg/mL. The ELISA analytical biochemical technique of the MBS2510475 kit is based on HGF antibody-HGF antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect HGF antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, HGF. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma, Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for HGF elisa kit

   Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of HGF in Rat serum, plasma and other biological fluids
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat HGF. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Rat HGF and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat HGF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Rat HGF. You can calculate the concentration of Rat HGF in the samples by comparing the OD of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of HGF elisa kit    HGF elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2510475. Click to request current manual
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NCBI/Uniprot data below describe general gene information for HGF. It may not necessarily be applicable to this product.
 NCBI GI #    33859835
 NCBI GeneID    3082
 NCBI Accession #    NP_000592.3 [Other Products]
 NCBI GenBank Nucleotide #    NM_000601.4 [Other Products]
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 UniProt Primary Accession #    P14210 [Other Products]
 UniProt Secondary Accession #    Q02935; Q13494; Q14519; Q3KRB2; Q8TCE2; Q9BYL9; Q9BYM0; Q9UDU6; A1L3U6 [Other Products]
 UniProt Related Accession #    P14210 [Other Products]
 Molecular Weight    24,116 Da
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 NCBI Official Full Name    hepatocyte growth factor isoform 1 preproprotein
 NCBI Official Synonym Full Names    hepatocyte growth factor (hepapoietin A; scatter factor)
 NCBI Official Symbol    HGF [Similar Products]
 NCBI Official Synonym Symbols   
SF; HGFB; HPTA; F-TCF; DFNB39
[Similar Products]
 NCBI Protein Information    hepatocyte growth factor; fibroblast-derived tumor cytotoxic factor; hepatopoeitin-A; hepatopoietin-A; lung fibroblast-derived mitogen
 UniProt Protein Name    Hepatocyte growth factor
 UniProt Synonym Protein Names   
Hepatopoietin-A; Scatter factor; SF
 Protein Family    Hepatocyte growth factor
 UniProt Gene Name    HGF [Similar Products]
 UniProt Synonym Gene Names    HPTA; SF [Similar Products]
 UniProt Entry Name    HGF_HUMAN
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 NCBI Summary for HGF    Hepatocyte growth factor regulates cell growth, cell motility, and morphogenesis by activating a tyrosine kinase signaling cascade after binding to the proto-oncogenic c-Met receptor. Hepatocyte growth factor is secreted by mesenchymal cells and acts as a multi-functional cytokine on cells of mainly epithelial origin. Its ability to stimulate mitogenesis, cell motility, and matrix invasion gives it a central role in angiogenesis, tumorogenesis, and tissue regeneration. It is secreted as a single inactive polypeptide and is cleaved by serine proteases into a 69-kDa alpha-chain and 34-kDa beta-chain. A disulfide bond between the alpha and beta chains produces the active, heterodimeric molecule. The protein belongs to the plasminogen subfamily of S1 peptidases but has no detectable protease activity. Alternative splicing of this gene produces multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
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 UniProt Comments for HGF    HGF: HGF is a potent mitogen for mature parenchymal hepatocyte cells, seems to be an hepatotrophic factor, and acts as growth factor for a broad spectrum of tissues and cell types. It has no detectable protease activity. Activating ligand for the receptor tyrosine kinase MET by binding and promoting its dimerization. Defects in HGF are the cause of deafness autosomal recessive type 39 (DFNB39). A form of profound prelingual sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information. Belongs to the peptidase S1 family. Plasminogen subfamily. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell development/differentiation; Motility/polarity/chemotaxis; Hormone

Chromosomal Location of Human Ortholog: 7q21.1

Cellular Component: extracellular space; membrane; extracellular region

Molecular Function: identical protein binding; protein binding; growth factor activity; protein heterodimerization activity; serine-type endopeptidase activity; chemoattractant activity

Biological Process: positive regulation of myelination; platelet activation; mitosis; organ regeneration; activation of MAPK activity; myoblast proliferation; hepatocyte growth factor receptor signaling pathway; negative regulation of caspase activity; proteolysis; liver development; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of osteoblast differentiation; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of angiogenesis; platelet degranulation; positive chemotaxis; epithelial to mesenchymal transition; positive regulation of transcription from RNA polymerase II promoter; blood coagulation; hyaluronan metabolic process; positive regulation of cell migration

Disease: Deafness, Autosomal Recessive 39
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 Research Articles on HGF    1. induction of HGF synthesis via RNA transferred by microvesicles activating AKT and ERK1/2 signaling is a crucial contributor to the pro-tumor effect
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with HGF elisa kitDiseases associated with HGF elisa kit
 Products by Pathway  Pathway Diagram
 Arf6 Signaling Events Pathway antibodies  Arf6 Signaling Events Pathway Diagram
 Cytokine Signaling In Immune System Pathway antibodies  Cytokine Signaling In Immune System Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Direct P53 Effectors Pathway antibodies  Direct P53 Effectors Pathway Diagram
 FGF Signaling Pathway antibodies  FGF Signaling Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
 Hemostasis Pathway antibodies  Hemostasis Pathway Diagram
 Disease Name  Pubmed Publications
 Liver Diseases Antibodies  >759 publications with HGF and Liver Diseases
 Disease Models, Animal Antibodies  >623 publications with HGF and Disease Models, Animal
 Vascular Diseases Antibodies  >602 publications with HGF and Vascular Diseases
 Necrosis Antibodies  >457 publications with HGF and Necrosis
 Fibrosis Antibodies  >442 publications with HGF and Fibrosis
 Liver Neoplasms Antibodies  >433 publications with HGF and Liver Neoplasms
 Neoplasm Invasiveness Antibodies  >427 publications with HGF and Neoplasm Invasiveness
 Inflammation Antibodies  >420 publications with HGF and Inflammation
 Neoplasms, Experimental Antibodies  >302 publications with HGF and Neoplasms, Experimental
 Kidney Diseases Antibodies  >299 publications with HGF and Kidney Diseases
Organs/Tissues associated with HGF elisa kit
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >1815 publications with HGF and Blood
 Liver Antibodies  >1681 publications with HGF and Liver
 Vascular Antibodies  >1235 publications with HGF and Vascular
 Bone Antibodies  >755 publications with HGF and Bone
 Kidney Antibodies  >746 publications with HGF and Kidney
 Lung Antibodies  >677 publications with HGF and Lung
 Muscle Antibodies  >608 publications with HGF and Muscle
 Bone Marrow Antibodies  >528 publications with HGF and Bone Marrow
 Embryonic Tissue Antibodies  >376 publications with HGF and Embryonic Tissue
 Brain Antibodies  >349 publications with HGF and Brain
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